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Bazooka/PAR3 is dispensable for polarity in Drosophila follicular epithelial cells.

Shahab J, Tiwari MD, Honemann-Capito M, Krahn MP, Wodarz A - Biol Open (2015)

Bottom Line: While all these baz alleles display identical phenotypes during embryonic epithelial development, we observe strong discrepancies in the severity and penetrance of polarity defects in the follicular epithelium: polarity is mostly normal in baz(EH747) and baz(XR11) while baz(4) and baz(815) (-8) show loss of polarity, severe multilayering and loss of epithelial integrity throughout the clones.Further analysis reveals that the chromosomes carrying the baz(4) and baz(815-8) alleles may contain additional mutations that enhance the true baz loss-of-function phenotype in the follicular epithelium.This study clearly shows that Baz is dispensable for the regulation of polarity in the follicular epithelium, and that the requirement for key regulators of cell polarity is highly dependent on developmental context and cell type.

View Article: PubMed Central - PubMed

Affiliation: Stammzellbiologie, Institut für Anatomie und Zellbiologie, Georg-August Universität Göttingen, Justus-von-Liebig-Weg 11, 37077 Göttingen, Germany.

No MeSH data available.


Related in: MedlinePlus

Baz and Cno do not regulate the localization of one another in the FE.Ovarian follicles in which, bazEH747 (A–A″″, Stage 6) and baz815-8 (B–B″″, Stage 8) clones induced by hs-Flp mediated recombination are marked by loss of His-GFP. White boxes in A,B indicate regions shown in A′–A″″ and B′–B″″, respectively. Follicles were stained with antibodies against Par6 and Sdt as indicated. No effect is seen on Par6 and Sdt localization at the apical membrane in bazEH747 (A″,A′″) mutant cells. On the other hand, baz815-8 (B″,B′″) mutant cells show coincident loss of Par6 and Sdt from the apical membrane. However, apical Par6 and Sdt staining is still visible in many baz815-8 mutant cells (B″–B″″) as indicated by white arrowheads. (C–C″″) FE clones for bazEH747 (stage 8) marked by absence of GFP and generated using the directed mosaic system by expressing UAS-Flp under the control of e22c-Gal4, immunostained with Cno, Arm and GFP antibodies as indicated. (D–D″″) cnoR2 clones (stage 7) immunostained with Baz, Lgl and GFP antibodies as indicated, marked by absence of GFP, were generated using the directed mosaic system by expressing UAS-Flp under the control of e22c-Gal4. White boxes in C and D indicate regions shown in C′–C″″ and D′–D″″, respectively. No effect is seen on Cno or Arm apical localization in bazEH747 mutant FE clones (C–C″″) or Baz localization in cnoR2 mutant FE clones (D–D″″). Scale bars = 20 µm (A–D); 5 µm (all other scale bars).
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f08: Baz and Cno do not regulate the localization of one another in the FE.Ovarian follicles in which, bazEH747 (A–A″″, Stage 6) and baz815-8 (B–B″″, Stage 8) clones induced by hs-Flp mediated recombination are marked by loss of His-GFP. White boxes in A,B indicate regions shown in A′–A″″ and B′–B″″, respectively. Follicles were stained with antibodies against Par6 and Sdt as indicated. No effect is seen on Par6 and Sdt localization at the apical membrane in bazEH747 (A″,A′″) mutant cells. On the other hand, baz815-8 (B″,B′″) mutant cells show coincident loss of Par6 and Sdt from the apical membrane. However, apical Par6 and Sdt staining is still visible in many baz815-8 mutant cells (B″–B″″) as indicated by white arrowheads. (C–C″″) FE clones for bazEH747 (stage 8) marked by absence of GFP and generated using the directed mosaic system by expressing UAS-Flp under the control of e22c-Gal4, immunostained with Cno, Arm and GFP antibodies as indicated. (D–D″″) cnoR2 clones (stage 7) immunostained with Baz, Lgl and GFP antibodies as indicated, marked by absence of GFP, were generated using the directed mosaic system by expressing UAS-Flp under the control of e22c-Gal4. White boxes in C and D indicate regions shown in C′–C″″ and D′–D″″, respectively. No effect is seen on Cno or Arm apical localization in bazEH747 mutant FE clones (C–C″″) or Baz localization in cnoR2 mutant FE clones (D–D″″). Scale bars = 20 µm (A–D); 5 µm (all other scale bars).

Mentions: We also analyzed the distribution of Par6 and Sdt, members of the Par complex and Crumbs complex, respectively, in bazEH747 and baz815-8 FE clones (Fig. 8A,B). Again, we observed no effect on the localization of these proteins in bazEH747 FE clones. However, immunostaining for Par6 and Sdt in baz815-8 FE clones (Fig. 8A,B) and baz4 FE clones (data not shown) showed results similar to those obtained for aPKC and Crumbs.


Bazooka/PAR3 is dispensable for polarity in Drosophila follicular epithelial cells.

Shahab J, Tiwari MD, Honemann-Capito M, Krahn MP, Wodarz A - Biol Open (2015)

Baz and Cno do not regulate the localization of one another in the FE.Ovarian follicles in which, bazEH747 (A–A″″, Stage 6) and baz815-8 (B–B″″, Stage 8) clones induced by hs-Flp mediated recombination are marked by loss of His-GFP. White boxes in A,B indicate regions shown in A′–A″″ and B′–B″″, respectively. Follicles were stained with antibodies against Par6 and Sdt as indicated. No effect is seen on Par6 and Sdt localization at the apical membrane in bazEH747 (A″,A′″) mutant cells. On the other hand, baz815-8 (B″,B′″) mutant cells show coincident loss of Par6 and Sdt from the apical membrane. However, apical Par6 and Sdt staining is still visible in many baz815-8 mutant cells (B″–B″″) as indicated by white arrowheads. (C–C″″) FE clones for bazEH747 (stage 8) marked by absence of GFP and generated using the directed mosaic system by expressing UAS-Flp under the control of e22c-Gal4, immunostained with Cno, Arm and GFP antibodies as indicated. (D–D″″) cnoR2 clones (stage 7) immunostained with Baz, Lgl and GFP antibodies as indicated, marked by absence of GFP, were generated using the directed mosaic system by expressing UAS-Flp under the control of e22c-Gal4. White boxes in C and D indicate regions shown in C′–C″″ and D′–D″″, respectively. No effect is seen on Cno or Arm apical localization in bazEH747 mutant FE clones (C–C″″) or Baz localization in cnoR2 mutant FE clones (D–D″″). Scale bars = 20 µm (A–D); 5 µm (all other scale bars).
© Copyright Policy - open-access
Related In: Results  -  Collection

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f08: Baz and Cno do not regulate the localization of one another in the FE.Ovarian follicles in which, bazEH747 (A–A″″, Stage 6) and baz815-8 (B–B″″, Stage 8) clones induced by hs-Flp mediated recombination are marked by loss of His-GFP. White boxes in A,B indicate regions shown in A′–A″″ and B′–B″″, respectively. Follicles were stained with antibodies against Par6 and Sdt as indicated. No effect is seen on Par6 and Sdt localization at the apical membrane in bazEH747 (A″,A′″) mutant cells. On the other hand, baz815-8 (B″,B′″) mutant cells show coincident loss of Par6 and Sdt from the apical membrane. However, apical Par6 and Sdt staining is still visible in many baz815-8 mutant cells (B″–B″″) as indicated by white arrowheads. (C–C″″) FE clones for bazEH747 (stage 8) marked by absence of GFP and generated using the directed mosaic system by expressing UAS-Flp under the control of e22c-Gal4, immunostained with Cno, Arm and GFP antibodies as indicated. (D–D″″) cnoR2 clones (stage 7) immunostained with Baz, Lgl and GFP antibodies as indicated, marked by absence of GFP, were generated using the directed mosaic system by expressing UAS-Flp under the control of e22c-Gal4. White boxes in C and D indicate regions shown in C′–C″″ and D′–D″″, respectively. No effect is seen on Cno or Arm apical localization in bazEH747 mutant FE clones (C–C″″) or Baz localization in cnoR2 mutant FE clones (D–D″″). Scale bars = 20 µm (A–D); 5 µm (all other scale bars).
Mentions: We also analyzed the distribution of Par6 and Sdt, members of the Par complex and Crumbs complex, respectively, in bazEH747 and baz815-8 FE clones (Fig. 8A,B). Again, we observed no effect on the localization of these proteins in bazEH747 FE clones. However, immunostaining for Par6 and Sdt in baz815-8 FE clones (Fig. 8A,B) and baz4 FE clones (data not shown) showed results similar to those obtained for aPKC and Crumbs.

Bottom Line: While all these baz alleles display identical phenotypes during embryonic epithelial development, we observe strong discrepancies in the severity and penetrance of polarity defects in the follicular epithelium: polarity is mostly normal in baz(EH747) and baz(XR11) while baz(4) and baz(815) (-8) show loss of polarity, severe multilayering and loss of epithelial integrity throughout the clones.Further analysis reveals that the chromosomes carrying the baz(4) and baz(815-8) alleles may contain additional mutations that enhance the true baz loss-of-function phenotype in the follicular epithelium.This study clearly shows that Baz is dispensable for the regulation of polarity in the follicular epithelium, and that the requirement for key regulators of cell polarity is highly dependent on developmental context and cell type.

View Article: PubMed Central - PubMed

Affiliation: Stammzellbiologie, Institut für Anatomie und Zellbiologie, Georg-August Universität Göttingen, Justus-von-Liebig-Weg 11, 37077 Göttingen, Germany.

No MeSH data available.


Related in: MedlinePlus