Limits...
Bazooka/PAR3 is dispensable for polarity in Drosophila follicular epithelial cells.

Shahab J, Tiwari MD, Honemann-Capito M, Krahn MP, Wodarz A - Biol Open (2015)

Bottom Line: While all these baz alleles display identical phenotypes during embryonic epithelial development, we observe strong discrepancies in the severity and penetrance of polarity defects in the follicular epithelium: polarity is mostly normal in baz(EH747) and baz(XR11) while baz(4) and baz(815) (-8) show loss of polarity, severe multilayering and loss of epithelial integrity throughout the clones.Further analysis reveals that the chromosomes carrying the baz(4) and baz(815-8) alleles may contain additional mutations that enhance the true baz loss-of-function phenotype in the follicular epithelium.This study clearly shows that Baz is dispensable for the regulation of polarity in the follicular epithelium, and that the requirement for key regulators of cell polarity is highly dependent on developmental context and cell type.

View Article: PubMed Central - PubMed

Affiliation: Stammzellbiologie, Institut für Anatomie und Zellbiologie, Georg-August Universität Göttingen, Justus-von-Liebig-Weg 11, 37077 Göttingen, Germany.

No MeSH data available.


Related in: MedlinePlus

baz mutant cells lack Baz immunostaining.Ovarian follicles in which bazXR11 (A–A″″), bazEH747 (B–B″″), baz815-8 (C–C″″) and baz4 (D–D″″) mutant cells marked by loss of His-GFP were induced by hs-Flp mediated recombination. White boxes in A–D indicate regions shown in A′–A″″, B′–B″″, C′–C″″ and D–D″″, respectively. Mosaic follicles were immunostained with antibodies raised against amino acids 1-297 of the N-terminus of Baz (Baz N-term), amino acids 309-747 of the PDZ domain of Baz (Baz PDZ) and Dlg, which marks the basolateral cell boundaries as indicated. Both the Baz N-term and Baz PDZ antibodies show a complete absence of apical staining in bazXR11 (A″ and A′″), bazEH747 (B″–B′″), baz815-8 (C″–C′″) and baz4 (D″– D′″) mutant FE cells, respectively. Scale bars = 20 µm (A–D); 5 µm (all other scale bars).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4400595&req=5

f03: baz mutant cells lack Baz immunostaining.Ovarian follicles in which bazXR11 (A–A″″), bazEH747 (B–B″″), baz815-8 (C–C″″) and baz4 (D–D″″) mutant cells marked by loss of His-GFP were induced by hs-Flp mediated recombination. White boxes in A–D indicate regions shown in A′–A″″, B′–B″″, C′–C″″ and D–D″″, respectively. Mosaic follicles were immunostained with antibodies raised against amino acids 1-297 of the N-terminus of Baz (Baz N-term), amino acids 309-747 of the PDZ domain of Baz (Baz PDZ) and Dlg, which marks the basolateral cell boundaries as indicated. Both the Baz N-term and Baz PDZ antibodies show a complete absence of apical staining in bazXR11 (A″ and A′″), bazEH747 (B″–B′″), baz815-8 (C″–C′″) and baz4 (D″– D′″) mutant FE cells, respectively. Scale bars = 20 µm (A–D); 5 µm (all other scale bars).

Mentions: To confirm that the FE defects observed in baz4, baz815-8, bazEH747 and bazXR11 clones were indeed due to an absence of Baz, we conducted immunostaining analyses with an antibody that was raised against the Baz N-terminal domain and another antibody against the Baz Postsynaptic density 95/Dlg/Zonula occludens 1 (PDZ) domains. With both antibodies Baz staining in baz4, baz815-8, bazEH747 and bazXR11 FE clones was below the limit of detection (Fig. 3). Furthermore, all FE defects observed in baz4 (as previously reported by Benton and St Johnston, 2003; Morais-de-Sá et al., 2010) and bazEH747 clones were rescued by overexpression of full length UAS-Baz using the MARCM system (supplementary material Fig. S1).


Bazooka/PAR3 is dispensable for polarity in Drosophila follicular epithelial cells.

Shahab J, Tiwari MD, Honemann-Capito M, Krahn MP, Wodarz A - Biol Open (2015)

baz mutant cells lack Baz immunostaining.Ovarian follicles in which bazXR11 (A–A″″), bazEH747 (B–B″″), baz815-8 (C–C″″) and baz4 (D–D″″) mutant cells marked by loss of His-GFP were induced by hs-Flp mediated recombination. White boxes in A–D indicate regions shown in A′–A″″, B′–B″″, C′–C″″ and D–D″″, respectively. Mosaic follicles were immunostained with antibodies raised against amino acids 1-297 of the N-terminus of Baz (Baz N-term), amino acids 309-747 of the PDZ domain of Baz (Baz PDZ) and Dlg, which marks the basolateral cell boundaries as indicated. Both the Baz N-term and Baz PDZ antibodies show a complete absence of apical staining in bazXR11 (A″ and A′″), bazEH747 (B″–B′″), baz815-8 (C″–C′″) and baz4 (D″– D′″) mutant FE cells, respectively. Scale bars = 20 µm (A–D); 5 µm (all other scale bars).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400595&req=5

f03: baz mutant cells lack Baz immunostaining.Ovarian follicles in which bazXR11 (A–A″″), bazEH747 (B–B″″), baz815-8 (C–C″″) and baz4 (D–D″″) mutant cells marked by loss of His-GFP were induced by hs-Flp mediated recombination. White boxes in A–D indicate regions shown in A′–A″″, B′–B″″, C′–C″″ and D–D″″, respectively. Mosaic follicles were immunostained with antibodies raised against amino acids 1-297 of the N-terminus of Baz (Baz N-term), amino acids 309-747 of the PDZ domain of Baz (Baz PDZ) and Dlg, which marks the basolateral cell boundaries as indicated. Both the Baz N-term and Baz PDZ antibodies show a complete absence of apical staining in bazXR11 (A″ and A′″), bazEH747 (B″–B′″), baz815-8 (C″–C′″) and baz4 (D″– D′″) mutant FE cells, respectively. Scale bars = 20 µm (A–D); 5 µm (all other scale bars).
Mentions: To confirm that the FE defects observed in baz4, baz815-8, bazEH747 and bazXR11 clones were indeed due to an absence of Baz, we conducted immunostaining analyses with an antibody that was raised against the Baz N-terminal domain and another antibody against the Baz Postsynaptic density 95/Dlg/Zonula occludens 1 (PDZ) domains. With both antibodies Baz staining in baz4, baz815-8, bazEH747 and bazXR11 FE clones was below the limit of detection (Fig. 3). Furthermore, all FE defects observed in baz4 (as previously reported by Benton and St Johnston, 2003; Morais-de-Sá et al., 2010) and bazEH747 clones were rescued by overexpression of full length UAS-Baz using the MARCM system (supplementary material Fig. S1).

Bottom Line: While all these baz alleles display identical phenotypes during embryonic epithelial development, we observe strong discrepancies in the severity and penetrance of polarity defects in the follicular epithelium: polarity is mostly normal in baz(EH747) and baz(XR11) while baz(4) and baz(815) (-8) show loss of polarity, severe multilayering and loss of epithelial integrity throughout the clones.Further analysis reveals that the chromosomes carrying the baz(4) and baz(815-8) alleles may contain additional mutations that enhance the true baz loss-of-function phenotype in the follicular epithelium.This study clearly shows that Baz is dispensable for the regulation of polarity in the follicular epithelium, and that the requirement for key regulators of cell polarity is highly dependent on developmental context and cell type.

View Article: PubMed Central - PubMed

Affiliation: Stammzellbiologie, Institut für Anatomie und Zellbiologie, Georg-August Universität Göttingen, Justus-von-Liebig-Weg 11, 37077 Göttingen, Germany.

No MeSH data available.


Related in: MedlinePlus