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Bazooka/PAR3 is dispensable for polarity in Drosophila follicular epithelial cells.

Shahab J, Tiwari MD, Honemann-Capito M, Krahn MP, Wodarz A - Biol Open (2015)

Bottom Line: While all these baz alleles display identical phenotypes during embryonic epithelial development, we observe strong discrepancies in the severity and penetrance of polarity defects in the follicular epithelium: polarity is mostly normal in baz(EH747) and baz(XR11) while baz(4) and baz(815) (-8) show loss of polarity, severe multilayering and loss of epithelial integrity throughout the clones.Further analysis reveals that the chromosomes carrying the baz(4) and baz(815-8) alleles may contain additional mutations that enhance the true baz loss-of-function phenotype in the follicular epithelium.This study clearly shows that Baz is dispensable for the regulation of polarity in the follicular epithelium, and that the requirement for key regulators of cell polarity is highly dependent on developmental context and cell type.

View Article: PubMed Central - PubMed

Affiliation: Stammzellbiologie, Institut für Anatomie und Zellbiologie, Georg-August Universität Göttingen, Justus-von-Liebig-Weg 11, 37077 Göttingen, Germany.

No MeSH data available.


Related in: MedlinePlus

baz mutant posterior follicle cells show multilayering.Ovarian follicles in which bazXR11 (A–A′, stage 7), bazEH747 (B–B′, stage 7), baz815-8 (C-C′, stage 8) and baz4 (D–D′, stage 7) mutant cells marked by an absence of Ubi-GFP were generated using the directed mosaic system by expressing UAS-Flp under the control of e22c-gal4. White boxes in A,B,C,D indicate regions shown in A′,B′,C′,D′, respectively. Mosaic follicles were immunostained with GFP and stained with DAPI as indicated. PFCs mutant for bazXR11 (A′), bazEH747 (B′), baz815-8 (C′) and baz4 (D′) show multilayering as visualized by DAPI staining and indicated by arrows. (E) Bar graph presenting quantitative analysis of the PFC multilayering phenotype. The number of baz4, baz815-8, bazEH747, bazXR11, and FRT19A mosaic follicles with PFC multilayering were counted and presented as an average percentage from three repetitions of n = 50 (94% of baz4, 90% of baz815-8, 85% of bazXR11; 90% of bazEH747; 3% of FRT19A). At p<0.001, genotypes with same number of stars are not significantly different while those with different number of stars are significantly different. Error bars represent s.d. Scale bars = 20 µm (A–D); 5 µm (all other scale bars).
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f02: baz mutant posterior follicle cells show multilayering.Ovarian follicles in which bazXR11 (A–A′, stage 7), bazEH747 (B–B′, stage 7), baz815-8 (C-C′, stage 8) and baz4 (D–D′, stage 7) mutant cells marked by an absence of Ubi-GFP were generated using the directed mosaic system by expressing UAS-Flp under the control of e22c-gal4. White boxes in A,B,C,D indicate regions shown in A′,B′,C′,D′, respectively. Mosaic follicles were immunostained with GFP and stained with DAPI as indicated. PFCs mutant for bazXR11 (A′), bazEH747 (B′), baz815-8 (C′) and baz4 (D′) show multilayering as visualized by DAPI staining and indicated by arrows. (E) Bar graph presenting quantitative analysis of the PFC multilayering phenotype. The number of baz4, baz815-8, bazEH747, bazXR11, and FRT19A mosaic follicles with PFC multilayering were counted and presented as an average percentage from three repetitions of n = 50 (94% of baz4, 90% of baz815-8, 85% of bazXR11; 90% of bazEH747; 3% of FRT19A). At p<0.001, genotypes with same number of stars are not significantly different while those with different number of stars are significantly different. Error bars represent s.d. Scale bars = 20 µm (A–D); 5 µm (all other scale bars).

Mentions: It was previously reported that large baz mutant clones positioned along the lateral FE lose integrity, which results in the formation of gaps and discontinuities, thus exposing the underlying nurse cells. On the other hand, baz PFC mutant clones were reported to show strong multilayering (Abdelilah-Seyfried et al., 2003). Our results confirmed these findings with regards to baz4 and baz815-8 FE clones which often form large holes in the lateral FE and display strong multilayering of the PFCs (Figs 1 and 2). On the contrary, large holes or discontinuities were rarely observed in bazEH747 and bazXR11 lateral FE clones (Fig. 1), whereas careful examination of PFC clones revealed a mild, yet highly penetrant multilayering phenotype (Fig. 2). In contrast to PFC clones for baz4 and baz815-8, which show severe multilayering throughout the clone, multilayering in bazEH747 and bazXR11 PFC clones is normally restricted to only a few cells (Fig. 2).


Bazooka/PAR3 is dispensable for polarity in Drosophila follicular epithelial cells.

Shahab J, Tiwari MD, Honemann-Capito M, Krahn MP, Wodarz A - Biol Open (2015)

baz mutant posterior follicle cells show multilayering.Ovarian follicles in which bazXR11 (A–A′, stage 7), bazEH747 (B–B′, stage 7), baz815-8 (C-C′, stage 8) and baz4 (D–D′, stage 7) mutant cells marked by an absence of Ubi-GFP were generated using the directed mosaic system by expressing UAS-Flp under the control of e22c-gal4. White boxes in A,B,C,D indicate regions shown in A′,B′,C′,D′, respectively. Mosaic follicles were immunostained with GFP and stained with DAPI as indicated. PFCs mutant for bazXR11 (A′), bazEH747 (B′), baz815-8 (C′) and baz4 (D′) show multilayering as visualized by DAPI staining and indicated by arrows. (E) Bar graph presenting quantitative analysis of the PFC multilayering phenotype. The number of baz4, baz815-8, bazEH747, bazXR11, and FRT19A mosaic follicles with PFC multilayering were counted and presented as an average percentage from three repetitions of n = 50 (94% of baz4, 90% of baz815-8, 85% of bazXR11; 90% of bazEH747; 3% of FRT19A). At p<0.001, genotypes with same number of stars are not significantly different while those with different number of stars are significantly different. Error bars represent s.d. Scale bars = 20 µm (A–D); 5 µm (all other scale bars).
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Related In: Results  -  Collection

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f02: baz mutant posterior follicle cells show multilayering.Ovarian follicles in which bazXR11 (A–A′, stage 7), bazEH747 (B–B′, stage 7), baz815-8 (C-C′, stage 8) and baz4 (D–D′, stage 7) mutant cells marked by an absence of Ubi-GFP were generated using the directed mosaic system by expressing UAS-Flp under the control of e22c-gal4. White boxes in A,B,C,D indicate regions shown in A′,B′,C′,D′, respectively. Mosaic follicles were immunostained with GFP and stained with DAPI as indicated. PFCs mutant for bazXR11 (A′), bazEH747 (B′), baz815-8 (C′) and baz4 (D′) show multilayering as visualized by DAPI staining and indicated by arrows. (E) Bar graph presenting quantitative analysis of the PFC multilayering phenotype. The number of baz4, baz815-8, bazEH747, bazXR11, and FRT19A mosaic follicles with PFC multilayering were counted and presented as an average percentage from three repetitions of n = 50 (94% of baz4, 90% of baz815-8, 85% of bazXR11; 90% of bazEH747; 3% of FRT19A). At p<0.001, genotypes with same number of stars are not significantly different while those with different number of stars are significantly different. Error bars represent s.d. Scale bars = 20 µm (A–D); 5 µm (all other scale bars).
Mentions: It was previously reported that large baz mutant clones positioned along the lateral FE lose integrity, which results in the formation of gaps and discontinuities, thus exposing the underlying nurse cells. On the other hand, baz PFC mutant clones were reported to show strong multilayering (Abdelilah-Seyfried et al., 2003). Our results confirmed these findings with regards to baz4 and baz815-8 FE clones which often form large holes in the lateral FE and display strong multilayering of the PFCs (Figs 1 and 2). On the contrary, large holes or discontinuities were rarely observed in bazEH747 and bazXR11 lateral FE clones (Fig. 1), whereas careful examination of PFC clones revealed a mild, yet highly penetrant multilayering phenotype (Fig. 2). In contrast to PFC clones for baz4 and baz815-8, which show severe multilayering throughout the clone, multilayering in bazEH747 and bazXR11 PFC clones is normally restricted to only a few cells (Fig. 2).

Bottom Line: While all these baz alleles display identical phenotypes during embryonic epithelial development, we observe strong discrepancies in the severity and penetrance of polarity defects in the follicular epithelium: polarity is mostly normal in baz(EH747) and baz(XR11) while baz(4) and baz(815) (-8) show loss of polarity, severe multilayering and loss of epithelial integrity throughout the clones.Further analysis reveals that the chromosomes carrying the baz(4) and baz(815-8) alleles may contain additional mutations that enhance the true baz loss-of-function phenotype in the follicular epithelium.This study clearly shows that Baz is dispensable for the regulation of polarity in the follicular epithelium, and that the requirement for key regulators of cell polarity is highly dependent on developmental context and cell type.

View Article: PubMed Central - PubMed

Affiliation: Stammzellbiologie, Institut für Anatomie und Zellbiologie, Georg-August Universität Göttingen, Justus-von-Liebig-Weg 11, 37077 Göttingen, Germany.

No MeSH data available.


Related in: MedlinePlus