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Aberrant over-expression of TRPM7 ion channels in pancreatic cancer: required for cancer cell invasion and implicated in tumor growth and metastasis.

Yee NS, Kazi AA, Li Q, Yang Z, Berg A, Yee RK - Biol Open (2015)

Bottom Line: Anti-TRPM7 immunoreactivity in pancreatic adenocarcinoma significantly correlates with the size and stages of tumors.In human pancreatic adenocarcinoma cells in which TRPM7 is highly expressed, short hairpin RNA-mediated suppression of TRPM7 impairs cell invasion.The results demonstrate that TRPM7 channels are over-expressed in a proportion of the pre-malignant lesions and malignant tumors of the pancreas, and they are necessary for invasion by pancreatic cancer cells.

View Article: PubMed Central - PubMed

Affiliation: Division of Hematology-Oncology, Department of Medicine, Penn State College of Medicine, Program of Experimental Therapeutics, Penn State Hershey Cancer Institute, Penn State Milton S. Hershey Medical Center, Pennsylvania State University, Hershey, PA 17033, USA nyee@hmc.psu.edu.

No MeSH data available.


Related in: MedlinePlus

Immunohistochemical analysis of TRPM7 in non-malignant pancreatic tissues.(A) Normal pancreatic tissues. (B) Chronic pancreatitis. (C) Pancreatic intra-epithelial neoplasm. (D) Intraductal papillary mucinous neoplasm. Original magnification ×400. In panel A, yellow arrows point at ducts, and pink arrows point at centroacinar cells; the islet is surrounded with red dotted lines.
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f01: Immunohistochemical analysis of TRPM7 in non-malignant pancreatic tissues.(A) Normal pancreatic tissues. (B) Chronic pancreatitis. (C) Pancreatic intra-epithelial neoplasm. (D) Intraductal papillary mucinous neoplasm. Original magnification ×400. In panel A, yellow arrows point at ducts, and pink arrows point at centroacinar cells; the islet is surrounded with red dotted lines.

Mentions: In the normal tissues of exocrine pancreas, anti-TRPM7 immunoreactivity was observed in the ductal and centroacinar cells, but minimal, if any, in the acinar cells (Fig. 1A). In the ductal epithelia and centroacinar cells, expression of TRPM7 is relatively low and mostly cytoplasmic with accentuation on the luminal plasma membrane. In the endocrine cells of the pancreatic islets, there is a moderate level of anti-TRPM7 immunoreactivity with uneven distribution in the cytoplasm (Fig. 1A). In chronic pancreatitis, the expression level of TRPM7 is relatively high; anti-TRPM7 immunoreactivity in the cytoplasm of the pancreatic ductal and acinar cells is readily detectable (Fig. 1B). In pancreatic intra-epithelial neoplasms (PanINs) and intraductal papillary mucinous neoplasm (IPMN), there is a moderate level of TRPM7 expression that appears to be localized on the plasma membrane (Fig. 1C,D). Thus, TRPM7 is differentially expressed in different types of epithelia in normal pancreatic tissues, and its expression appears elevated in chronically inflamed and pre-malignant tissues with localization on the plasma membrane.


Aberrant over-expression of TRPM7 ion channels in pancreatic cancer: required for cancer cell invasion and implicated in tumor growth and metastasis.

Yee NS, Kazi AA, Li Q, Yang Z, Berg A, Yee RK - Biol Open (2015)

Immunohistochemical analysis of TRPM7 in non-malignant pancreatic tissues.(A) Normal pancreatic tissues. (B) Chronic pancreatitis. (C) Pancreatic intra-epithelial neoplasm. (D) Intraductal papillary mucinous neoplasm. Original magnification ×400. In panel A, yellow arrows point at ducts, and pink arrows point at centroacinar cells; the islet is surrounded with red dotted lines.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400593&req=5

f01: Immunohistochemical analysis of TRPM7 in non-malignant pancreatic tissues.(A) Normal pancreatic tissues. (B) Chronic pancreatitis. (C) Pancreatic intra-epithelial neoplasm. (D) Intraductal papillary mucinous neoplasm. Original magnification ×400. In panel A, yellow arrows point at ducts, and pink arrows point at centroacinar cells; the islet is surrounded with red dotted lines.
Mentions: In the normal tissues of exocrine pancreas, anti-TRPM7 immunoreactivity was observed in the ductal and centroacinar cells, but minimal, if any, in the acinar cells (Fig. 1A). In the ductal epithelia and centroacinar cells, expression of TRPM7 is relatively low and mostly cytoplasmic with accentuation on the luminal plasma membrane. In the endocrine cells of the pancreatic islets, there is a moderate level of anti-TRPM7 immunoreactivity with uneven distribution in the cytoplasm (Fig. 1A). In chronic pancreatitis, the expression level of TRPM7 is relatively high; anti-TRPM7 immunoreactivity in the cytoplasm of the pancreatic ductal and acinar cells is readily detectable (Fig. 1B). In pancreatic intra-epithelial neoplasms (PanINs) and intraductal papillary mucinous neoplasm (IPMN), there is a moderate level of TRPM7 expression that appears to be localized on the plasma membrane (Fig. 1C,D). Thus, TRPM7 is differentially expressed in different types of epithelia in normal pancreatic tissues, and its expression appears elevated in chronically inflamed and pre-malignant tissues with localization on the plasma membrane.

Bottom Line: Anti-TRPM7 immunoreactivity in pancreatic adenocarcinoma significantly correlates with the size and stages of tumors.In human pancreatic adenocarcinoma cells in which TRPM7 is highly expressed, short hairpin RNA-mediated suppression of TRPM7 impairs cell invasion.The results demonstrate that TRPM7 channels are over-expressed in a proportion of the pre-malignant lesions and malignant tumors of the pancreas, and they are necessary for invasion by pancreatic cancer cells.

View Article: PubMed Central - PubMed

Affiliation: Division of Hematology-Oncology, Department of Medicine, Penn State College of Medicine, Program of Experimental Therapeutics, Penn State Hershey Cancer Institute, Penn State Milton S. Hershey Medical Center, Pennsylvania State University, Hershey, PA 17033, USA nyee@hmc.psu.edu.

No MeSH data available.


Related in: MedlinePlus