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Differences in the fatty-acid composition of rodent spermatozoa are associated to levels of sperm competition.

delBarco-Trillo J, Mateo R, Roldan ER - Biol Open (2015)

Bottom Line: To test these predictions, we quantified the fatty-acid composition of sperm, testis and liver cells in four mouse species (genus Mus) that differ in their levels of sperm competition.Fatty-acid composition in testis and liver cells was not associated to sperm competition levels.Two particular fatty acids were most responsible for this pattern (arachidonic acid and palmitic acid).

View Article: PubMed Central - PubMed

Affiliation: Reproductive Ecology and Biology Group, Museo Nacional de Ciencias Naturales, CSIC, 28006 Madrid, Spain Present address: School of Natural Sciences and Psychology, Liverpool John Moores University, Liverpool L3 5UA, UK. delbarcotrillo@gmail.com.

No MeSH data available.


Related in: MedlinePlus

ShRNA-mediated silencing of TRPM7 impaired invasion in pancreatic adenocarcinoma cells.(A) Non-transfected BxPC-3 cells assayed for invasion using the trans-well assay in the presence of 3% FBS or no FBS. (B,C) BxPC-3 cells transfected with non-targeting control (NC) shRNA or anti-TRPM7 shRNA analyzed for cell invasion in the presence of either 3% FBS (B) or 10% FBS (C). A representative image of the invaded cells stained with crystal violet is shown for each experimental group. Cell invasion is expressed as % control, and each column represents the mean ± standard error.
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f06: ShRNA-mediated silencing of TRPM7 impaired invasion in pancreatic adenocarcinoma cells.(A) Non-transfected BxPC-3 cells assayed for invasion using the trans-well assay in the presence of 3% FBS or no FBS. (B,C) BxPC-3 cells transfected with non-targeting control (NC) shRNA or anti-TRPM7 shRNA analyzed for cell invasion in the presence of either 3% FBS (B) or 10% FBS (C). A representative image of the invaded cells stained with crystal violet is shown for each experimental group. Cell invasion is expressed as % control, and each column represents the mean ± standard error.


Differences in the fatty-acid composition of rodent spermatozoa are associated to levels of sperm competition.

delBarco-Trillo J, Mateo R, Roldan ER - Biol Open (2015)

ShRNA-mediated silencing of TRPM7 impaired invasion in pancreatic adenocarcinoma cells.(A) Non-transfected BxPC-3 cells assayed for invasion using the trans-well assay in the presence of 3% FBS or no FBS. (B,C) BxPC-3 cells transfected with non-targeting control (NC) shRNA or anti-TRPM7 shRNA analyzed for cell invasion in the presence of either 3% FBS (B) or 10% FBS (C). A representative image of the invaded cells stained with crystal violet is shown for each experimental group. Cell invasion is expressed as % control, and each column represents the mean ± standard error.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400589&req=5

f06: ShRNA-mediated silencing of TRPM7 impaired invasion in pancreatic adenocarcinoma cells.(A) Non-transfected BxPC-3 cells assayed for invasion using the trans-well assay in the presence of 3% FBS or no FBS. (B,C) BxPC-3 cells transfected with non-targeting control (NC) shRNA or anti-TRPM7 shRNA analyzed for cell invasion in the presence of either 3% FBS (B) or 10% FBS (C). A representative image of the invaded cells stained with crystal violet is shown for each experimental group. Cell invasion is expressed as % control, and each column represents the mean ± standard error.
Bottom Line: To test these predictions, we quantified the fatty-acid composition of sperm, testis and liver cells in four mouse species (genus Mus) that differ in their levels of sperm competition.Fatty-acid composition in testis and liver cells was not associated to sperm competition levels.Two particular fatty acids were most responsible for this pattern (arachidonic acid and palmitic acid).

View Article: PubMed Central - PubMed

Affiliation: Reproductive Ecology and Biology Group, Museo Nacional de Ciencias Naturales, CSIC, 28006 Madrid, Spain Present address: School of Natural Sciences and Psychology, Liverpool John Moores University, Liverpool L3 5UA, UK. delbarcotrillo@gmail.com.

No MeSH data available.


Related in: MedlinePlus