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Differences in the fatty-acid composition of rodent spermatozoa are associated to levels of sperm competition.

delBarco-Trillo J, Mateo R, Roldan ER - Biol Open (2015)

Bottom Line: To test these predictions, we quantified the fatty-acid composition of sperm, testis and liver cells in four mouse species (genus Mus) that differ in their levels of sperm competition.Fatty-acid composition in testis and liver cells was not associated to sperm competition levels.Two particular fatty acids were most responsible for this pattern (arachidonic acid and palmitic acid).

View Article: PubMed Central - PubMed

Affiliation: Reproductive Ecology and Biology Group, Museo Nacional de Ciencias Naturales, CSIC, 28006 Madrid, Spain Present address: School of Natural Sciences and Psychology, Liverpool John Moores University, Liverpool L3 5UA, UK. delbarcotrillo@gmail.com.

No MeSH data available.


Related in: MedlinePlus

TRPM7 is over-expressed in pancreatic adenocarcinoma cells, and its expression can be down-regulated by shRNA.(A) Cell lysates of each cell line were analyzed for the protein levels of TRPM7 by immunoblotting. The relative amount of TRPM7 and GAPDH protein in each cancer cell line was compared to that in H6c7 as indicated (mean ± standard error). (B) Immunoblotting analysis of TRPM7 protein in BxPC-3 cells transfected with either non-targeting control (NC) shRNA or anti-TRPM7 shRNA. The relative amount of TRPM7 and GAPDH protein in each group of cells is expressed as % of control (NC shRNA). Each column represents the mean ± standard error of three experiments with duplicate samples in each experiment. *indicates statistically significant difference with P<0.05.
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f05: TRPM7 is over-expressed in pancreatic adenocarcinoma cells, and its expression can be down-regulated by shRNA.(A) Cell lysates of each cell line were analyzed for the protein levels of TRPM7 by immunoblotting. The relative amount of TRPM7 and GAPDH protein in each cancer cell line was compared to that in H6c7 as indicated (mean ± standard error). (B) Immunoblotting analysis of TRPM7 protein in BxPC-3 cells transfected with either non-targeting control (NC) shRNA or anti-TRPM7 shRNA. The relative amount of TRPM7 and GAPDH protein in each group of cells is expressed as % of control (NC shRNA). Each column represents the mean ± standard error of three experiments with duplicate samples in each experiment. *indicates statistically significant difference with P<0.05.


Differences in the fatty-acid composition of rodent spermatozoa are associated to levels of sperm competition.

delBarco-Trillo J, Mateo R, Roldan ER - Biol Open (2015)

TRPM7 is over-expressed in pancreatic adenocarcinoma cells, and its expression can be down-regulated by shRNA.(A) Cell lysates of each cell line were analyzed for the protein levels of TRPM7 by immunoblotting. The relative amount of TRPM7 and GAPDH protein in each cancer cell line was compared to that in H6c7 as indicated (mean ± standard error). (B) Immunoblotting analysis of TRPM7 protein in BxPC-3 cells transfected with either non-targeting control (NC) shRNA or anti-TRPM7 shRNA. The relative amount of TRPM7 and GAPDH protein in each group of cells is expressed as % of control (NC shRNA). Each column represents the mean ± standard error of three experiments with duplicate samples in each experiment. *indicates statistically significant difference with P<0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400589&req=5

f05: TRPM7 is over-expressed in pancreatic adenocarcinoma cells, and its expression can be down-regulated by shRNA.(A) Cell lysates of each cell line were analyzed for the protein levels of TRPM7 by immunoblotting. The relative amount of TRPM7 and GAPDH protein in each cancer cell line was compared to that in H6c7 as indicated (mean ± standard error). (B) Immunoblotting analysis of TRPM7 protein in BxPC-3 cells transfected with either non-targeting control (NC) shRNA or anti-TRPM7 shRNA. The relative amount of TRPM7 and GAPDH protein in each group of cells is expressed as % of control (NC shRNA). Each column represents the mean ± standard error of three experiments with duplicate samples in each experiment. *indicates statistically significant difference with P<0.05.
Bottom Line: To test these predictions, we quantified the fatty-acid composition of sperm, testis and liver cells in four mouse species (genus Mus) that differ in their levels of sperm competition.Fatty-acid composition in testis and liver cells was not associated to sperm competition levels.Two particular fatty acids were most responsible for this pattern (arachidonic acid and palmitic acid).

View Article: PubMed Central - PubMed

Affiliation: Reproductive Ecology and Biology Group, Museo Nacional de Ciencias Naturales, CSIC, 28006 Madrid, Spain Present address: School of Natural Sciences and Psychology, Liverpool John Moores University, Liverpool L3 5UA, UK. delbarcotrillo@gmail.com.

No MeSH data available.


Related in: MedlinePlus