Limits...
Evidence for fungal infection in cerebrospinal fluid and brain tissue from patients with amyotrophic lateral sclerosis.

Alonso R, Pisa D, Marina AI, Morato E, Rábano A, Rodal I, Carrasco L - Int. J. Biol. Sci. (2015)

Bottom Line: ALS is the most common form of motor neuron disease; yet, to date, the exact etiology of ALS remains unknown.Fungal antigens, as well as DNA from several fungi, were detected in CSF from ALS patients.Additionally, examination of brain sections from the frontal cortex of ALS patients revealed the existence of immunopositive fungal antigens comprising punctate bodies in the cytoplasm of some neurons.

View Article: PubMed Central - PubMed

Affiliation: 1. Centro de Biología Molecular "Severo Ochoa". c/Nicolás Cabrera, 1. Universidad Autónoma de Madrid. Cantoblanco. 28049 Madrid. Spain.

ABSTRACT
Among neurogenerative diseases, amyotrophic lateral sclerosis (ALS) is a fatal illness characterized by a progressive motor neuron dysfunction in the motor cortex, brainstem and spinal cord. ALS is the most common form of motor neuron disease; yet, to date, the exact etiology of ALS remains unknown. In the present work, we have explored the possibility of fungal infection in cerebrospinal fluid (CSF) and in brain tissue from ALS patients. Fungal antigens, as well as DNA from several fungi, were detected in CSF from ALS patients. Additionally, examination of brain sections from the frontal cortex of ALS patients revealed the existence of immunopositive fungal antigens comprising punctate bodies in the cytoplasm of some neurons. Fungal DNA was also detected in brain tissue using PCR analysis, uncovering the presence of several fungal species. Finally, proteomic analyses of brain tissue demonstrated the occurrence of several fungal peptides. Collectively, our observations provide compelling evidence of fungal infection in the ALS patients analyzed, suggesting that this infection may play a part in the etiology of the disease or may constitute a risk factor for these patients.

Show MeSH

Related in: MedlinePlus

Functional grouping of human proteins specifically present in the frontal cortex from ALS patients. Human proteins which are common in the three ALS patients that are not present in control brain grouped by: a) cellular component; b) molecular function; c) protein class.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4400386&req=5

Figure 6: Functional grouping of human proteins specifically present in the frontal cortex from ALS patients. Human proteins which are common in the three ALS patients that are not present in control brain grouped by: a) cellular component; b) molecular function; c) protein class.

Mentions: The human proteins common to the three patients that were not present in a control recently published by our laboratory are listed in Supplementary Table SIII. These proteins can be grouped according to their cellular function (Figure 6). These proteins may constitute the basis for future ALS biomarkers.


Evidence for fungal infection in cerebrospinal fluid and brain tissue from patients with amyotrophic lateral sclerosis.

Alonso R, Pisa D, Marina AI, Morato E, Rábano A, Rodal I, Carrasco L - Int. J. Biol. Sci. (2015)

Functional grouping of human proteins specifically present in the frontal cortex from ALS patients. Human proteins which are common in the three ALS patients that are not present in control brain grouped by: a) cellular component; b) molecular function; c) protein class.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4400386&req=5

Figure 6: Functional grouping of human proteins specifically present in the frontal cortex from ALS patients. Human proteins which are common in the three ALS patients that are not present in control brain grouped by: a) cellular component; b) molecular function; c) protein class.
Mentions: The human proteins common to the three patients that were not present in a control recently published by our laboratory are listed in Supplementary Table SIII. These proteins can be grouped according to their cellular function (Figure 6). These proteins may constitute the basis for future ALS biomarkers.

Bottom Line: ALS is the most common form of motor neuron disease; yet, to date, the exact etiology of ALS remains unknown.Fungal antigens, as well as DNA from several fungi, were detected in CSF from ALS patients.Additionally, examination of brain sections from the frontal cortex of ALS patients revealed the existence of immunopositive fungal antigens comprising punctate bodies in the cytoplasm of some neurons.

View Article: PubMed Central - PubMed

Affiliation: 1. Centro de Biología Molecular "Severo Ochoa". c/Nicolás Cabrera, 1. Universidad Autónoma de Madrid. Cantoblanco. 28049 Madrid. Spain.

ABSTRACT
Among neurogenerative diseases, amyotrophic lateral sclerosis (ALS) is a fatal illness characterized by a progressive motor neuron dysfunction in the motor cortex, brainstem and spinal cord. ALS is the most common form of motor neuron disease; yet, to date, the exact etiology of ALS remains unknown. In the present work, we have explored the possibility of fungal infection in cerebrospinal fluid (CSF) and in brain tissue from ALS patients. Fungal antigens, as well as DNA from several fungi, were detected in CSF from ALS patients. Additionally, examination of brain sections from the frontal cortex of ALS patients revealed the existence of immunopositive fungal antigens comprising punctate bodies in the cytoplasm of some neurons. Fungal DNA was also detected in brain tissue using PCR analysis, uncovering the presence of several fungal species. Finally, proteomic analyses of brain tissue demonstrated the occurrence of several fungal peptides. Collectively, our observations provide compelling evidence of fungal infection in the ALS patients analyzed, suggesting that this infection may play a part in the etiology of the disease or may constitute a risk factor for these patients.

Show MeSH
Related in: MedlinePlus