Limits...
Sul1 and Sul2 sulfate transceptors signal to protein kinase A upon exit of sulfur starvation.

Kankipati HN, Rubio-Texeira M, Castermans D, Diallinas G, Thevelein JM - J. Biol. Chem. (2015)

Bottom Line: Overall, our data suggest that transceptors can undergo independent conformational changes, each responsible for triggering different downstream processes.The Sul1 and Sul2 transceptors are the first identified plasma membrane sensors for extracellular sulfate.High affinity transporters induced upon starvation for their substrate may generally act as transceptors during exit from starvation.

View Article: PubMed Central - PubMed

Affiliation: From the Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, KU Leuven, Kasteelpark Arenberg 31, B-3001 Leuven-Heverlee, Flanders, Belgium, the Department of Molecular Microbiology, VIB, Kasteelpark Arenberg 31, B-3001 Leuven-Heverlee, Flanders, Belgium, and.

Show MeSH

Related in: MedlinePlus

Cell growth with different sulfate-containing compounds as sole source of sulfur. Wild type (●), sul1Δ (○), sul2Δ (■), and sul1Δ sul2Δ (□) cells were starved for sulfur and then transferred to medium containing a 3 mm concentration of the indicated sulfate-containing compounds (A–D).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4400352&req=5

Figure 3: Cell growth with different sulfate-containing compounds as sole source of sulfur. Wild type (●), sul1Δ (○), sul2Δ (■), and sul1Δ sul2Δ (□) cells were starved for sulfur and then transferred to medium containing a 3 mm concentration of the indicated sulfate-containing compounds (A–D).

Mentions: We also investigated whether the sulfate analogues could be used as a sole source of sulfur by yeast cells. We found that wild type (and also sul1Δ and sul2Δ single deletion) strains, but not the sul1Δ sul2Δ strain, were able to grow using methyl sulfate and 2-aminoethyl hydrogen sulfate as the sole source of sulfur. This indicated that methyl sulfate and 2-aminoethyl hydrogen sulfate were apparently transported into the cells by Sul1 and Sul2 and also further metabolized to support growth. On the other hand, d-glucosamine 2-sulfate did not support growth of any of the strains tested (Table 3 and Fig. 3). This is consistent with the observation that d-glucosamine 2-sulfate was not taken up by yeast cells. In order to rule out that growth in methyl sulfate and 2-aminoethyl hydrogen sulfate was due to release of sulfate from these compounds in the medium by secreted sulfatases, we deleted the only known sulfatase gene, BDS1, present in S. cerevisiae (39). The bds1Δ strain showed similar growth in methyl sulfate and 2-aminoethyl hydrogen sulfate as sole sources of sulfur as the wild type strain (Fig. 4), indicating that growth was not due to extracellular release of sulfate from these compounds but rather to uptake and intracellular assimilation.


Sul1 and Sul2 sulfate transceptors signal to protein kinase A upon exit of sulfur starvation.

Kankipati HN, Rubio-Texeira M, Castermans D, Diallinas G, Thevelein JM - J. Biol. Chem. (2015)

Cell growth with different sulfate-containing compounds as sole source of sulfur. Wild type (●), sul1Δ (○), sul2Δ (■), and sul1Δ sul2Δ (□) cells were starved for sulfur and then transferred to medium containing a 3 mm concentration of the indicated sulfate-containing compounds (A–D).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400352&req=5

Figure 3: Cell growth with different sulfate-containing compounds as sole source of sulfur. Wild type (●), sul1Δ (○), sul2Δ (■), and sul1Δ sul2Δ (□) cells were starved for sulfur and then transferred to medium containing a 3 mm concentration of the indicated sulfate-containing compounds (A–D).
Mentions: We also investigated whether the sulfate analogues could be used as a sole source of sulfur by yeast cells. We found that wild type (and also sul1Δ and sul2Δ single deletion) strains, but not the sul1Δ sul2Δ strain, were able to grow using methyl sulfate and 2-aminoethyl hydrogen sulfate as the sole source of sulfur. This indicated that methyl sulfate and 2-aminoethyl hydrogen sulfate were apparently transported into the cells by Sul1 and Sul2 and also further metabolized to support growth. On the other hand, d-glucosamine 2-sulfate did not support growth of any of the strains tested (Table 3 and Fig. 3). This is consistent with the observation that d-glucosamine 2-sulfate was not taken up by yeast cells. In order to rule out that growth in methyl sulfate and 2-aminoethyl hydrogen sulfate was due to release of sulfate from these compounds in the medium by secreted sulfatases, we deleted the only known sulfatase gene, BDS1, present in S. cerevisiae (39). The bds1Δ strain showed similar growth in methyl sulfate and 2-aminoethyl hydrogen sulfate as sole sources of sulfur as the wild type strain (Fig. 4), indicating that growth was not due to extracellular release of sulfate from these compounds but rather to uptake and intracellular assimilation.

Bottom Line: Overall, our data suggest that transceptors can undergo independent conformational changes, each responsible for triggering different downstream processes.The Sul1 and Sul2 transceptors are the first identified plasma membrane sensors for extracellular sulfate.High affinity transporters induced upon starvation for their substrate may generally act as transceptors during exit from starvation.

View Article: PubMed Central - PubMed

Affiliation: From the Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, KU Leuven, Kasteelpark Arenberg 31, B-3001 Leuven-Heverlee, Flanders, Belgium, the Department of Molecular Microbiology, VIB, Kasteelpark Arenberg 31, B-3001 Leuven-Heverlee, Flanders, Belgium, and.

Show MeSH
Related in: MedlinePlus