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Sul1 and Sul2 sulfate transceptors signal to protein kinase A upon exit of sulfur starvation.

Kankipati HN, Rubio-Texeira M, Castermans D, Diallinas G, Thevelein JM - J. Biol. Chem. (2015)

Bottom Line: Overall, our data suggest that transceptors can undergo independent conformational changes, each responsible for triggering different downstream processes.The Sul1 and Sul2 transceptors are the first identified plasma membrane sensors for extracellular sulfate.High affinity transporters induced upon starvation for their substrate may generally act as transceptors during exit from starvation.

View Article: PubMed Central - PubMed

Affiliation: From the Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, KU Leuven, Kasteelpark Arenberg 31, B-3001 Leuven-Heverlee, Flanders, Belgium, the Department of Molecular Microbiology, VIB, Kasteelpark Arenberg 31, B-3001 Leuven-Heverlee, Flanders, Belgium, and.

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d-Glucosamine 2-sulfate activates the PKA target trehalase, whereas its uptake is below the detection level.A, activation of trehalase in sulfur-starved wild type (●), sul1Δ (○), sul2Δ (▴), and sul1Δ sul2Δ (▵) cells after the addition of 3 mmd-glucosamine 2-sulfate. B, relative expression of HSP12 in sulfur-starved sul1Δ sul2Δ cells expressing Sul1-HA (circles) and Sul2-HA (triangles) upon the addition of 3 mm sulfate (closed symbols) or 3 mmd-glucosamine 2-sulfate (open symbols). C, uptake rate of 3H-labeled d-glucosamine 2-sulfate and 35S-labeled sodium sulfate in wild type (black bars) and sul1Δ sul2Δ (white bars). D, uptake rate of different concentrations of 35S-labeled sodium sulfate in the presence (black bars) and absence (white bars) of 10 mmd-glucosamine 2-sulfate (G2S). Error bars, S.D.
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Figure 2: d-Glucosamine 2-sulfate activates the PKA target trehalase, whereas its uptake is below the detection level.A, activation of trehalase in sulfur-starved wild type (●), sul1Δ (○), sul2Δ (▴), and sul1Δ sul2Δ (▵) cells after the addition of 3 mmd-glucosamine 2-sulfate. B, relative expression of HSP12 in sulfur-starved sul1Δ sul2Δ cells expressing Sul1-HA (circles) and Sul2-HA (triangles) upon the addition of 3 mm sulfate (closed symbols) or 3 mmd-glucosamine 2-sulfate (open symbols). C, uptake rate of 3H-labeled d-glucosamine 2-sulfate and 35S-labeled sodium sulfate in wild type (black bars) and sul1Δ sul2Δ (white bars). D, uptake rate of different concentrations of 35S-labeled sodium sulfate in the presence (black bars) and absence (white bars) of 10 mmd-glucosamine 2-sulfate (G2S). Error bars, S.D.

Mentions: We found three compounds able to activate trehalase in a Sul1- or Sul2-dependent manner: methyl sulfate, 2-aminoethyl hydrogen sulfate (Table 3), and d-glucosamine 2-sulfate (Fig. 2A). To confirm the Sul1/Sul2-dependent signaling nature of d-glucosamine 2-sulfate, we measured the gene expression level of HSP12 and show that it drops upon the addition of 3 mm sulfate or 3 mmd-glucosamine 2-sulfate in cells only expressing Sul1-HA or Sul2-HA (Fig. 2B).


Sul1 and Sul2 sulfate transceptors signal to protein kinase A upon exit of sulfur starvation.

Kankipati HN, Rubio-Texeira M, Castermans D, Diallinas G, Thevelein JM - J. Biol. Chem. (2015)

d-Glucosamine 2-sulfate activates the PKA target trehalase, whereas its uptake is below the detection level.A, activation of trehalase in sulfur-starved wild type (●), sul1Δ (○), sul2Δ (▴), and sul1Δ sul2Δ (▵) cells after the addition of 3 mmd-glucosamine 2-sulfate. B, relative expression of HSP12 in sulfur-starved sul1Δ sul2Δ cells expressing Sul1-HA (circles) and Sul2-HA (triangles) upon the addition of 3 mm sulfate (closed symbols) or 3 mmd-glucosamine 2-sulfate (open symbols). C, uptake rate of 3H-labeled d-glucosamine 2-sulfate and 35S-labeled sodium sulfate in wild type (black bars) and sul1Δ sul2Δ (white bars). D, uptake rate of different concentrations of 35S-labeled sodium sulfate in the presence (black bars) and absence (white bars) of 10 mmd-glucosamine 2-sulfate (G2S). Error bars, S.D.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400352&req=5

Figure 2: d-Glucosamine 2-sulfate activates the PKA target trehalase, whereas its uptake is below the detection level.A, activation of trehalase in sulfur-starved wild type (●), sul1Δ (○), sul2Δ (▴), and sul1Δ sul2Δ (▵) cells after the addition of 3 mmd-glucosamine 2-sulfate. B, relative expression of HSP12 in sulfur-starved sul1Δ sul2Δ cells expressing Sul1-HA (circles) and Sul2-HA (triangles) upon the addition of 3 mm sulfate (closed symbols) or 3 mmd-glucosamine 2-sulfate (open symbols). C, uptake rate of 3H-labeled d-glucosamine 2-sulfate and 35S-labeled sodium sulfate in wild type (black bars) and sul1Δ sul2Δ (white bars). D, uptake rate of different concentrations of 35S-labeled sodium sulfate in the presence (black bars) and absence (white bars) of 10 mmd-glucosamine 2-sulfate (G2S). Error bars, S.D.
Mentions: We found three compounds able to activate trehalase in a Sul1- or Sul2-dependent manner: methyl sulfate, 2-aminoethyl hydrogen sulfate (Table 3), and d-glucosamine 2-sulfate (Fig. 2A). To confirm the Sul1/Sul2-dependent signaling nature of d-glucosamine 2-sulfate, we measured the gene expression level of HSP12 and show that it drops upon the addition of 3 mm sulfate or 3 mmd-glucosamine 2-sulfate in cells only expressing Sul1-HA or Sul2-HA (Fig. 2B).

Bottom Line: Overall, our data suggest that transceptors can undergo independent conformational changes, each responsible for triggering different downstream processes.The Sul1 and Sul2 transceptors are the first identified plasma membrane sensors for extracellular sulfate.High affinity transporters induced upon starvation for their substrate may generally act as transceptors during exit from starvation.

View Article: PubMed Central - PubMed

Affiliation: From the Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, KU Leuven, Kasteelpark Arenberg 31, B-3001 Leuven-Heverlee, Flanders, Belgium, the Department of Molecular Microbiology, VIB, Kasteelpark Arenberg 31, B-3001 Leuven-Heverlee, Flanders, Belgium, and.

Show MeSH
Related in: MedlinePlus