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Expression and preliminary functional profiling of the let-7 family during porcine ovary follicle atresia.

Cao R, Wu WJ, Zhou XL, Xiao P, Wang Y, Liu HL - Mol. Cells (2015)

Bottom Line: In the current study, real-time quantitative polymerase chain reaction showed that the expression levels of let-7 family members in follicles and granulosa cells were similar to our microarray data, in which miRNAs let-7a, let-7b, let-7c, and let-7i were significantly decreased in early atretic and progressively atretic porcine ovary follicles compared with healthy follicles, while let-7g was highly expressed during follicle atresia.In addition, let-7 target genes were predicted and annotated by TargetScan, PicTar, gene ontology and Kyoto encyclopedia of genes and genomes pathways.Our data provide new insight into the association between the let-7 miRNA family in granulosa cell programmed death.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.

ABSTRACT
Most follicles in the mammalian ovary undergo atresia. Granulosa cell apoptosis is a hallmark of follicle atresia. Our previous study using a microRNA (miRNA) microarray showed that the let-7 microRNA family was differentially expressed during follicular atresia. However, whether the let-7 miRNA family members are related to porcine (Sus scrofa) ovary follicular apoptosis is unclear. In the current study, real-time quantitative polymerase chain reaction showed that the expression levels of let-7 family members in follicles and granulosa cells were similar to our microarray data, in which miRNAs let-7a, let-7b, let-7c, and let-7i were significantly decreased in early atretic and progressively atretic porcine ovary follicles compared with healthy follicles, while let-7g was highly expressed during follicle atresia. Furthermore, flow cytometric analysis and Hoechst33342 staining demonstrated that let-7g increased the apoptotic rate of cultured granulosa cells. In addition, let-7 target genes were predicted and annotated by TargetScan, PicTar, gene ontology and Kyoto encyclopedia of genes and genomes pathways. Our data provide new insight into the association between the let-7 miRNA family in granulosa cell programmed death.

No MeSH data available.


Related in: MedlinePlus

Analysis of predicted let-7 target genes by number, function and signaling pathway. (A) Venn diagram analysis of the relationship between predicted let-7a/b/c/i target genes. (B) Main biological processes of predicted let-7a/b/c/i target genes based on GO analysis. (C) Main pathways involving predicted let-7a/b/c/i target genes based on KEGG pathway analysis. (D) Main biological processes of predicted let-7g target genes. (E) Main pathways involving predicted let-7g target genes. -Log10 values (P values), representing significant data from GO and KEGG pathway analyses, are presented in the form of a histogram (SigmaPlot, Systat Software, USA).
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f7-molce-38-4-304: Analysis of predicted let-7 target genes by number, function and signaling pathway. (A) Venn diagram analysis of the relationship between predicted let-7a/b/c/i target genes. (B) Main biological processes of predicted let-7a/b/c/i target genes based on GO analysis. (C) Main pathways involving predicted let-7a/b/c/i target genes based on KEGG pathway analysis. (D) Main biological processes of predicted let-7g target genes. (E) Main pathways involving predicted let-7g target genes. -Log10 values (P values), representing significant data from GO and KEGG pathway analyses, are presented in the form of a histogram (SigmaPlot, Systat Software, USA).

Mentions: Targets prediction, GO analysis and KEGG pathway analysis indicated that let-7a, let-7b, let-7c and let-7i co-regulated 496 genes (Fig. 7A), while let-7g was predicted to target 501 genes. Furthermore, the most relevant biological process involving the target genes of both let-7a/b/c/i (Fig. 7B) and let-7g (Fig. 7D) was regulation of transcription, followed by positive regulation of signal transduction and posttranscriptional regulation of gene expression. These predicted targets of downregulated let-7a/b/c/i (Fig. 7C) and let-7g (Fig. 7E) during follicular atresia may be involved in MAPK, TGF- beta and p53 signaling pathways, which affect cell proliferation, cell cycle or cell survival during follicle atresia.


Expression and preliminary functional profiling of the let-7 family during porcine ovary follicle atresia.

Cao R, Wu WJ, Zhou XL, Xiao P, Wang Y, Liu HL - Mol. Cells (2015)

Analysis of predicted let-7 target genes by number, function and signaling pathway. (A) Venn diagram analysis of the relationship between predicted let-7a/b/c/i target genes. (B) Main biological processes of predicted let-7a/b/c/i target genes based on GO analysis. (C) Main pathways involving predicted let-7a/b/c/i target genes based on KEGG pathway analysis. (D) Main biological processes of predicted let-7g target genes. (E) Main pathways involving predicted let-7g target genes. -Log10 values (P values), representing significant data from GO and KEGG pathway analyses, are presented in the form of a histogram (SigmaPlot, Systat Software, USA).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400304&req=5

f7-molce-38-4-304: Analysis of predicted let-7 target genes by number, function and signaling pathway. (A) Venn diagram analysis of the relationship between predicted let-7a/b/c/i target genes. (B) Main biological processes of predicted let-7a/b/c/i target genes based on GO analysis. (C) Main pathways involving predicted let-7a/b/c/i target genes based on KEGG pathway analysis. (D) Main biological processes of predicted let-7g target genes. (E) Main pathways involving predicted let-7g target genes. -Log10 values (P values), representing significant data from GO and KEGG pathway analyses, are presented in the form of a histogram (SigmaPlot, Systat Software, USA).
Mentions: Targets prediction, GO analysis and KEGG pathway analysis indicated that let-7a, let-7b, let-7c and let-7i co-regulated 496 genes (Fig. 7A), while let-7g was predicted to target 501 genes. Furthermore, the most relevant biological process involving the target genes of both let-7a/b/c/i (Fig. 7B) and let-7g (Fig. 7D) was regulation of transcription, followed by positive regulation of signal transduction and posttranscriptional regulation of gene expression. These predicted targets of downregulated let-7a/b/c/i (Fig. 7C) and let-7g (Fig. 7E) during follicular atresia may be involved in MAPK, TGF- beta and p53 signaling pathways, which affect cell proliferation, cell cycle or cell survival during follicle atresia.

Bottom Line: In the current study, real-time quantitative polymerase chain reaction showed that the expression levels of let-7 family members in follicles and granulosa cells were similar to our microarray data, in which miRNAs let-7a, let-7b, let-7c, and let-7i were significantly decreased in early atretic and progressively atretic porcine ovary follicles compared with healthy follicles, while let-7g was highly expressed during follicle atresia.In addition, let-7 target genes were predicted and annotated by TargetScan, PicTar, gene ontology and Kyoto encyclopedia of genes and genomes pathways.Our data provide new insight into the association between the let-7 miRNA family in granulosa cell programmed death.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.

ABSTRACT
Most follicles in the mammalian ovary undergo atresia. Granulosa cell apoptosis is a hallmark of follicle atresia. Our previous study using a microRNA (miRNA) microarray showed that the let-7 microRNA family was differentially expressed during follicular atresia. However, whether the let-7 miRNA family members are related to porcine (Sus scrofa) ovary follicular apoptosis is unclear. In the current study, real-time quantitative polymerase chain reaction showed that the expression levels of let-7 family members in follicles and granulosa cells were similar to our microarray data, in which miRNAs let-7a, let-7b, let-7c, and let-7i were significantly decreased in early atretic and progressively atretic porcine ovary follicles compared with healthy follicles, while let-7g was highly expressed during follicle atresia. Furthermore, flow cytometric analysis and Hoechst33342 staining demonstrated that let-7g increased the apoptotic rate of cultured granulosa cells. In addition, let-7 target genes were predicted and annotated by TargetScan, PicTar, gene ontology and Kyoto encyclopedia of genes and genomes pathways. Our data provide new insight into the association between the let-7 miRNA family in granulosa cell programmed death.

No MeSH data available.


Related in: MedlinePlus