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Expression and preliminary functional profiling of the let-7 family during porcine ovary follicle atresia.

Cao R, Wu WJ, Zhou XL, Xiao P, Wang Y, Liu HL - Mol. Cells (2015)

Bottom Line: In the current study, real-time quantitative polymerase chain reaction showed that the expression levels of let-7 family members in follicles and granulosa cells were similar to our microarray data, in which miRNAs let-7a, let-7b, let-7c, and let-7i were significantly decreased in early atretic and progressively atretic porcine ovary follicles compared with healthy follicles, while let-7g was highly expressed during follicle atresia.In addition, let-7 target genes were predicted and annotated by TargetScan, PicTar, gene ontology and Kyoto encyclopedia of genes and genomes pathways.Our data provide new insight into the association between the let-7 miRNA family in granulosa cell programmed death.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.

ABSTRACT
Most follicles in the mammalian ovary undergo atresia. Granulosa cell apoptosis is a hallmark of follicle atresia. Our previous study using a microRNA (miRNA) microarray showed that the let-7 microRNA family was differentially expressed during follicular atresia. However, whether the let-7 miRNA family members are related to porcine (Sus scrofa) ovary follicular apoptosis is unclear. In the current study, real-time quantitative polymerase chain reaction showed that the expression levels of let-7 family members in follicles and granulosa cells were similar to our microarray data, in which miRNAs let-7a, let-7b, let-7c, and let-7i were significantly decreased in early atretic and progressively atretic porcine ovary follicles compared with healthy follicles, while let-7g was highly expressed during follicle atresia. Furthermore, flow cytometric analysis and Hoechst33342 staining demonstrated that let-7g increased the apoptotic rate of cultured granulosa cells. In addition, let-7 target genes were predicted and annotated by TargetScan, PicTar, gene ontology and Kyoto encyclopedia of genes and genomes pathways. Our data provide new insight into the association between the let-7 miRNA family in granulosa cell programmed death.

No MeSH data available.


Related in: MedlinePlus

Let-7 miRNA family expression signals during follicular atresia detected by an miRNA microarray assay. Mature let-7a (A), let-7b (B), let-7c (C), and let-7g (D) microarray signals were determined in healthy (H), early atretic (EA), and progressively atretic (PA) follicles. The expressions of let-7a, let-7b and let-7c were downregulated during follicle atresia, while, let-7g expression was upregulated (relative to H). Data are presented as the mean ± SE. A one-way ANOVA test was used for multiple comparisons. P < 0.05 is shown as *, P < 0.01 is shown as **, and P < 0.001 is shown as ***.
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f2-molce-38-4-304: Let-7 miRNA family expression signals during follicular atresia detected by an miRNA microarray assay. Mature let-7a (A), let-7b (B), let-7c (C), and let-7g (D) microarray signals were determined in healthy (H), early atretic (EA), and progressively atretic (PA) follicles. The expressions of let-7a, let-7b and let-7c were downregulated during follicle atresia, while, let-7g expression was upregulated (relative to H). Data are presented as the mean ± SE. A one-way ANOVA test was used for multiple comparisons. P < 0.05 is shown as *, P < 0.01 is shown as **, and P < 0.001 is shown as ***.

Mentions: According to miRBase 21.0 (http://www.mirbase.org), seven porcine mature let-7 miRNAs (let-7a, let-7c, let-7d, let-7e, let-7f, let-7g, and let-7i) have been published. Interestingly, our results showed that all these mature let-7 miRNAs signals could be detected in our chip data, adding let-7b. MiRNA let-7a, let-7b, let-7c and let-7i were significantly decreased in EA and PA porcine ovary follicles relative to H follicles. Interestingly, let-7g was significantly increased during the process of follicle atresia (Fig. 2).


Expression and preliminary functional profiling of the let-7 family during porcine ovary follicle atresia.

Cao R, Wu WJ, Zhou XL, Xiao P, Wang Y, Liu HL - Mol. Cells (2015)

Let-7 miRNA family expression signals during follicular atresia detected by an miRNA microarray assay. Mature let-7a (A), let-7b (B), let-7c (C), and let-7g (D) microarray signals were determined in healthy (H), early atretic (EA), and progressively atretic (PA) follicles. The expressions of let-7a, let-7b and let-7c were downregulated during follicle atresia, while, let-7g expression was upregulated (relative to H). Data are presented as the mean ± SE. A one-way ANOVA test was used for multiple comparisons. P < 0.05 is shown as *, P < 0.01 is shown as **, and P < 0.001 is shown as ***.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400304&req=5

f2-molce-38-4-304: Let-7 miRNA family expression signals during follicular atresia detected by an miRNA microarray assay. Mature let-7a (A), let-7b (B), let-7c (C), and let-7g (D) microarray signals were determined in healthy (H), early atretic (EA), and progressively atretic (PA) follicles. The expressions of let-7a, let-7b and let-7c were downregulated during follicle atresia, while, let-7g expression was upregulated (relative to H). Data are presented as the mean ± SE. A one-way ANOVA test was used for multiple comparisons. P < 0.05 is shown as *, P < 0.01 is shown as **, and P < 0.001 is shown as ***.
Mentions: According to miRBase 21.0 (http://www.mirbase.org), seven porcine mature let-7 miRNAs (let-7a, let-7c, let-7d, let-7e, let-7f, let-7g, and let-7i) have been published. Interestingly, our results showed that all these mature let-7 miRNAs signals could be detected in our chip data, adding let-7b. MiRNA let-7a, let-7b, let-7c and let-7i were significantly decreased in EA and PA porcine ovary follicles relative to H follicles. Interestingly, let-7g was significantly increased during the process of follicle atresia (Fig. 2).

Bottom Line: In the current study, real-time quantitative polymerase chain reaction showed that the expression levels of let-7 family members in follicles and granulosa cells were similar to our microarray data, in which miRNAs let-7a, let-7b, let-7c, and let-7i were significantly decreased in early atretic and progressively atretic porcine ovary follicles compared with healthy follicles, while let-7g was highly expressed during follicle atresia.In addition, let-7 target genes were predicted and annotated by TargetScan, PicTar, gene ontology and Kyoto encyclopedia of genes and genomes pathways.Our data provide new insight into the association between the let-7 miRNA family in granulosa cell programmed death.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.

ABSTRACT
Most follicles in the mammalian ovary undergo atresia. Granulosa cell apoptosis is a hallmark of follicle atresia. Our previous study using a microRNA (miRNA) microarray showed that the let-7 microRNA family was differentially expressed during follicular atresia. However, whether the let-7 miRNA family members are related to porcine (Sus scrofa) ovary follicular apoptosis is unclear. In the current study, real-time quantitative polymerase chain reaction showed that the expression levels of let-7 family members in follicles and granulosa cells were similar to our microarray data, in which miRNAs let-7a, let-7b, let-7c, and let-7i were significantly decreased in early atretic and progressively atretic porcine ovary follicles compared with healthy follicles, while let-7g was highly expressed during follicle atresia. Furthermore, flow cytometric analysis and Hoechst33342 staining demonstrated that let-7g increased the apoptotic rate of cultured granulosa cells. In addition, let-7 target genes were predicted and annotated by TargetScan, PicTar, gene ontology and Kyoto encyclopedia of genes and genomes pathways. Our data provide new insight into the association between the let-7 miRNA family in granulosa cell programmed death.

No MeSH data available.


Related in: MedlinePlus