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Expression and preliminary functional profiling of the let-7 family during porcine ovary follicle atresia.

Cao R, Wu WJ, Zhou XL, Xiao P, Wang Y, Liu HL - Mol. Cells (2015)

Bottom Line: In the current study, real-time quantitative polymerase chain reaction showed that the expression levels of let-7 family members in follicles and granulosa cells were similar to our microarray data, in which miRNAs let-7a, let-7b, let-7c, and let-7i were significantly decreased in early atretic and progressively atretic porcine ovary follicles compared with healthy follicles, while let-7g was highly expressed during follicle atresia.In addition, let-7 target genes were predicted and annotated by TargetScan, PicTar, gene ontology and Kyoto encyclopedia of genes and genomes pathways.Our data provide new insight into the association between the let-7 miRNA family in granulosa cell programmed death.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.

ABSTRACT
Most follicles in the mammalian ovary undergo atresia. Granulosa cell apoptosis is a hallmark of follicle atresia. Our previous study using a microRNA (miRNA) microarray showed that the let-7 microRNA family was differentially expressed during follicular atresia. However, whether the let-7 miRNA family members are related to porcine (Sus scrofa) ovary follicular apoptosis is unclear. In the current study, real-time quantitative polymerase chain reaction showed that the expression levels of let-7 family members in follicles and granulosa cells were similar to our microarray data, in which miRNAs let-7a, let-7b, let-7c, and let-7i were significantly decreased in early atretic and progressively atretic porcine ovary follicles compared with healthy follicles, while let-7g was highly expressed during follicle atresia. Furthermore, flow cytometric analysis and Hoechst33342 staining demonstrated that let-7g increased the apoptotic rate of cultured granulosa cells. In addition, let-7 target genes were predicted and annotated by TargetScan, PicTar, gene ontology and Kyoto encyclopedia of genes and genomes pathways. Our data provide new insight into the association between the let-7 miRNA family in granulosa cell programmed death.

No MeSH data available.


Related in: MedlinePlus

Phenotypic characteristic of healthy, early atretic and progressively atretic porcine ovary follicles. H, Healthy follicles. Healthy follicles had visible cumulus-oocyte complex (COC) and clear follicular fluid. EA, Early atretic follicles. Early atretic follicles had gradually separated COC and slightly turbid follicular fluid. PA, Progressively atretic follicles. Progressively atretic follicles had turbid follicular fluid and were barely vascularized.
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f1-molce-38-4-304: Phenotypic characteristic of healthy, early atretic and progressively atretic porcine ovary follicles. H, Healthy follicles. Healthy follicles had visible cumulus-oocyte complex (COC) and clear follicular fluid. EA, Early atretic follicles. Early atretic follicles had gradually separated COC and slightly turbid follicular fluid. PA, Progressively atretic follicles. Progressively atretic follicles had turbid follicular fluid and were barely vascularized.

Mentions: On the basis of morphology, visible COCs (Bortul et al., 2003), clear follicular fluid, and well vascularized characterized H follicles. COCs and granulosa cells layer were somewhat separated and slightly turbid follicular fluid could be observed in EA follicles. PA follicles had turbid follicular fluid and were barely vascularized (Carson et al., 1979; Hay et al., 1976). The phenotype of H, EA and PA follicles is shown in Fig. 1.


Expression and preliminary functional profiling of the let-7 family during porcine ovary follicle atresia.

Cao R, Wu WJ, Zhou XL, Xiao P, Wang Y, Liu HL - Mol. Cells (2015)

Phenotypic characteristic of healthy, early atretic and progressively atretic porcine ovary follicles. H, Healthy follicles. Healthy follicles had visible cumulus-oocyte complex (COC) and clear follicular fluid. EA, Early atretic follicles. Early atretic follicles had gradually separated COC and slightly turbid follicular fluid. PA, Progressively atretic follicles. Progressively atretic follicles had turbid follicular fluid and were barely vascularized.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400304&req=5

f1-molce-38-4-304: Phenotypic characteristic of healthy, early atretic and progressively atretic porcine ovary follicles. H, Healthy follicles. Healthy follicles had visible cumulus-oocyte complex (COC) and clear follicular fluid. EA, Early atretic follicles. Early atretic follicles had gradually separated COC and slightly turbid follicular fluid. PA, Progressively atretic follicles. Progressively atretic follicles had turbid follicular fluid and were barely vascularized.
Mentions: On the basis of morphology, visible COCs (Bortul et al., 2003), clear follicular fluid, and well vascularized characterized H follicles. COCs and granulosa cells layer were somewhat separated and slightly turbid follicular fluid could be observed in EA follicles. PA follicles had turbid follicular fluid and were barely vascularized (Carson et al., 1979; Hay et al., 1976). The phenotype of H, EA and PA follicles is shown in Fig. 1.

Bottom Line: In the current study, real-time quantitative polymerase chain reaction showed that the expression levels of let-7 family members in follicles and granulosa cells were similar to our microarray data, in which miRNAs let-7a, let-7b, let-7c, and let-7i were significantly decreased in early atretic and progressively atretic porcine ovary follicles compared with healthy follicles, while let-7g was highly expressed during follicle atresia.In addition, let-7 target genes were predicted and annotated by TargetScan, PicTar, gene ontology and Kyoto encyclopedia of genes and genomes pathways.Our data provide new insight into the association between the let-7 miRNA family in granulosa cell programmed death.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.

ABSTRACT
Most follicles in the mammalian ovary undergo atresia. Granulosa cell apoptosis is a hallmark of follicle atresia. Our previous study using a microRNA (miRNA) microarray showed that the let-7 microRNA family was differentially expressed during follicular atresia. However, whether the let-7 miRNA family members are related to porcine (Sus scrofa) ovary follicular apoptosis is unclear. In the current study, real-time quantitative polymerase chain reaction showed that the expression levels of let-7 family members in follicles and granulosa cells were similar to our microarray data, in which miRNAs let-7a, let-7b, let-7c, and let-7i were significantly decreased in early atretic and progressively atretic porcine ovary follicles compared with healthy follicles, while let-7g was highly expressed during follicle atresia. Furthermore, flow cytometric analysis and Hoechst33342 staining demonstrated that let-7g increased the apoptotic rate of cultured granulosa cells. In addition, let-7 target genes were predicted and annotated by TargetScan, PicTar, gene ontology and Kyoto encyclopedia of genes and genomes pathways. Our data provide new insight into the association between the let-7 miRNA family in granulosa cell programmed death.

No MeSH data available.


Related in: MedlinePlus