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Detection of a 640-bp deletion in the Aggregatibacter actinomycetemcomitans leukotoxin promoter region in isolates from an adolescent of Ethiopian origin.

Claesson R, Gudmundson J, Åberg CH, Haubek D, Johansson A - J Oral Microbiol (2015)

Bottom Line: The expression of the leukotoxin of Aggregatibacter actinomycetemcomitans is regulated by the leukotoxin promoter.A 530-bp deletion or an 886-bp insertion sequence (IS) element in this region has earlier been described in highly leukotoxic isolates.Here, we report on highly leukotoxic isolate with a 640-bp deletion, which was detected in an adolescent of Ethiopian origin.

View Article: PubMed Central - PubMed

Affiliation: Division of Oral Microbiology, Department of Odontology, Umeå University, Umeå, Sweden; rolf.claesson@odont.umu.se.

ABSTRACT
The expression of the leukotoxin of Aggregatibacter actinomycetemcomitans is regulated by the leukotoxin promoter. A 530-bp deletion or an 886-bp insertion sequence (IS) element in this region has earlier been described in highly leukotoxic isolates. Here, we report on highly leukotoxic isolate with a 640-bp deletion, which was detected in an adolescent of Ethiopian origin.

No MeSH data available.


AP-PCR banding pattern of HK 1651 (JP2) [1] and isolate 456A1 [2] obtained by use of primers OPB-3, OPB-7, and OPB-13. Molecular weight marker (M).
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Figure 0004: AP-PCR banding pattern of HK 1651 (JP2) [1] and isolate 456A1 [2] obtained by use of primers OPB-3, OPB-7, and OPB-13. Molecular weight marker (M).

Mentions: Genetic similarities between the 456A1 isolate and a JP2 genotype strain were indicated by AP-PCR (arbitrarily primed PCR) analyzes (6) (Fig. 4). This AP-PCR pattern has been detected in other JP2 genotype isolates, as well as among some of the serotype b non-JP2 genotype isolates (6).


Detection of a 640-bp deletion in the Aggregatibacter actinomycetemcomitans leukotoxin promoter region in isolates from an adolescent of Ethiopian origin.

Claesson R, Gudmundson J, Åberg CH, Haubek D, Johansson A - J Oral Microbiol (2015)

AP-PCR banding pattern of HK 1651 (JP2) [1] and isolate 456A1 [2] obtained by use of primers OPB-3, OPB-7, and OPB-13. Molecular weight marker (M).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400299&req=5

Figure 0004: AP-PCR banding pattern of HK 1651 (JP2) [1] and isolate 456A1 [2] obtained by use of primers OPB-3, OPB-7, and OPB-13. Molecular weight marker (M).
Mentions: Genetic similarities between the 456A1 isolate and a JP2 genotype strain were indicated by AP-PCR (arbitrarily primed PCR) analyzes (6) (Fig. 4). This AP-PCR pattern has been detected in other JP2 genotype isolates, as well as among some of the serotype b non-JP2 genotype isolates (6).

Bottom Line: The expression of the leukotoxin of Aggregatibacter actinomycetemcomitans is regulated by the leukotoxin promoter.A 530-bp deletion or an 886-bp insertion sequence (IS) element in this region has earlier been described in highly leukotoxic isolates.Here, we report on highly leukotoxic isolate with a 640-bp deletion, which was detected in an adolescent of Ethiopian origin.

View Article: PubMed Central - PubMed

Affiliation: Division of Oral Microbiology, Department of Odontology, Umeå University, Umeå, Sweden; rolf.claesson@odont.umu.se.

ABSTRACT
The expression of the leukotoxin of Aggregatibacter actinomycetemcomitans is regulated by the leukotoxin promoter. A 530-bp deletion or an 886-bp insertion sequence (IS) element in this region has earlier been described in highly leukotoxic isolates. Here, we report on highly leukotoxic isolate with a 640-bp deletion, which was detected in an adolescent of Ethiopian origin.

No MeSH data available.