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Detection of a 640-bp deletion in the Aggregatibacter actinomycetemcomitans leukotoxin promoter region in isolates from an adolescent of Ethiopian origin.

Claesson R, Gudmundson J, Åberg CH, Haubek D, Johansson A - J Oral Microbiol (2015)

Bottom Line: The expression of the leukotoxin of Aggregatibacter actinomycetemcomitans is regulated by the leukotoxin promoter.A 530-bp deletion or an 886-bp insertion sequence (IS) element in this region has earlier been described in highly leukotoxic isolates.Here, we report on highly leukotoxic isolate with a 640-bp deletion, which was detected in an adolescent of Ethiopian origin.

View Article: PubMed Central - PubMed

Affiliation: Division of Oral Microbiology, Department of Odontology, Umeå University, Umeå, Sweden; rolf.claesson@odont.umu.se.

ABSTRACT
The expression of the leukotoxin of Aggregatibacter actinomycetemcomitans is regulated by the leukotoxin promoter. A 530-bp deletion or an 886-bp insertion sequence (IS) element in this region has earlier been described in highly leukotoxic isolates. Here, we report on highly leukotoxic isolate with a 640-bp deletion, which was detected in an adolescent of Ethiopian origin.

No MeSH data available.


Related in: MedlinePlus

A. actinomycetemcomitans isolates 456A1 [1], 456A2 [2], sample 455 [3], sample 456 [4], JP2 [5], and D7s [6]. (a) Strain with a complete leukotoxin promoter region, (b) strain with a 530-bp deletion in the leukotoxin promoter region, (c) strain with a 640-bp deletion in the leukotoxin promoter region. Molecular weight marker (M).
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Figure 0002: A. actinomycetemcomitans isolates 456A1 [1], 456A2 [2], sample 455 [3], sample 456 [4], JP2 [5], and D7s [6]. (a) Strain with a complete leukotoxin promoter region, (b) strain with a 530-bp deletion in the leukotoxin promoter region, (c) strain with a 640-bp deletion in the leukotoxin promoter region. Molecular weight marker (M).

Mentions: Recently, we identified a leukotoxin promoter type of A. actinomycetemcomitans, which has not previously been reported. It was detected in two periodontal plaque samples collected from a 16-yr old female periodontitis patient of Ethiopian descent (Fig. 2). The samples were sent in vials containing VMGA III medium (20) to our clinical laboratory at the Dental School in Umeå, Sweden. After a procedure as described (17), it was found that the plaque samples contained high proportions of A. actinomycetemcomitans (Table 1). According to the routine procedure, two isolates (456A1, 456A2) were serotyped according to Suzuki and co-workers (21). Since the two isolates belonged to serotype b, they were typed according to the leukotoxin promoter as described (22). Surprisingly, both isolates were characterized by a leukotoxin promoter region that was approximately 100 bp smaller than the JP2 promoter region, i.e., they harboured a previously undescribed promoter type (Figs. 1 and 2). This finding was confirmed when the bacterial DNA, taken directly from the samples (455V and 456V) in the transport vials (VMGA), was amplified with leukotoxin promoter-specific primers (Fig. 2). The identical characteristics of the analyzed isolates and samples indicate that the patient most likely is colonized by a single clone of A. actinomycetemcomitans.


Detection of a 640-bp deletion in the Aggregatibacter actinomycetemcomitans leukotoxin promoter region in isolates from an adolescent of Ethiopian origin.

Claesson R, Gudmundson J, Åberg CH, Haubek D, Johansson A - J Oral Microbiol (2015)

A. actinomycetemcomitans isolates 456A1 [1], 456A2 [2], sample 455 [3], sample 456 [4], JP2 [5], and D7s [6]. (a) Strain with a complete leukotoxin promoter region, (b) strain with a 530-bp deletion in the leukotoxin promoter region, (c) strain with a 640-bp deletion in the leukotoxin promoter region. Molecular weight marker (M).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400299&req=5

Figure 0002: A. actinomycetemcomitans isolates 456A1 [1], 456A2 [2], sample 455 [3], sample 456 [4], JP2 [5], and D7s [6]. (a) Strain with a complete leukotoxin promoter region, (b) strain with a 530-bp deletion in the leukotoxin promoter region, (c) strain with a 640-bp deletion in the leukotoxin promoter region. Molecular weight marker (M).
Mentions: Recently, we identified a leukotoxin promoter type of A. actinomycetemcomitans, which has not previously been reported. It was detected in two periodontal plaque samples collected from a 16-yr old female periodontitis patient of Ethiopian descent (Fig. 2). The samples were sent in vials containing VMGA III medium (20) to our clinical laboratory at the Dental School in Umeå, Sweden. After a procedure as described (17), it was found that the plaque samples contained high proportions of A. actinomycetemcomitans (Table 1). According to the routine procedure, two isolates (456A1, 456A2) were serotyped according to Suzuki and co-workers (21). Since the two isolates belonged to serotype b, they were typed according to the leukotoxin promoter as described (22). Surprisingly, both isolates were characterized by a leukotoxin promoter region that was approximately 100 bp smaller than the JP2 promoter region, i.e., they harboured a previously undescribed promoter type (Figs. 1 and 2). This finding was confirmed when the bacterial DNA, taken directly from the samples (455V and 456V) in the transport vials (VMGA), was amplified with leukotoxin promoter-specific primers (Fig. 2). The identical characteristics of the analyzed isolates and samples indicate that the patient most likely is colonized by a single clone of A. actinomycetemcomitans.

Bottom Line: The expression of the leukotoxin of Aggregatibacter actinomycetemcomitans is regulated by the leukotoxin promoter.A 530-bp deletion or an 886-bp insertion sequence (IS) element in this region has earlier been described in highly leukotoxic isolates.Here, we report on highly leukotoxic isolate with a 640-bp deletion, which was detected in an adolescent of Ethiopian origin.

View Article: PubMed Central - PubMed

Affiliation: Division of Oral Microbiology, Department of Odontology, Umeå University, Umeå, Sweden; rolf.claesson@odont.umu.se.

ABSTRACT
The expression of the leukotoxin of Aggregatibacter actinomycetemcomitans is regulated by the leukotoxin promoter. A 530-bp deletion or an 886-bp insertion sequence (IS) element in this region has earlier been described in highly leukotoxic isolates. Here, we report on highly leukotoxic isolate with a 640-bp deletion, which was detected in an adolescent of Ethiopian origin.

No MeSH data available.


Related in: MedlinePlus