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Detection of a 640-bp deletion in the Aggregatibacter actinomycetemcomitans leukotoxin promoter region in isolates from an adolescent of Ethiopian origin.

Claesson R, Gudmundson J, Åberg CH, Haubek D, Johansson A - J Oral Microbiol (2015)

Bottom Line: The expression of the leukotoxin of Aggregatibacter actinomycetemcomitans is regulated by the leukotoxin promoter.A 530-bp deletion or an 886-bp insertion sequence (IS) element in this region has earlier been described in highly leukotoxic isolates.Here, we report on highly leukotoxic isolate with a 640-bp deletion, which was detected in an adolescent of Ethiopian origin.

View Article: PubMed Central - PubMed

Affiliation: Division of Oral Microbiology, Department of Odontology, Umeå University, Umeå, Sweden; rolf.claesson@odont.umu.se.

ABSTRACT
The expression of the leukotoxin of Aggregatibacter actinomycetemcomitans is regulated by the leukotoxin promoter. A 530-bp deletion or an 886-bp insertion sequence (IS) element in this region has earlier been described in highly leukotoxic isolates. Here, we report on highly leukotoxic isolate with a 640-bp deletion, which was detected in an adolescent of Ethiopian origin.

No MeSH data available.


Schematic illustration of the leukotoxin promoter from A. actinomycetemcomitans genotype JP2, non-JP2, IS, and the most recent isolate 456A1. (a) The leukotoxin promoter region of A. actinomycetemcomitans (non-JP2): 1,109 bp (the sequence from the first nucleotide after the stop codon of glyA to the stop codon of promoter region is incorporated) (11). (b) The position of the 530-bp deletion (JP2) within the promoter region (9). (c) The position of the 886-bp insertion sequence (IS) within the promoter region (10). (d) The position of the 640-bp deletion (456 A1 isolate) within the promoter region (this study).
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Figure 0001: Schematic illustration of the leukotoxin promoter from A. actinomycetemcomitans genotype JP2, non-JP2, IS, and the most recent isolate 456A1. (a) The leukotoxin promoter region of A. actinomycetemcomitans (non-JP2): 1,109 bp (the sequence from the first nucleotide after the stop codon of glyA to the stop codon of promoter region is incorporated) (11). (b) The position of the 530-bp deletion (JP2) within the promoter region (9). (c) The position of the 886-bp insertion sequence (IS) within the promoter region (10). (d) The position of the 640-bp deletion (456 A1 isolate) within the promoter region (this study).

Mentions: The Gram-negative oral bacterial species, Aggregatibacter actinomycetemcomitans, expresses a leukotoxin that links the bacterium to aggressive periodontitis (1–6). The toxin expression, which is associated with four structural genes (ltxA, ltxB, ltxC, and ltxD), is regulated by the leukotoxin promoter (Fig. 1). Although the genes of the leukotoxin operon appear to be present in all strains of A. actinomycetemcomitans, the toxin expression varies substantially between different strains (7–9). High expression of leukotoxin has been detected in A. actinomycetemcomitans isolates with a 530-bp deletion in the promoter region and in isolates carrying an 886-bp IS element in the same region (7–10) (Fig. 1).


Detection of a 640-bp deletion in the Aggregatibacter actinomycetemcomitans leukotoxin promoter region in isolates from an adolescent of Ethiopian origin.

Claesson R, Gudmundson J, Åberg CH, Haubek D, Johansson A - J Oral Microbiol (2015)

Schematic illustration of the leukotoxin promoter from A. actinomycetemcomitans genotype JP2, non-JP2, IS, and the most recent isolate 456A1. (a) The leukotoxin promoter region of A. actinomycetemcomitans (non-JP2): 1,109 bp (the sequence from the first nucleotide after the stop codon of glyA to the stop codon of promoter region is incorporated) (11). (b) The position of the 530-bp deletion (JP2) within the promoter region (9). (c) The position of the 886-bp insertion sequence (IS) within the promoter region (10). (d) The position of the 640-bp deletion (456 A1 isolate) within the promoter region (this study).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400299&req=5

Figure 0001: Schematic illustration of the leukotoxin promoter from A. actinomycetemcomitans genotype JP2, non-JP2, IS, and the most recent isolate 456A1. (a) The leukotoxin promoter region of A. actinomycetemcomitans (non-JP2): 1,109 bp (the sequence from the first nucleotide after the stop codon of glyA to the stop codon of promoter region is incorporated) (11). (b) The position of the 530-bp deletion (JP2) within the promoter region (9). (c) The position of the 886-bp insertion sequence (IS) within the promoter region (10). (d) The position of the 640-bp deletion (456 A1 isolate) within the promoter region (this study).
Mentions: The Gram-negative oral bacterial species, Aggregatibacter actinomycetemcomitans, expresses a leukotoxin that links the bacterium to aggressive periodontitis (1–6). The toxin expression, which is associated with four structural genes (ltxA, ltxB, ltxC, and ltxD), is regulated by the leukotoxin promoter (Fig. 1). Although the genes of the leukotoxin operon appear to be present in all strains of A. actinomycetemcomitans, the toxin expression varies substantially between different strains (7–9). High expression of leukotoxin has been detected in A. actinomycetemcomitans isolates with a 530-bp deletion in the promoter region and in isolates carrying an 886-bp IS element in the same region (7–10) (Fig. 1).

Bottom Line: The expression of the leukotoxin of Aggregatibacter actinomycetemcomitans is regulated by the leukotoxin promoter.A 530-bp deletion or an 886-bp insertion sequence (IS) element in this region has earlier been described in highly leukotoxic isolates.Here, we report on highly leukotoxic isolate with a 640-bp deletion, which was detected in an adolescent of Ethiopian origin.

View Article: PubMed Central - PubMed

Affiliation: Division of Oral Microbiology, Department of Odontology, Umeå University, Umeå, Sweden; rolf.claesson@odont.umu.se.

ABSTRACT
The expression of the leukotoxin of Aggregatibacter actinomycetemcomitans is regulated by the leukotoxin promoter. A 530-bp deletion or an 886-bp insertion sequence (IS) element in this region has earlier been described in highly leukotoxic isolates. Here, we report on highly leukotoxic isolate with a 640-bp deletion, which was detected in an adolescent of Ethiopian origin.

No MeSH data available.