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In vivo assessment of cardiac metabolism and function in the abdominal aortic banding model of compensated cardiac hypertrophy.

Seymour AM, Giles L, Ball V, Miller JJ, Clarke K, Carr CA, Tyler DJ - Cardiovasc. Res. (2015)

Bottom Line: Pyruvate dehydrogenase flux was unchanged in the hypertrophied animals at any time point, but increased incorporation of the (13)C label into lactate was observed by 9 weeks and maintained at 14 weeks, indicative of enhanced glycolysis.Hypertrophied hearts revealed little evidence of a switch towards increased glucose oxidation but rather an uncoupling of glycolytic metabolism from glucose oxidation.This was maintained under conditions of dietary stress provided by a WD but, at this compensated phase of hypertrophy, did not result in any contractile dysfunction.

View Article: PubMed Central - PubMed

Affiliation: School of Biological, Biomedical and Environmental Sciences, University of Hull, Hull HU6 7RX, UK.

No MeSH data available.


Related in: MedlinePlus

(A) Cardiac PDK4 protein expression and (B) cardiac TAG content at 14 weeks post-surgical induction of cardiac hypertrophy (AAB) and exposure to standard chow or WD. *P < 0.05 WD groups compared with standard chow groups. Group sizes as indicated on individual bars. X represents an internal standard on the western blot.
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Figure 8: (A) Cardiac PDK4 protein expression and (B) cardiac TAG content at 14 weeks post-surgical induction of cardiac hypertrophy (AAB) and exposure to standard chow or WD. *P < 0.05 WD groups compared with standard chow groups. Group sizes as indicated on individual bars. X represents an internal standard on the western blot.

Mentions: In vitro analysis of the expression of the key inhibitory enzyme PDK4 at the 14-week time point reflected the in vivo hyperpolarized 13C MR findings with no alteration in expression in AAB or control groups within the same dietary group but a marked elevation of PDK4 expression in WD groups (Figure 8A). In vitro analysis of cardiac TAG levels demonstrated that they were unaffected by either AAB or the WD (Figure 8B), indicating no elevated deposition of lipids in the cardiac tissue.


In vivo assessment of cardiac metabolism and function in the abdominal aortic banding model of compensated cardiac hypertrophy.

Seymour AM, Giles L, Ball V, Miller JJ, Clarke K, Carr CA, Tyler DJ - Cardiovasc. Res. (2015)

(A) Cardiac PDK4 protein expression and (B) cardiac TAG content at 14 weeks post-surgical induction of cardiac hypertrophy (AAB) and exposure to standard chow or WD. *P < 0.05 WD groups compared with standard chow groups. Group sizes as indicated on individual bars. X represents an internal standard on the western blot.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400188&req=5

Figure 8: (A) Cardiac PDK4 protein expression and (B) cardiac TAG content at 14 weeks post-surgical induction of cardiac hypertrophy (AAB) and exposure to standard chow or WD. *P < 0.05 WD groups compared with standard chow groups. Group sizes as indicated on individual bars. X represents an internal standard on the western blot.
Mentions: In vitro analysis of the expression of the key inhibitory enzyme PDK4 at the 14-week time point reflected the in vivo hyperpolarized 13C MR findings with no alteration in expression in AAB or control groups within the same dietary group but a marked elevation of PDK4 expression in WD groups (Figure 8A). In vitro analysis of cardiac TAG levels demonstrated that they were unaffected by either AAB or the WD (Figure 8B), indicating no elevated deposition of lipids in the cardiac tissue.

Bottom Line: Pyruvate dehydrogenase flux was unchanged in the hypertrophied animals at any time point, but increased incorporation of the (13)C label into lactate was observed by 9 weeks and maintained at 14 weeks, indicative of enhanced glycolysis.Hypertrophied hearts revealed little evidence of a switch towards increased glucose oxidation but rather an uncoupling of glycolytic metabolism from glucose oxidation.This was maintained under conditions of dietary stress provided by a WD but, at this compensated phase of hypertrophy, did not result in any contractile dysfunction.

View Article: PubMed Central - PubMed

Affiliation: School of Biological, Biomedical and Environmental Sciences, University of Hull, Hull HU6 7RX, UK.

No MeSH data available.


Related in: MedlinePlus