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In vivo assessment of cardiac metabolism and function in the abdominal aortic banding model of compensated cardiac hypertrophy.

Seymour AM, Giles L, Ball V, Miller JJ, Clarke K, Carr CA, Tyler DJ - Cardiovasc. Res. (2015)

Bottom Line: Pyruvate dehydrogenase flux was unchanged in the hypertrophied animals at any time point, but increased incorporation of the (13)C label into lactate was observed by 9 weeks and maintained at 14 weeks, indicative of enhanced glycolysis.Hypertrophied hearts revealed little evidence of a switch towards increased glucose oxidation but rather an uncoupling of glycolytic metabolism from glucose oxidation.This was maintained under conditions of dietary stress provided by a WD but, at this compensated phase of hypertrophy, did not result in any contractile dysfunction.

View Article: PubMed Central - PubMed

Affiliation: School of Biological, Biomedical and Environmental Sciences, University of Hull, Hull HU6 7RX, UK.

No MeSH data available.


Related in: MedlinePlus

Ratio of (A) citrate, (B) glutamate, and (C) acetylcarnitine to injected hyperpolarized [2-13C]pyruvate at 4, 9, and 14 weeks post-surgical induction of cardiac hypertrophy and exposure to standard chow or WD.$P < 0.05 WDA AB group compared with chow AAB group and *P < 0.05 WD groups compared with standard chow groups. Group sizes as indicated on individual bars.
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Figure 7: Ratio of (A) citrate, (B) glutamate, and (C) acetylcarnitine to injected hyperpolarized [2-13C]pyruvate at 4, 9, and 14 weeks post-surgical induction of cardiac hypertrophy and exposure to standard chow or WD.$P < 0.05 WDA AB group compared with chow AAB group and *P < 0.05 WD groups compared with standard chow groups. Group sizes as indicated on individual bars.

Mentions: Hyperpolarized [2-13C]pyruvate was used to determine incorporation of pyruvate into TCA cycle intermediates. Within the AAB groups, there was no change in the [13C]metabolite:[2-13C]pyruvate ratio for either citrate or glutamate, independent of diet (Figure 7A and B). Given that PDH flux was unaltered in the hypertrophied hearts, these observations would indicate that there was no increase in the flux of glucose into the TCA cycle during this phase of hypertrophic development. These findings are thus suggestive of a compensatory phase of cardiac hypertrophy with no adverse impact from the high-fat, high-sucrose content of the WD.


In vivo assessment of cardiac metabolism and function in the abdominal aortic banding model of compensated cardiac hypertrophy.

Seymour AM, Giles L, Ball V, Miller JJ, Clarke K, Carr CA, Tyler DJ - Cardiovasc. Res. (2015)

Ratio of (A) citrate, (B) glutamate, and (C) acetylcarnitine to injected hyperpolarized [2-13C]pyruvate at 4, 9, and 14 weeks post-surgical induction of cardiac hypertrophy and exposure to standard chow or WD.$P < 0.05 WDA AB group compared with chow AAB group and *P < 0.05 WD groups compared with standard chow groups. Group sizes as indicated on individual bars.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400188&req=5

Figure 7: Ratio of (A) citrate, (B) glutamate, and (C) acetylcarnitine to injected hyperpolarized [2-13C]pyruvate at 4, 9, and 14 weeks post-surgical induction of cardiac hypertrophy and exposure to standard chow or WD.$P < 0.05 WDA AB group compared with chow AAB group and *P < 0.05 WD groups compared with standard chow groups. Group sizes as indicated on individual bars.
Mentions: Hyperpolarized [2-13C]pyruvate was used to determine incorporation of pyruvate into TCA cycle intermediates. Within the AAB groups, there was no change in the [13C]metabolite:[2-13C]pyruvate ratio for either citrate or glutamate, independent of diet (Figure 7A and B). Given that PDH flux was unaltered in the hypertrophied hearts, these observations would indicate that there was no increase in the flux of glucose into the TCA cycle during this phase of hypertrophic development. These findings are thus suggestive of a compensatory phase of cardiac hypertrophy with no adverse impact from the high-fat, high-sucrose content of the WD.

Bottom Line: Pyruvate dehydrogenase flux was unchanged in the hypertrophied animals at any time point, but increased incorporation of the (13)C label into lactate was observed by 9 weeks and maintained at 14 weeks, indicative of enhanced glycolysis.Hypertrophied hearts revealed little evidence of a switch towards increased glucose oxidation but rather an uncoupling of glycolytic metabolism from glucose oxidation.This was maintained under conditions of dietary stress provided by a WD but, at this compensated phase of hypertrophy, did not result in any contractile dysfunction.

View Article: PubMed Central - PubMed

Affiliation: School of Biological, Biomedical and Environmental Sciences, University of Hull, Hull HU6 7RX, UK.

No MeSH data available.


Related in: MedlinePlus