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In vivo assessment of cardiac metabolism and function in the abdominal aortic banding model of compensated cardiac hypertrophy.

Seymour AM, Giles L, Ball V, Miller JJ, Clarke K, Carr CA, Tyler DJ - Cardiovasc. Res. (2015)

Bottom Line: Pyruvate dehydrogenase flux was unchanged in the hypertrophied animals at any time point, but increased incorporation of the (13)C label into lactate was observed by 9 weeks and maintained at 14 weeks, indicative of enhanced glycolysis.Hypertrophied hearts revealed little evidence of a switch towards increased glucose oxidation but rather an uncoupling of glycolytic metabolism from glucose oxidation.This was maintained under conditions of dietary stress provided by a WD but, at this compensated phase of hypertrophy, did not result in any contractile dysfunction.

View Article: PubMed Central - PubMed

Affiliation: School of Biological, Biomedical and Environmental Sciences, University of Hull, Hull HU6 7RX, UK.

No MeSH data available.


Related in: MedlinePlus

In vivo rates of incorporation of hyperpolarized [1-13C]pyruvate into (A) bicarbonate (pyruvate dehydrogenase flux) and (B) lactate at 4, 9, and 14 weeks post-surgical induction of cardiac hypertrophy and exposure to standard chow or WD. *P < 0.05 in WD groups vs. standard chow and §P < 0.05 in AAB groups vs. respective sham controls. Group sizes as indicated on individual bars.
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Figure 6: In vivo rates of incorporation of hyperpolarized [1-13C]pyruvate into (A) bicarbonate (pyruvate dehydrogenase flux) and (B) lactate at 4, 9, and 14 weeks post-surgical induction of cardiac hypertrophy and exposure to standard chow or WD. *P < 0.05 in WD groups vs. standard chow and §P < 0.05 in AAB groups vs. respective sham controls. Group sizes as indicated on individual bars.

Mentions: PDH flux was determined in vivo through incorporation of the hyperpolarized 13C label from [1-13C]pyruvate into bicarbonate as previously demonstrated21 (Figure 5). Rates of 13C label incorporation into bicarbonate in AAB groups at the different time points were not significantly different from their respective controls (Figure 6A), implying little effect of AAB on PDH flux in vivo and thus glucose oxidation over the time course of hypertrophic development. At all time points however, dietary manipulation resulted in a marked reduction in 13C label incorporation into bicarbonate, indicating that PDH flux was reduced in the WD groups compared with their respective standard chow groups as would be predicted from the Randle cycle.26


In vivo assessment of cardiac metabolism and function in the abdominal aortic banding model of compensated cardiac hypertrophy.

Seymour AM, Giles L, Ball V, Miller JJ, Clarke K, Carr CA, Tyler DJ - Cardiovasc. Res. (2015)

In vivo rates of incorporation of hyperpolarized [1-13C]pyruvate into (A) bicarbonate (pyruvate dehydrogenase flux) and (B) lactate at 4, 9, and 14 weeks post-surgical induction of cardiac hypertrophy and exposure to standard chow or WD. *P < 0.05 in WD groups vs. standard chow and §P < 0.05 in AAB groups vs. respective sham controls. Group sizes as indicated on individual bars.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400188&req=5

Figure 6: In vivo rates of incorporation of hyperpolarized [1-13C]pyruvate into (A) bicarbonate (pyruvate dehydrogenase flux) and (B) lactate at 4, 9, and 14 weeks post-surgical induction of cardiac hypertrophy and exposure to standard chow or WD. *P < 0.05 in WD groups vs. standard chow and §P < 0.05 in AAB groups vs. respective sham controls. Group sizes as indicated on individual bars.
Mentions: PDH flux was determined in vivo through incorporation of the hyperpolarized 13C label from [1-13C]pyruvate into bicarbonate as previously demonstrated21 (Figure 5). Rates of 13C label incorporation into bicarbonate in AAB groups at the different time points were not significantly different from their respective controls (Figure 6A), implying little effect of AAB on PDH flux in vivo and thus glucose oxidation over the time course of hypertrophic development. At all time points however, dietary manipulation resulted in a marked reduction in 13C label incorporation into bicarbonate, indicating that PDH flux was reduced in the WD groups compared with their respective standard chow groups as would be predicted from the Randle cycle.26

Bottom Line: Pyruvate dehydrogenase flux was unchanged in the hypertrophied animals at any time point, but increased incorporation of the (13)C label into lactate was observed by 9 weeks and maintained at 14 weeks, indicative of enhanced glycolysis.Hypertrophied hearts revealed little evidence of a switch towards increased glucose oxidation but rather an uncoupling of glycolytic metabolism from glucose oxidation.This was maintained under conditions of dietary stress provided by a WD but, at this compensated phase of hypertrophy, did not result in any contractile dysfunction.

View Article: PubMed Central - PubMed

Affiliation: School of Biological, Biomedical and Environmental Sciences, University of Hull, Hull HU6 7RX, UK.

No MeSH data available.


Related in: MedlinePlus