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In vivo assessment of cardiac metabolism and function in the abdominal aortic banding model of compensated cardiac hypertrophy.

Seymour AM, Giles L, Ball V, Miller JJ, Clarke K, Carr CA, Tyler DJ - Cardiovasc. Res. (2015)

Bottom Line: Pyruvate dehydrogenase flux was unchanged in the hypertrophied animals at any time point, but increased incorporation of the (13)C label into lactate was observed by 9 weeks and maintained at 14 weeks, indicative of enhanced glycolysis.Hypertrophied hearts revealed little evidence of a switch towards increased glucose oxidation but rather an uncoupling of glycolytic metabolism from glucose oxidation.This was maintained under conditions of dietary stress provided by a WD but, at this compensated phase of hypertrophy, did not result in any contractile dysfunction.

View Article: PubMed Central - PubMed

Affiliation: School of Biological, Biomedical and Environmental Sciences, University of Hull, Hull HU6 7RX, UK.

No MeSH data available.


Related in: MedlinePlus

Functional characteristics of control and AAB hearts in vivo. (A) Ejection Fraction, (B) stroke volume, (C) cardiac output, and (D) cardiac index in sham and AAB animals at 4, 9, or 14 weeks post-surgical induction of cardiac hypertrophy, exposed to standard chow or WD. *P < 0.05 in WD groups compared with standard chow groups and §P < 0.05 in AAB groups compared with sham control groups. Group sizes as indicated on individual bars.
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Figure 4: Functional characteristics of control and AAB hearts in vivo. (A) Ejection Fraction, (B) stroke volume, (C) cardiac output, and (D) cardiac index in sham and AAB animals at 4, 9, or 14 weeks post-surgical induction of cardiac hypertrophy, exposed to standard chow or WD. *P < 0.05 in WD groups compared with standard chow groups and §P < 0.05 in AAB groups compared with sham control groups. Group sizes as indicated on individual bars.

Mentions: Initially systolic and diastolic volumes were enlarged in AAB groups at 4 weeks post induction of hypertrophy (Figure 3), which resulted in a reduction in ejection fraction (Figure 4). However by 14 weeks, these alterations had resolved, indicating a transition from an initial acute phase of hypertrophic induction to a more compensated stage. End-systolic volumes were reduced in the WD-fed animals at the 4-week time point, resulting in an elevated ejection fraction; however, this also resolved with time and no structural differences were seen between chow and WD-fed animals at 14 weeks (Figures 3 and 4).


In vivo assessment of cardiac metabolism and function in the abdominal aortic banding model of compensated cardiac hypertrophy.

Seymour AM, Giles L, Ball V, Miller JJ, Clarke K, Carr CA, Tyler DJ - Cardiovasc. Res. (2015)

Functional characteristics of control and AAB hearts in vivo. (A) Ejection Fraction, (B) stroke volume, (C) cardiac output, and (D) cardiac index in sham and AAB animals at 4, 9, or 14 weeks post-surgical induction of cardiac hypertrophy, exposed to standard chow or WD. *P < 0.05 in WD groups compared with standard chow groups and §P < 0.05 in AAB groups compared with sham control groups. Group sizes as indicated on individual bars.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400188&req=5

Figure 4: Functional characteristics of control and AAB hearts in vivo. (A) Ejection Fraction, (B) stroke volume, (C) cardiac output, and (D) cardiac index in sham and AAB animals at 4, 9, or 14 weeks post-surgical induction of cardiac hypertrophy, exposed to standard chow or WD. *P < 0.05 in WD groups compared with standard chow groups and §P < 0.05 in AAB groups compared with sham control groups. Group sizes as indicated on individual bars.
Mentions: Initially systolic and diastolic volumes were enlarged in AAB groups at 4 weeks post induction of hypertrophy (Figure 3), which resulted in a reduction in ejection fraction (Figure 4). However by 14 weeks, these alterations had resolved, indicating a transition from an initial acute phase of hypertrophic induction to a more compensated stage. End-systolic volumes were reduced in the WD-fed animals at the 4-week time point, resulting in an elevated ejection fraction; however, this also resolved with time and no structural differences were seen between chow and WD-fed animals at 14 weeks (Figures 3 and 4).

Bottom Line: Pyruvate dehydrogenase flux was unchanged in the hypertrophied animals at any time point, but increased incorporation of the (13)C label into lactate was observed by 9 weeks and maintained at 14 weeks, indicative of enhanced glycolysis.Hypertrophied hearts revealed little evidence of a switch towards increased glucose oxidation but rather an uncoupling of glycolytic metabolism from glucose oxidation.This was maintained under conditions of dietary stress provided by a WD but, at this compensated phase of hypertrophy, did not result in any contractile dysfunction.

View Article: PubMed Central - PubMed

Affiliation: School of Biological, Biomedical and Environmental Sciences, University of Hull, Hull HU6 7RX, UK.

No MeSH data available.


Related in: MedlinePlus