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In vivo assessment of cardiac metabolism and function in the abdominal aortic banding model of compensated cardiac hypertrophy.

Seymour AM, Giles L, Ball V, Miller JJ, Clarke K, Carr CA, Tyler DJ - Cardiovasc. Res. (2015)

Bottom Line: Pyruvate dehydrogenase flux was unchanged in the hypertrophied animals at any time point, but increased incorporation of the (13)C label into lactate was observed by 9 weeks and maintained at 14 weeks, indicative of enhanced glycolysis.Hypertrophied hearts revealed little evidence of a switch towards increased glucose oxidation but rather an uncoupling of glycolytic metabolism from glucose oxidation.This was maintained under conditions of dietary stress provided by a WD but, at this compensated phase of hypertrophy, did not result in any contractile dysfunction.

View Article: PubMed Central - PubMed

Affiliation: School of Biological, Biomedical and Environmental Sciences, University of Hull, Hull HU6 7RX, UK.

No MeSH data available.


Related in: MedlinePlus

Cardiac structural parameters assessed in control and AAB animals at 4, 9, and 14 weeks post-surgical induction of cardiac hypertrophy. (A) LVM, (B) heart weight to body weight ratio, (C) end-diastolic volume, and (D) end-systolic volume. *P < 0.05 in WD groups compared with standard chow groups and §P < 0.05 in AAB groups compared with sham control groups. Group sizes as indicated on individual bars.
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Figure 3: Cardiac structural parameters assessed in control and AAB animals at 4, 9, and 14 weeks post-surgical induction of cardiac hypertrophy. (A) LVM, (B) heart weight to body weight ratio, (C) end-diastolic volume, and (D) end-systolic volume. *P < 0.05 in WD groups compared with standard chow groups and §P < 0.05 in AAB groups compared with sham control groups. Group sizes as indicated on individual bars.

Mentions: In vivo CINE MRI was used to study the extent of structural remodelling in hearts of animals subjected to AAB over time. Figure 2 shows representative images of hypertrophied and control hearts in systole and diastole after 14 weeks of exposure to standard chow (Figure 2A and B) or WD (Figure 2C and D) with evident enlargement of the ventricle in AAB hearts independent of any dietary modification. Quantitatively, left ventricular mass (LVM) was markedly augmented in the AAB group compared with its respective control by 4 weeks post surgery (17%) and this increase was maintained over 9 (14%) and 14 weeks (14%), irrespective of dietary manipulation (Figure 3A). This was further reflected in the hypertrophic index of LVM to body weight ratio (Figure 3B), indicating that the hypertrophy occurred primarily as a result of the hypertension induced by aortic banding rather than any effect of the diet.


In vivo assessment of cardiac metabolism and function in the abdominal aortic banding model of compensated cardiac hypertrophy.

Seymour AM, Giles L, Ball V, Miller JJ, Clarke K, Carr CA, Tyler DJ - Cardiovasc. Res. (2015)

Cardiac structural parameters assessed in control and AAB animals at 4, 9, and 14 weeks post-surgical induction of cardiac hypertrophy. (A) LVM, (B) heart weight to body weight ratio, (C) end-diastolic volume, and (D) end-systolic volume. *P < 0.05 in WD groups compared with standard chow groups and §P < 0.05 in AAB groups compared with sham control groups. Group sizes as indicated on individual bars.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400188&req=5

Figure 3: Cardiac structural parameters assessed in control and AAB animals at 4, 9, and 14 weeks post-surgical induction of cardiac hypertrophy. (A) LVM, (B) heart weight to body weight ratio, (C) end-diastolic volume, and (D) end-systolic volume. *P < 0.05 in WD groups compared with standard chow groups and §P < 0.05 in AAB groups compared with sham control groups. Group sizes as indicated on individual bars.
Mentions: In vivo CINE MRI was used to study the extent of structural remodelling in hearts of animals subjected to AAB over time. Figure 2 shows representative images of hypertrophied and control hearts in systole and diastole after 14 weeks of exposure to standard chow (Figure 2A and B) or WD (Figure 2C and D) with evident enlargement of the ventricle in AAB hearts independent of any dietary modification. Quantitatively, left ventricular mass (LVM) was markedly augmented in the AAB group compared with its respective control by 4 weeks post surgery (17%) and this increase was maintained over 9 (14%) and 14 weeks (14%), irrespective of dietary manipulation (Figure 3A). This was further reflected in the hypertrophic index of LVM to body weight ratio (Figure 3B), indicating that the hypertrophy occurred primarily as a result of the hypertension induced by aortic banding rather than any effect of the diet.

Bottom Line: Pyruvate dehydrogenase flux was unchanged in the hypertrophied animals at any time point, but increased incorporation of the (13)C label into lactate was observed by 9 weeks and maintained at 14 weeks, indicative of enhanced glycolysis.Hypertrophied hearts revealed little evidence of a switch towards increased glucose oxidation but rather an uncoupling of glycolytic metabolism from glucose oxidation.This was maintained under conditions of dietary stress provided by a WD but, at this compensated phase of hypertrophy, did not result in any contractile dysfunction.

View Article: PubMed Central - PubMed

Affiliation: School of Biological, Biomedical and Environmental Sciences, University of Hull, Hull HU6 7RX, UK.

No MeSH data available.


Related in: MedlinePlus