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Transcutaneous vagus nerve stimulation induces tidal melatonin secretion and has an antidiabetic effect in Zucker fatty rats.

Wang S, Zhai X, Li S, McCabe MF, Wang X, Rong P - PLoS ONE (2015)

Bottom Line: We found that naïve ZDF rats develop hyperglycemia naturally with age.Once daily taVNS sessions eventually reduced the glucose concentration to a normal level in seven days and effectively maintained the normal glycemic and plasma glycosylated hemoglobin (HbAlc) levels when applied for five consecutive weeks.We concluded that multiple taVNS sessions are antidiabetic in T2D through triggering of tidal secretion of melatonin.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Xinxiang Medical University, Xinxiang, Henan Province, China; Department of Physiology, Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing, China; MGH Center for Translational Pain Research, Department of Anesthesia, Critical Care, and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, United States of America; Guangdong Landau Biotechnology Inc. Ltd., Guangzhou, Guangdong, China.

ABSTRACT
Melatonin plays a protective role in type 2 diabetes (T2D) through regulation of glucose metabolism. Whether transcutaneous vagus nerve stimulation (taVNS) is antidiabetic and whether a modulated melatonin production is involved in the antidiabetic mechanism of taVNS is unknown. In this study, once daily 30 min noninvasive taVNS was administered in Zucker diabetic fatty (ZDF, fa/fa) and Zucker lean (ZL, +/fa) littermates under anesthesia for 5 consecutive weeks. The acute and chronic influences of taVNS on the secretion of melatonin were studied as well as the effects of taVNS on blood glucose metabolism. We found that naïve ZDF rats develop hyperglycemia naturally with age. Each taVNS session would trigger a tidal secretion of melatonin both during and after the taVNS procedure and induce an acute two-phase glycemic change, a steep increase followed by a gradual decrease. Once daily taVNS sessions eventually reduced the glucose concentration to a normal level in seven days and effectively maintained the normal glycemic and plasma glycosylated hemoglobin (HbAlc) levels when applied for five consecutive weeks. These beneficial effects of taVNS also exist in pinealectomized rats, which otherwise would show overt and continuous hyperglycemia, hyperinsulinemia, and high HbAlc levels. We concluded that multiple taVNS sessions are antidiabetic in T2D through triggering of tidal secretion of melatonin. This finding may have potential importance in developing new approaches to the treatment of T2D, which is highly prevalent, incurable with any current approaches, and very costly to the world.

No MeSH data available.


Related in: MedlinePlus

The electroacupuncture procedure.Showing the auricular stimulation posture (a) and location (b) of the procedure. AMEA: stimulation applied at auricular margin; taVNS: stimulation applied at auricular concha.
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pone.0124195.g001: The electroacupuncture procedure.Showing the auricular stimulation posture (a) and location (b) of the procedure. AMEA: stimulation applied at auricular margin; taVNS: stimulation applied at auricular concha.

Mentions: All the time points recorded in this study are in accordance with the taVNS occurrences, i.e. the first taVNS session occurs on day 1, the seventh day recorded as W1, and so on. For taVNS, under 2% isoflurane inhalation anesthesia, two oppositely charged magnetic electrodes (+/-) were placed over the auricular concha region, inside and outside respectively, of each ear. Saline was applied between an electrode and the skin to improve electric conductivity. A session of 30min transcutaneous electrical stimulation at frequencies of 2/15 Hz (2 and 15 Hz, switched every second) and an intensity of 2mA was applied via an electrical stimulator (HANS-100, Nanjing, China). The procedures were given in the afternoon after a blood glucose concentration test and a blood sample collection at designated time points. Auricular margin was used as the sham acupoint. The electroacupuncture condition at auricular margin was same as that at taVNS except the stimulate location (Fig 1).


Transcutaneous vagus nerve stimulation induces tidal melatonin secretion and has an antidiabetic effect in Zucker fatty rats.

Wang S, Zhai X, Li S, McCabe MF, Wang X, Rong P - PLoS ONE (2015)

The electroacupuncture procedure.Showing the auricular stimulation posture (a) and location (b) of the procedure. AMEA: stimulation applied at auricular margin; taVNS: stimulation applied at auricular concha.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400163&req=5

pone.0124195.g001: The electroacupuncture procedure.Showing the auricular stimulation posture (a) and location (b) of the procedure. AMEA: stimulation applied at auricular margin; taVNS: stimulation applied at auricular concha.
Mentions: All the time points recorded in this study are in accordance with the taVNS occurrences, i.e. the first taVNS session occurs on day 1, the seventh day recorded as W1, and so on. For taVNS, under 2% isoflurane inhalation anesthesia, two oppositely charged magnetic electrodes (+/-) were placed over the auricular concha region, inside and outside respectively, of each ear. Saline was applied between an electrode and the skin to improve electric conductivity. A session of 30min transcutaneous electrical stimulation at frequencies of 2/15 Hz (2 and 15 Hz, switched every second) and an intensity of 2mA was applied via an electrical stimulator (HANS-100, Nanjing, China). The procedures were given in the afternoon after a blood glucose concentration test and a blood sample collection at designated time points. Auricular margin was used as the sham acupoint. The electroacupuncture condition at auricular margin was same as that at taVNS except the stimulate location (Fig 1).

Bottom Line: We found that naïve ZDF rats develop hyperglycemia naturally with age.Once daily taVNS sessions eventually reduced the glucose concentration to a normal level in seven days and effectively maintained the normal glycemic and plasma glycosylated hemoglobin (HbAlc) levels when applied for five consecutive weeks.We concluded that multiple taVNS sessions are antidiabetic in T2D through triggering of tidal secretion of melatonin.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Xinxiang Medical University, Xinxiang, Henan Province, China; Department of Physiology, Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing, China; MGH Center for Translational Pain Research, Department of Anesthesia, Critical Care, and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, United States of America; Guangdong Landau Biotechnology Inc. Ltd., Guangzhou, Guangdong, China.

ABSTRACT
Melatonin plays a protective role in type 2 diabetes (T2D) through regulation of glucose metabolism. Whether transcutaneous vagus nerve stimulation (taVNS) is antidiabetic and whether a modulated melatonin production is involved in the antidiabetic mechanism of taVNS is unknown. In this study, once daily 30 min noninvasive taVNS was administered in Zucker diabetic fatty (ZDF, fa/fa) and Zucker lean (ZL, +/fa) littermates under anesthesia for 5 consecutive weeks. The acute and chronic influences of taVNS on the secretion of melatonin were studied as well as the effects of taVNS on blood glucose metabolism. We found that naïve ZDF rats develop hyperglycemia naturally with age. Each taVNS session would trigger a tidal secretion of melatonin both during and after the taVNS procedure and induce an acute two-phase glycemic change, a steep increase followed by a gradual decrease. Once daily taVNS sessions eventually reduced the glucose concentration to a normal level in seven days and effectively maintained the normal glycemic and plasma glycosylated hemoglobin (HbAlc) levels when applied for five consecutive weeks. These beneficial effects of taVNS also exist in pinealectomized rats, which otherwise would show overt and continuous hyperglycemia, hyperinsulinemia, and high HbAlc levels. We concluded that multiple taVNS sessions are antidiabetic in T2D through triggering of tidal secretion of melatonin. This finding may have potential importance in developing new approaches to the treatment of T2D, which is highly prevalent, incurable with any current approaches, and very costly to the world.

No MeSH data available.


Related in: MedlinePlus