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A new synthetic allotetraploid (A1A1G2G2) between Gossypium herbaceum and G. australe: bridging for simultaneously transferring favorable genes from these two diploid species into upland cotton.

Liu Q, Chen Y, Chen Y, Wang Y, Chen J, Zhang T, Zhou B - PLoS ONE (2015)

Bottom Line: Creating synthetic allotetraploid cotton from these two species would lay the foundation for simultaneously transferring favorable genes into cultivated tetraploid cotton.Here, we crossed G. herbaceum (as the maternal parent) with G. australe to produce an F1 interspecific hybrid and doubled its chromosome complement with colchicine, successfully generating a synthetic tetraploid.The synthetic allotetraploid will be quite useful for polyploidy evolutionary studies and as a bridge for transferring favorable genes from these two diploid species into Upland cotton through hybridization.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Genetics & Germplasm Enhancement, MOE Hybrid Cotton R&D Engineering Research Center, Nanjing Agricultural University, Nanjing, Jiangsu, People's Republic of China.

ABSTRACT
Gossypium herbaceum, a cultivated diploid cotton species (2n = 2x = 26, A1A1), has favorable traits such as excellent drought tolerance and resistance to sucking insects and leaf curl virus. G. australe, a wild diploid cotton species (2n = 2x = 26, G2G2), possesses numerous economically valuable characteristics such as delayed pigment gland morphogenesis (which is conducive to the production of seeds with very low levels of gossypol as a potential food source for humans and animals) and resistance to insects, wilt diseases and abiotic stress. Creating synthetic allotetraploid cotton from these two species would lay the foundation for simultaneously transferring favorable genes into cultivated tetraploid cotton. Here, we crossed G. herbaceum (as the maternal parent) with G. australe to produce an F1 interspecific hybrid and doubled its chromosome complement with colchicine, successfully generating a synthetic tetraploid. The obtained tetraploid was confirmed by morphology, cytology and molecular markers and then self-pollinated. The S1 seedlings derived from this tetraploid gradually became flavescent after emergence of the fifth true leaf, but they were rescued by grafting and produced S2 seeds. The rescued S1 plants were partially fertile due to the existence of univalents at Metaphase I of meiosis, leading to the formation of unbalanced, nonviable gametes lacking complete sets of chromosomes. The S2 plants grew well and no flavescence was observed, implying that interspecific incompatibility, to some extent, had been alleviated in the S2 generation. The synthetic allotetraploid will be quite useful for polyploidy evolutionary studies and as a bridge for transferring favorable genes from these two diploid species into Upland cotton through hybridization.

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Related in: MedlinePlus

Grafted S1 plant (left) and non-grafted S2 seedling (right) grew normally.The arrow indicates the grafted part of the plant shown on the left.
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pone.0123209.g001: Grafted S1 plant (left) and non-grafted S2 seedling (right) grew normally.The arrow indicates the grafted part of the plant shown on the left.

Mentions: In 2014, we planted three S2 seeds on MS medium in a triangular flask to collect healthy root tips for cytological observation. The seeds germinated normally, and root tips were collected. These S2 plants grew well into the squaring stage (Fig 1). No flavescent lethal phenomenon occurred during the development of S2 plants, which implies that interspecific incompatibility, to some extent, had been alleviated in the S2 generation. The above results suggest that both S1 and S2 were new synthetic allotetraploid plants.


A new synthetic allotetraploid (A1A1G2G2) between Gossypium herbaceum and G. australe: bridging for simultaneously transferring favorable genes from these two diploid species into upland cotton.

Liu Q, Chen Y, Chen Y, Wang Y, Chen J, Zhang T, Zhou B - PLoS ONE (2015)

Grafted S1 plant (left) and non-grafted S2 seedling (right) grew normally.The arrow indicates the grafted part of the plant shown on the left.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400159&req=5

pone.0123209.g001: Grafted S1 plant (left) and non-grafted S2 seedling (right) grew normally.The arrow indicates the grafted part of the plant shown on the left.
Mentions: In 2014, we planted three S2 seeds on MS medium in a triangular flask to collect healthy root tips for cytological observation. The seeds germinated normally, and root tips were collected. These S2 plants grew well into the squaring stage (Fig 1). No flavescent lethal phenomenon occurred during the development of S2 plants, which implies that interspecific incompatibility, to some extent, had been alleviated in the S2 generation. The above results suggest that both S1 and S2 were new synthetic allotetraploid plants.

Bottom Line: Creating synthetic allotetraploid cotton from these two species would lay the foundation for simultaneously transferring favorable genes into cultivated tetraploid cotton.Here, we crossed G. herbaceum (as the maternal parent) with G. australe to produce an F1 interspecific hybrid and doubled its chromosome complement with colchicine, successfully generating a synthetic tetraploid.The synthetic allotetraploid will be quite useful for polyploidy evolutionary studies and as a bridge for transferring favorable genes from these two diploid species into Upland cotton through hybridization.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Genetics & Germplasm Enhancement, MOE Hybrid Cotton R&D Engineering Research Center, Nanjing Agricultural University, Nanjing, Jiangsu, People's Republic of China.

ABSTRACT
Gossypium herbaceum, a cultivated diploid cotton species (2n = 2x = 26, A1A1), has favorable traits such as excellent drought tolerance and resistance to sucking insects and leaf curl virus. G. australe, a wild diploid cotton species (2n = 2x = 26, G2G2), possesses numerous economically valuable characteristics such as delayed pigment gland morphogenesis (which is conducive to the production of seeds with very low levels of gossypol as a potential food source for humans and animals) and resistance to insects, wilt diseases and abiotic stress. Creating synthetic allotetraploid cotton from these two species would lay the foundation for simultaneously transferring favorable genes into cultivated tetraploid cotton. Here, we crossed G. herbaceum (as the maternal parent) with G. australe to produce an F1 interspecific hybrid and doubled its chromosome complement with colchicine, successfully generating a synthetic tetraploid. The obtained tetraploid was confirmed by morphology, cytology and molecular markers and then self-pollinated. The S1 seedlings derived from this tetraploid gradually became flavescent after emergence of the fifth true leaf, but they were rescued by grafting and produced S2 seeds. The rescued S1 plants were partially fertile due to the existence of univalents at Metaphase I of meiosis, leading to the formation of unbalanced, nonviable gametes lacking complete sets of chromosomes. The S2 plants grew well and no flavescence was observed, implying that interspecific incompatibility, to some extent, had been alleviated in the S2 generation. The synthetic allotetraploid will be quite useful for polyploidy evolutionary studies and as a bridge for transferring favorable genes from these two diploid species into Upland cotton through hybridization.

Show MeSH
Related in: MedlinePlus