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Molecular survey of bacterial communities associated with bacterial chondronecrosis with osteomyelitis (BCO) in broilers.

Jiang T, Mandal RK, Wideman RF, Khatiwara A, Pevzner I, Min Kwon Y - PLoS ONE (2015)

Bottom Line: Relatively little is known about the microbial communities associated with BCO.Rarefaction analysis demonstrated the general tendency that increased severities of BCO lesions were associated with reduced species diversity in both femoral and tibial samples when compared to macroscopically normal samples.These observations suggest that certain bacterial subgroups are preferentially selected in association with the development of BCO lesions.

View Article: PubMed Central - PubMed

Affiliation: Department of Poultry Science, University of Arkansas, Fayetteville, Arkansas, United States of America.

ABSTRACT
Bacterial chondronecrosis with osteomyelitis (BCO) is recognized as an important cause of lameness in commercial broiler chickens (meat-type chickens). Relatively little is known about the microbial communities associated with BCO. This study was conducted to increase our understanding of the microbial factors associated with BCO using a culture-independent approach. Using Illumina sequencing of the hyper-variable region V6 in the 16S rRNA gene, we characterized the bacterial communities in 97 femoral or tibial heads from normal and lame broilers carefully selected to represent diverse variations in age, line, lesion type, floor type, clinical status and bone type. Our in-depth survey based on 14 million assembled sequence reads revealed that complex bacterial communities exist in all samples, including macroscopically normal bones from clinically healthy birds. Overall, Proteobacteria (mean 90.9%) comprised the most common phylum, followed by Firmicutes (6.1%) and Actinobacteria (2.6%), accounting for more than 99% of all reads. Statistical analyses demonstrated that there are differences in bacterial communities in different types of bones (femur vs. tibia), lesion types (macroscopically normal femora or tibiae vs. those with pathognomonic BCO lesions), and among individual birds. This analysis also showed that BCO samples overrepresented genera Staphylococcus, whose species have been frequently isolated in BCO samples in previous studies. Rarefaction analysis demonstrated the general tendency that increased severities of BCO lesions were associated with reduced species diversity in both femoral and tibial samples when compared to macroscopically normal samples. These observations suggest that certain bacterial subgroups are preferentially selected in association with the development of BCO lesions. Understanding the microbial species associated with BCO will identify opportunities for understanding and modulating the pathogenesis of this form of lameness in broilers.

No MeSH data available.


Related in: MedlinePlus

Taxonomic composition of the bone samples based at genus level.Only 15 major genera of significant importance were selected and shown in the graphs.
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pone.0124403.g009: Taxonomic composition of the bone samples based at genus level.Only 15 major genera of significant importance were selected and shown in the graphs.

Mentions: The community structure of femor associated bacteria differed significantly from tibial bacteria assemblages (Table 1). The observed difference may be due to the changes in physiological micro-environments caused by the different torque and shear stresses imposed on the femoral vs. tibial joints. For this analysis we selected 51 femur and 46 tibia samples (S1 Table). The results of taxonomic analysis of the V6 16S rRNA gene amplicon reads show that there are a total of 9 phyla across both bone locations, with the dominant phylum being Proteobacteria (mean 89.7% for the femur and 91.6% for the tibia) and followed by Firmicutes (6.8% for the femur and 5.7% for the tibia) and Actinobacteria (2.9% for the femur and 2.3% for the tibia), accounting for more 99% of all reads (Fig 4). We also compared the changes in bacterial communities in both femur and tibia at genus level. Fig 9 shows the distribution of major genera that belong to either Proteobacteria or Firmicutes. For the normal bones, there was a striking similarity in the changing patterns of the major genera over time between the femur and tibia. On the contrast, however, the development of the patterns was very distinctive between the femur and tibia with BCO lesions. It is interesting that the abundance of the genera Serratia and Vibrio increased to nearly 80% of total bacterial population at the later stages for THN and THNsc, respectively.


Molecular survey of bacterial communities associated with bacterial chondronecrosis with osteomyelitis (BCO) in broilers.

Jiang T, Mandal RK, Wideman RF, Khatiwara A, Pevzner I, Min Kwon Y - PLoS ONE (2015)

Taxonomic composition of the bone samples based at genus level.Only 15 major genera of significant importance were selected and shown in the graphs.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400152&req=5

pone.0124403.g009: Taxonomic composition of the bone samples based at genus level.Only 15 major genera of significant importance were selected and shown in the graphs.
Mentions: The community structure of femor associated bacteria differed significantly from tibial bacteria assemblages (Table 1). The observed difference may be due to the changes in physiological micro-environments caused by the different torque and shear stresses imposed on the femoral vs. tibial joints. For this analysis we selected 51 femur and 46 tibia samples (S1 Table). The results of taxonomic analysis of the V6 16S rRNA gene amplicon reads show that there are a total of 9 phyla across both bone locations, with the dominant phylum being Proteobacteria (mean 89.7% for the femur and 91.6% for the tibia) and followed by Firmicutes (6.8% for the femur and 5.7% for the tibia) and Actinobacteria (2.9% for the femur and 2.3% for the tibia), accounting for more 99% of all reads (Fig 4). We also compared the changes in bacterial communities in both femur and tibia at genus level. Fig 9 shows the distribution of major genera that belong to either Proteobacteria or Firmicutes. For the normal bones, there was a striking similarity in the changing patterns of the major genera over time between the femur and tibia. On the contrast, however, the development of the patterns was very distinctive between the femur and tibia with BCO lesions. It is interesting that the abundance of the genera Serratia and Vibrio increased to nearly 80% of total bacterial population at the later stages for THN and THNsc, respectively.

Bottom Line: Relatively little is known about the microbial communities associated with BCO.Rarefaction analysis demonstrated the general tendency that increased severities of BCO lesions were associated with reduced species diversity in both femoral and tibial samples when compared to macroscopically normal samples.These observations suggest that certain bacterial subgroups are preferentially selected in association with the development of BCO lesions.

View Article: PubMed Central - PubMed

Affiliation: Department of Poultry Science, University of Arkansas, Fayetteville, Arkansas, United States of America.

ABSTRACT
Bacterial chondronecrosis with osteomyelitis (BCO) is recognized as an important cause of lameness in commercial broiler chickens (meat-type chickens). Relatively little is known about the microbial communities associated with BCO. This study was conducted to increase our understanding of the microbial factors associated with BCO using a culture-independent approach. Using Illumina sequencing of the hyper-variable region V6 in the 16S rRNA gene, we characterized the bacterial communities in 97 femoral or tibial heads from normal and lame broilers carefully selected to represent diverse variations in age, line, lesion type, floor type, clinical status and bone type. Our in-depth survey based on 14 million assembled sequence reads revealed that complex bacterial communities exist in all samples, including macroscopically normal bones from clinically healthy birds. Overall, Proteobacteria (mean 90.9%) comprised the most common phylum, followed by Firmicutes (6.1%) and Actinobacteria (2.6%), accounting for more than 99% of all reads. Statistical analyses demonstrated that there are differences in bacterial communities in different types of bones (femur vs. tibia), lesion types (macroscopically normal femora or tibiae vs. those with pathognomonic BCO lesions), and among individual birds. This analysis also showed that BCO samples overrepresented genera Staphylococcus, whose species have been frequently isolated in BCO samples in previous studies. Rarefaction analysis demonstrated the general tendency that increased severities of BCO lesions were associated with reduced species diversity in both femoral and tibial samples when compared to macroscopically normal samples. These observations suggest that certain bacterial subgroups are preferentially selected in association with the development of BCO lesions. Understanding the microbial species associated with BCO will identify opportunities for understanding and modulating the pathogenesis of this form of lameness in broilers.

No MeSH data available.


Related in: MedlinePlus