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Molecular survey of bacterial communities associated with bacterial chondronecrosis with osteomyelitis (BCO) in broilers.

Jiang T, Mandal RK, Wideman RF, Khatiwara A, Pevzner I, Min Kwon Y - PLoS ONE (2015)

Bottom Line: Relatively little is known about the microbial communities associated with BCO.Rarefaction analysis demonstrated the general tendency that increased severities of BCO lesions were associated with reduced species diversity in both femoral and tibial samples when compared to macroscopically normal samples.These observations suggest that certain bacterial subgroups are preferentially selected in association with the development of BCO lesions.

View Article: PubMed Central - PubMed

Affiliation: Department of Poultry Science, University of Arkansas, Fayetteville, Arkansas, United States of America.

ABSTRACT
Bacterial chondronecrosis with osteomyelitis (BCO) is recognized as an important cause of lameness in commercial broiler chickens (meat-type chickens). Relatively little is known about the microbial communities associated with BCO. This study was conducted to increase our understanding of the microbial factors associated with BCO using a culture-independent approach. Using Illumina sequencing of the hyper-variable region V6 in the 16S rRNA gene, we characterized the bacterial communities in 97 femoral or tibial heads from normal and lame broilers carefully selected to represent diverse variations in age, line, lesion type, floor type, clinical status and bone type. Our in-depth survey based on 14 million assembled sequence reads revealed that complex bacterial communities exist in all samples, including macroscopically normal bones from clinically healthy birds. Overall, Proteobacteria (mean 90.9%) comprised the most common phylum, followed by Firmicutes (6.1%) and Actinobacteria (2.6%), accounting for more than 99% of all reads. Statistical analyses demonstrated that there are differences in bacterial communities in different types of bones (femur vs. tibia), lesion types (macroscopically normal femora or tibiae vs. those with pathognomonic BCO lesions), and among individual birds. This analysis also showed that BCO samples overrepresented genera Staphylococcus, whose species have been frequently isolated in BCO samples in previous studies. Rarefaction analysis demonstrated the general tendency that increased severities of BCO lesions were associated with reduced species diversity in both femoral and tibial samples when compared to macroscopically normal samples. These observations suggest that certain bacterial subgroups are preferentially selected in association with the development of BCO lesions. Understanding the microbial species associated with BCO will identify opportunities for understanding and modulating the pathogenesis of this form of lameness in broilers.

No MeSH data available.


Related in: MedlinePlus

Hierarchical clustering analysis of the bacterial communities.Phylogenetic tree shows that most of FHS (dark blue), THN (green), FHN (pink) and THNsc (red) samples can be clustered. Femur Normal (black), and Tibia Normal (light blue) were largely mixed together in two different clusters.
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pone.0124403.g006: Hierarchical clustering analysis of the bacterial communities.Phylogenetic tree shows that most of FHS (dark blue), THN (green), FHN (pink) and THNsc (red) samples can be clustered. Femur Normal (black), and Tibia Normal (light blue) were largely mixed together in two different clusters.

Mentions: Hierarchical clustering of the bacterial communities in our 6 BCO diagnostic categories (Normal Femur, FHS, FHN, Normal Tibia, THN and THNsc) was conducted by using the default β diversity metrics of un-weighted UniFrac. The resulting phylogenetic tree shows that most of the FHS, THN, FHN and THNsc samples tended to cluster together within a diagnostic category (Fig 6). The same phylogenetic tree also demonstrates that the Normal femur and Normal tibia samples grouped together in two different clusters that essentially were segregated from the BCO lesion samples. Evidently no distinctive difference existed between these “normal” sample groups from the two different bone types (femur and tibia) (p>0.05).


Molecular survey of bacterial communities associated with bacterial chondronecrosis with osteomyelitis (BCO) in broilers.

Jiang T, Mandal RK, Wideman RF, Khatiwara A, Pevzner I, Min Kwon Y - PLoS ONE (2015)

Hierarchical clustering analysis of the bacterial communities.Phylogenetic tree shows that most of FHS (dark blue), THN (green), FHN (pink) and THNsc (red) samples can be clustered. Femur Normal (black), and Tibia Normal (light blue) were largely mixed together in two different clusters.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400152&req=5

pone.0124403.g006: Hierarchical clustering analysis of the bacterial communities.Phylogenetic tree shows that most of FHS (dark blue), THN (green), FHN (pink) and THNsc (red) samples can be clustered. Femur Normal (black), and Tibia Normal (light blue) were largely mixed together in two different clusters.
Mentions: Hierarchical clustering of the bacterial communities in our 6 BCO diagnostic categories (Normal Femur, FHS, FHN, Normal Tibia, THN and THNsc) was conducted by using the default β diversity metrics of un-weighted UniFrac. The resulting phylogenetic tree shows that most of the FHS, THN, FHN and THNsc samples tended to cluster together within a diagnostic category (Fig 6). The same phylogenetic tree also demonstrates that the Normal femur and Normal tibia samples grouped together in two different clusters that essentially were segregated from the BCO lesion samples. Evidently no distinctive difference existed between these “normal” sample groups from the two different bone types (femur and tibia) (p>0.05).

Bottom Line: Relatively little is known about the microbial communities associated with BCO.Rarefaction analysis demonstrated the general tendency that increased severities of BCO lesions were associated with reduced species diversity in both femoral and tibial samples when compared to macroscopically normal samples.These observations suggest that certain bacterial subgroups are preferentially selected in association with the development of BCO lesions.

View Article: PubMed Central - PubMed

Affiliation: Department of Poultry Science, University of Arkansas, Fayetteville, Arkansas, United States of America.

ABSTRACT
Bacterial chondronecrosis with osteomyelitis (BCO) is recognized as an important cause of lameness in commercial broiler chickens (meat-type chickens). Relatively little is known about the microbial communities associated with BCO. This study was conducted to increase our understanding of the microbial factors associated with BCO using a culture-independent approach. Using Illumina sequencing of the hyper-variable region V6 in the 16S rRNA gene, we characterized the bacterial communities in 97 femoral or tibial heads from normal and lame broilers carefully selected to represent diverse variations in age, line, lesion type, floor type, clinical status and bone type. Our in-depth survey based on 14 million assembled sequence reads revealed that complex bacterial communities exist in all samples, including macroscopically normal bones from clinically healthy birds. Overall, Proteobacteria (mean 90.9%) comprised the most common phylum, followed by Firmicutes (6.1%) and Actinobacteria (2.6%), accounting for more than 99% of all reads. Statistical analyses demonstrated that there are differences in bacterial communities in different types of bones (femur vs. tibia), lesion types (macroscopically normal femora or tibiae vs. those with pathognomonic BCO lesions), and among individual birds. This analysis also showed that BCO samples overrepresented genera Staphylococcus, whose species have been frequently isolated in BCO samples in previous studies. Rarefaction analysis demonstrated the general tendency that increased severities of BCO lesions were associated with reduced species diversity in both femoral and tibial samples when compared to macroscopically normal samples. These observations suggest that certain bacterial subgroups are preferentially selected in association with the development of BCO lesions. Understanding the microbial species associated with BCO will identify opportunities for understanding and modulating the pathogenesis of this form of lameness in broilers.

No MeSH data available.


Related in: MedlinePlus