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Granulocytic myeloid derived suppressor cells expansion during active pulmonary tuberculosis is associated with high nitric oxide plasma level.

El Daker S, Sacchi A, Tempestilli M, Carducci C, Goletti D, Vanini V, Colizzi V, Lauria FN, Martini F, Martino A - PLoS ONE (2015)

Bottom Line: We observed an expansion of MDSCs in the lung and blood of patients with active TB, which are correlated with an enhanced amount of nitric oxide in the plasma.We also found that these cells have the remarkable ability to suppress T-cell response, suggesting an important role in the modulation of the immune response against TB.Interestingly, a trend in the diminution of MDSCs was found after an efficacious anti-TB therapy, suggesting that these cells may be used as a potential biomarker for monitoring anti-TB therapy efficacy.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cellular Immunology, "Lazzaro Spallanzani" National Institute for Infectious Diseases, Rome, Italy; Unité de Biologie des Populations Lymphocytaires, Department of Immunology, Institut Pasteur, Paris, France; Department of Biology, University of Rome Tor Vergata, Rome, Italy.

ABSTRACT
Tuberculosis (TB) is still the principal cause of death caused by a single infectious agent, and the balance between the bacillus and host defense mechanisms reflects the different manifestations of the pathology. The aim of this work was to study the role of myeloid-derived suppressor cells (MDSCs) during active pulmonary tuberculosis at the site of infection. We observed an expansion of MDSCs in the lung and blood of patients with active TB, which are correlated with an enhanced amount of nitric oxide in the plasma. We also found that these cells have the remarkable ability to suppress T-cell response, suggesting an important role in the modulation of the immune response against TB. Interestingly, a trend in the diminution of MDSCs was found after an efficacious anti-TB therapy, suggesting that these cells may be used as a potential biomarker for monitoring anti-TB therapy efficacy.

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Successful TB treatment reduces the concentration of MDSCs in peripheral blood cells.(A) Analysis of MDSC frequency in patients with active TB (N.30) and cured TB (N.10). (B) MDSC frequency in cured TB at the end of therapy (N.5) and 1–3 years after the end of therapy (past TB, N.5). Results are expressed as the median ± IQR. *P<0.05.
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pone.0123772.g004: Successful TB treatment reduces the concentration of MDSCs in peripheral blood cells.(A) Analysis of MDSC frequency in patients with active TB (N.30) and cured TB (N.10). (B) MDSC frequency in cured TB at the end of therapy (N.5) and 1–3 years after the end of therapy (past TB, N.5). Results are expressed as the median ± IQR. *P<0.05.

Mentions: We next assessed the effect of successful anti-TB therapy on the frequency of MDSCs. Therefore, we analyzed the proportion of CD14-MDSCs in PBMCs from patients with active TB, and patients after successful therapy. We found that in cured TB patients, the frequency of CD14-MDSCs was lower compared to active TB (Fig 4A). Furthermore, an almost significant decrease of MDSC frequency was found in patients 1–3 years after therapy compared to those at the end of therapy (Fig 4B) (p = 0.055). These data suggest that successful therapy associates with a decrease of MDSC frequency.


Granulocytic myeloid derived suppressor cells expansion during active pulmonary tuberculosis is associated with high nitric oxide plasma level.

El Daker S, Sacchi A, Tempestilli M, Carducci C, Goletti D, Vanini V, Colizzi V, Lauria FN, Martini F, Martino A - PLoS ONE (2015)

Successful TB treatment reduces the concentration of MDSCs in peripheral blood cells.(A) Analysis of MDSC frequency in patients with active TB (N.30) and cured TB (N.10). (B) MDSC frequency in cured TB at the end of therapy (N.5) and 1–3 years after the end of therapy (past TB, N.5). Results are expressed as the median ± IQR. *P<0.05.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400140&req=5

pone.0123772.g004: Successful TB treatment reduces the concentration of MDSCs in peripheral blood cells.(A) Analysis of MDSC frequency in patients with active TB (N.30) and cured TB (N.10). (B) MDSC frequency in cured TB at the end of therapy (N.5) and 1–3 years after the end of therapy (past TB, N.5). Results are expressed as the median ± IQR. *P<0.05.
Mentions: We next assessed the effect of successful anti-TB therapy on the frequency of MDSCs. Therefore, we analyzed the proportion of CD14-MDSCs in PBMCs from patients with active TB, and patients after successful therapy. We found that in cured TB patients, the frequency of CD14-MDSCs was lower compared to active TB (Fig 4A). Furthermore, an almost significant decrease of MDSC frequency was found in patients 1–3 years after therapy compared to those at the end of therapy (Fig 4B) (p = 0.055). These data suggest that successful therapy associates with a decrease of MDSC frequency.

Bottom Line: We observed an expansion of MDSCs in the lung and blood of patients with active TB, which are correlated with an enhanced amount of nitric oxide in the plasma.We also found that these cells have the remarkable ability to suppress T-cell response, suggesting an important role in the modulation of the immune response against TB.Interestingly, a trend in the diminution of MDSCs was found after an efficacious anti-TB therapy, suggesting that these cells may be used as a potential biomarker for monitoring anti-TB therapy efficacy.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cellular Immunology, "Lazzaro Spallanzani" National Institute for Infectious Diseases, Rome, Italy; Unité de Biologie des Populations Lymphocytaires, Department of Immunology, Institut Pasteur, Paris, France; Department of Biology, University of Rome Tor Vergata, Rome, Italy.

ABSTRACT
Tuberculosis (TB) is still the principal cause of death caused by a single infectious agent, and the balance between the bacillus and host defense mechanisms reflects the different manifestations of the pathology. The aim of this work was to study the role of myeloid-derived suppressor cells (MDSCs) during active pulmonary tuberculosis at the site of infection. We observed an expansion of MDSCs in the lung and blood of patients with active TB, which are correlated with an enhanced amount of nitric oxide in the plasma. We also found that these cells have the remarkable ability to suppress T-cell response, suggesting an important role in the modulation of the immune response against TB. Interestingly, a trend in the diminution of MDSCs was found after an efficacious anti-TB therapy, suggesting that these cells may be used as a potential biomarker for monitoring anti-TB therapy efficacy.

Show MeSH
Related in: MedlinePlus