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Consequences of lower food intake on the digestive enzymes activities, the energy reserves and the reproductive outcome in Gammarus fossarum.

Charron L, Geffard O, Chaumot A, Coulaud R, Jaffal A, Gaillet V, Dedourge-Geffard O, Geffard A - PLoS ONE (2015)

Bottom Line: Energy reserves decreased with food starvation in females, and remained stable in males.The number of embryos per female decreased with food starvation.These results underline the ecological relevance of digestive markers, as they make it possible to anticipate upcoming consequences on reproduction in females, a key biological variable for population dynamics.

View Article: PubMed Central - PubMed

Affiliation: Université Reims Champagne Ardenne, Unité Mixte de Recherche-Ineris (UMR-I02) Stress Environnementaux et Biosurveillance des milieux aquatiques, Unité de Formation et de Recherche Sciences Exactes et Naturelles, Moulin de la Housse, Reims Cedex 2, France.

ABSTRACT
Digestive enzyme activity is often used as a sensitive response to environmental pollution. However, only little is known about the negative effects of stress on digestive capacities and their consequences on energy reserves and reproduction, although these parameters are important for the maintenance of populations. To highlight if changes in biochemical responses (digestive enzymes and reserves) led to impairments at an individual level (fertility), Gammarus fossarum were submitted to a lower food intake throughout a complete female reproductive cycle (i.e. from ovogenesis to offspring production). For both males and females, amylase activity was inhibited by the diet stress, whereas trypsin activity was not influenced. These results underline similar sensitivity of males and females concerning their digestive capacity. Energy reserves decreased with food starvation in females, and remained stable in males. The number of embryos per female decreased with food starvation. Lower digestive activity in males and females therefore appears as an early response. These results underline the ecological relevance of digestive markers, as they make it possible to anticipate upcoming consequences on reproduction in females, a key biological variable for population dynamics.

No MeSH data available.


Related in: MedlinePlus

Mean embryo numbers in Gammarus fossarum females exposed to 3 levels of diet starvation (control: fed 7 days a week; 2/7: fed 2 days a week; 1/7: fed 1 day a week) after 43 days (means ± SD, n = 6).Bars with the same letter were not significantly different (p < 0.05).
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pone.0125154.g004: Mean embryo numbers in Gammarus fossarum females exposed to 3 levels of diet starvation (control: fed 7 days a week; 2/7: fed 2 days a week; 1/7: fed 1 day a week) after 43 days (means ± SD, n = 6).Bars with the same letter were not significantly different (p < 0.05).

Mentions: The number of embryos per female (i.e. fertility) was counted after 43 days of food stress (Fig 4). Only embryos from females at the AB or C1 stage were investigated. Starvation resulted in an embryo loss of 19% in 2/7 females (p = 0.025) and 39% in 1/7 females (p = 0.0039), compared to the controls fed 7/7 days.


Consequences of lower food intake on the digestive enzymes activities, the energy reserves and the reproductive outcome in Gammarus fossarum.

Charron L, Geffard O, Chaumot A, Coulaud R, Jaffal A, Gaillet V, Dedourge-Geffard O, Geffard A - PLoS ONE (2015)

Mean embryo numbers in Gammarus fossarum females exposed to 3 levels of diet starvation (control: fed 7 days a week; 2/7: fed 2 days a week; 1/7: fed 1 day a week) after 43 days (means ± SD, n = 6).Bars with the same letter were not significantly different (p < 0.05).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400123&req=5

pone.0125154.g004: Mean embryo numbers in Gammarus fossarum females exposed to 3 levels of diet starvation (control: fed 7 days a week; 2/7: fed 2 days a week; 1/7: fed 1 day a week) after 43 days (means ± SD, n = 6).Bars with the same letter were not significantly different (p < 0.05).
Mentions: The number of embryos per female (i.e. fertility) was counted after 43 days of food stress (Fig 4). Only embryos from females at the AB or C1 stage were investigated. Starvation resulted in an embryo loss of 19% in 2/7 females (p = 0.025) and 39% in 1/7 females (p = 0.0039), compared to the controls fed 7/7 days.

Bottom Line: Energy reserves decreased with food starvation in females, and remained stable in males.The number of embryos per female decreased with food starvation.These results underline the ecological relevance of digestive markers, as they make it possible to anticipate upcoming consequences on reproduction in females, a key biological variable for population dynamics.

View Article: PubMed Central - PubMed

Affiliation: Université Reims Champagne Ardenne, Unité Mixte de Recherche-Ineris (UMR-I02) Stress Environnementaux et Biosurveillance des milieux aquatiques, Unité de Formation et de Recherche Sciences Exactes et Naturelles, Moulin de la Housse, Reims Cedex 2, France.

ABSTRACT
Digestive enzyme activity is often used as a sensitive response to environmental pollution. However, only little is known about the negative effects of stress on digestive capacities and their consequences on energy reserves and reproduction, although these parameters are important for the maintenance of populations. To highlight if changes in biochemical responses (digestive enzymes and reserves) led to impairments at an individual level (fertility), Gammarus fossarum were submitted to a lower food intake throughout a complete female reproductive cycle (i.e. from ovogenesis to offspring production). For both males and females, amylase activity was inhibited by the diet stress, whereas trypsin activity was not influenced. These results underline similar sensitivity of males and females concerning their digestive capacity. Energy reserves decreased with food starvation in females, and remained stable in males. The number of embryos per female decreased with food starvation. Lower digestive activity in males and females therefore appears as an early response. These results underline the ecological relevance of digestive markers, as they make it possible to anticipate upcoming consequences on reproduction in females, a key biological variable for population dynamics.

No MeSH data available.


Related in: MedlinePlus