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Consequences of lower food intake on the digestive enzymes activities, the energy reserves and the reproductive outcome in Gammarus fossarum.

Charron L, Geffard O, Chaumot A, Coulaud R, Jaffal A, Gaillet V, Dedourge-Geffard O, Geffard A - PLoS ONE (2015)

Bottom Line: Energy reserves decreased with food starvation in females, and remained stable in males.The number of embryos per female decreased with food starvation.These results underline the ecological relevance of digestive markers, as they make it possible to anticipate upcoming consequences on reproduction in females, a key biological variable for population dynamics.

View Article: PubMed Central - PubMed

Affiliation: Université Reims Champagne Ardenne, Unité Mixte de Recherche-Ineris (UMR-I02) Stress Environnementaux et Biosurveillance des milieux aquatiques, Unité de Formation et de Recherche Sciences Exactes et Naturelles, Moulin de la Housse, Reims Cedex 2, France.

ABSTRACT
Digestive enzyme activity is often used as a sensitive response to environmental pollution. However, only little is known about the negative effects of stress on digestive capacities and their consequences on energy reserves and reproduction, although these parameters are important for the maintenance of populations. To highlight if changes in biochemical responses (digestive enzymes and reserves) led to impairments at an individual level (fertility), Gammarus fossarum were submitted to a lower food intake throughout a complete female reproductive cycle (i.e. from ovogenesis to offspring production). For both males and females, amylase activity was inhibited by the diet stress, whereas trypsin activity was not influenced. These results underline similar sensitivity of males and females concerning their digestive capacity. Energy reserves decreased with food starvation in females, and remained stable in males. The number of embryos per female decreased with food starvation. Lower digestive activity in males and females therefore appears as an early response. These results underline the ecological relevance of digestive markers, as they make it possible to anticipate upcoming consequences on reproduction in females, a key biological variable for population dynamics.

No MeSH data available.


Related in: MedlinePlus

Available energy (mJ/mg wet weight) in Gammarus fossarum exposed to 3 levels of food starvation (controls: fed 7 days a week; 2/7: fed 2 days a week; 1/7: fed 1 day a week) after 11 and 43 days (means ± SD, n = 6).For each date and gender, bars with the same letter were not significantly different (p < 0.05). The hash (#) symbol points significant changes between the two sampling times in each diet condition (p < 0.05).
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pone.0125154.g003: Available energy (mJ/mg wet weight) in Gammarus fossarum exposed to 3 levels of food starvation (controls: fed 7 days a week; 2/7: fed 2 days a week; 1/7: fed 1 day a week) after 11 and 43 days (means ± SD, n = 6).For each date and gender, bars with the same letter were not significantly different (p < 0.05). The hash (#) symbol points significant changes between the two sampling times in each diet condition (p < 0.05).

Mentions: In females, food starvation significantly reduced available energy for the both cycle (p = 0.0005 for day 11 and p = 0.0008 for day 43) (Fig 3). From the first reproductive cycle (after 11 days), available energy values in 1/7 and 2/7 females were about one third lower than in the controls (p = 0.0039 between 1/7 and 7/7 and p = 0.0039 between 2/7 and 7/7). Over the second reproductive cycle (after 43 days), 1/7 and 2/7 females exhibited average energy values 20% and 28% lower than available energy in control females (p = 0.0039 between 1/7 and 7/7 and p = 0.039 between 2/7 and 7/7).


Consequences of lower food intake on the digestive enzymes activities, the energy reserves and the reproductive outcome in Gammarus fossarum.

Charron L, Geffard O, Chaumot A, Coulaud R, Jaffal A, Gaillet V, Dedourge-Geffard O, Geffard A - PLoS ONE (2015)

Available energy (mJ/mg wet weight) in Gammarus fossarum exposed to 3 levels of food starvation (controls: fed 7 days a week; 2/7: fed 2 days a week; 1/7: fed 1 day a week) after 11 and 43 days (means ± SD, n = 6).For each date and gender, bars with the same letter were not significantly different (p < 0.05). The hash (#) symbol points significant changes between the two sampling times in each diet condition (p < 0.05).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400123&req=5

pone.0125154.g003: Available energy (mJ/mg wet weight) in Gammarus fossarum exposed to 3 levels of food starvation (controls: fed 7 days a week; 2/7: fed 2 days a week; 1/7: fed 1 day a week) after 11 and 43 days (means ± SD, n = 6).For each date and gender, bars with the same letter were not significantly different (p < 0.05). The hash (#) symbol points significant changes between the two sampling times in each diet condition (p < 0.05).
Mentions: In females, food starvation significantly reduced available energy for the both cycle (p = 0.0005 for day 11 and p = 0.0008 for day 43) (Fig 3). From the first reproductive cycle (after 11 days), available energy values in 1/7 and 2/7 females were about one third lower than in the controls (p = 0.0039 between 1/7 and 7/7 and p = 0.0039 between 2/7 and 7/7). Over the second reproductive cycle (after 43 days), 1/7 and 2/7 females exhibited average energy values 20% and 28% lower than available energy in control females (p = 0.0039 between 1/7 and 7/7 and p = 0.039 between 2/7 and 7/7).

Bottom Line: Energy reserves decreased with food starvation in females, and remained stable in males.The number of embryos per female decreased with food starvation.These results underline the ecological relevance of digestive markers, as they make it possible to anticipate upcoming consequences on reproduction in females, a key biological variable for population dynamics.

View Article: PubMed Central - PubMed

Affiliation: Université Reims Champagne Ardenne, Unité Mixte de Recherche-Ineris (UMR-I02) Stress Environnementaux et Biosurveillance des milieux aquatiques, Unité de Formation et de Recherche Sciences Exactes et Naturelles, Moulin de la Housse, Reims Cedex 2, France.

ABSTRACT
Digestive enzyme activity is often used as a sensitive response to environmental pollution. However, only little is known about the negative effects of stress on digestive capacities and their consequences on energy reserves and reproduction, although these parameters are important for the maintenance of populations. To highlight if changes in biochemical responses (digestive enzymes and reserves) led to impairments at an individual level (fertility), Gammarus fossarum were submitted to a lower food intake throughout a complete female reproductive cycle (i.e. from ovogenesis to offspring production). For both males and females, amylase activity was inhibited by the diet stress, whereas trypsin activity was not influenced. These results underline similar sensitivity of males and females concerning their digestive capacity. Energy reserves decreased with food starvation in females, and remained stable in males. The number of embryos per female decreased with food starvation. Lower digestive activity in males and females therefore appears as an early response. These results underline the ecological relevance of digestive markers, as they make it possible to anticipate upcoming consequences on reproduction in females, a key biological variable for population dynamics.

No MeSH data available.


Related in: MedlinePlus