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Herbal supplement extends life span under some environmental conditions and boosts stress resistance.

Villeponteau B, Matsagas K, Nobles AC, Rizza C, Horwitz M, Benford G, Mockett RJ - PLoS ONE (2015)

Bottom Line: In contrast, at an independent test site where stress was minimized, the flies exhibited much longer mean life spans, but the survival curves became highly rectangular and the effects of SC100 on both mean and maximum life spans declined greatly or were abolished.The data indicate that SC100 is a novel herbal mix with striking effects on enhancing Drosophila stress resistance and life span in some environments, while minimizing mid to late life mortality rates.They also show that the environment and other factors can have transformative effects on both the length and distribution of survivorship, and on the ability of SC100 to extend the life span.

View Article: PubMed Central - PubMed

Affiliation: Genescient Inc., Fountain Valley, California, United States of America.

ABSTRACT
Genetic studies indicate that aging is modulated by a great number of genetic pathways. We have used Drosophila longevity and stress assays to test a multipath intervention strategy. To carry out this strategy, we supplemented the flies with herbal extracts (SC100) that are predicted to modulate the expression of many genes involved in aging and stress resistance, such as mTOR, NOS, NF-KappaB, and VEGF. When flies were housed in large cages with SC100 added, daily mortality rates of both male and female flies were greatly diminished in mid to late life. Surprisingly, SC100 also stabilized midlife mortality rate increases so as to extend the maximum life span substantially beyond the limits previously reported for D. melanogaster. Under these conditions, SC100 also promoted robust resistance to partial starvation stress and to heat stress. Fertility was the same initially in both treated and control flies, but it became significantly higher in treated flies at older ages as the fertility of control flies declined. Mean and maximum life spans of flies in vials at the same test site were also extended by SC100, but the life spans were short in absolute terms. In contrast, at an independent test site where stress was minimized, the flies exhibited much longer mean life spans, but the survival curves became highly rectangular and the effects of SC100 on both mean and maximum life spans declined greatly or were abolished. The data indicate that SC100 is a novel herbal mix with striking effects on enhancing Drosophila stress resistance and life span in some environments, while minimizing mid to late life mortality rates. They also show that the environment and other factors can have transformative effects on both the length and distribution of survivorship, and on the ability of SC100 to extend the life span.

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Life span of female and male B4 Drosophila in single sex vials with or without SC treatment: AL site.Flies were separated by sex 1 d after eclosion, housed in groups of 25/vial at 25°C on a 12 h light: 12 h dark cycle, and supplemented with SC100 at doses of 0× (solid green triangles), 1/3× (solid blue squares), 1× (open black squares), and 3× (solid red squares) beginning 2 d after collection. Results are presented separately for females (A,B) and males (C,D) on banana medium (BM; A,C) and cornmeal (CM; B,D). For each medium and dosage, n = 3–4 vials (72–98 surviving flies) for females and 4 vials (89–100 survivors) for males. Results presented in Figs 4–6 were obtained concurrently.
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pone.0119068.g004: Life span of female and male B4 Drosophila in single sex vials with or without SC treatment: AL site.Flies were separated by sex 1 d after eclosion, housed in groups of 25/vial at 25°C on a 12 h light: 12 h dark cycle, and supplemented with SC100 at doses of 0× (solid green triangles), 1/3× (solid blue squares), 1× (open black squares), and 3× (solid red squares) beginning 2 d after collection. Results are presented separately for females (A,B) and males (C,D) on banana medium (BM; A,C) and cornmeal (CM; B,D). For each medium and dosage, n = 3–4 vials (72–98 surviving flies) for females and 4 vials (89–100 survivors) for males. Results presented in Figs 4–6 were obtained concurrently.

Mentions: To establish the consistency of effects of SC100 under different strain, food, and housing conditions, the supplement was tested by a separate group of investigators at an independent test site in Alabama (AL site) using low stress (single sex and 12 h on/12 h off lighting) vial conditions. SC100 was administered simultaneously to both female and male flies of three different genotypes provided with two different media, at three doses spanning a 9-fold range (1/3×, 1× and 3×). Flies housed in vials at the AL site exhibited highly rectangular survival curves, with no hint of a mortality plateau at advanced ages (Figs 4–6). Despite the 6 fold higher population density of flies in AL, the mean life spans of untreated (0×) flies were substantially longer for all groups in AL than CA, by factors of 2.7 for B females (68.7 vs. 25.2 d) and 2.6 for B males (68.1 vs. 25.9 d) on the same BM medium used in vials in CA. On the other hand, the maximum life span (90–100% mortality) was substantially shorter for supplemented flies in vials in AL than in cages in CA.


Herbal supplement extends life span under some environmental conditions and boosts stress resistance.

Villeponteau B, Matsagas K, Nobles AC, Rizza C, Horwitz M, Benford G, Mockett RJ - PLoS ONE (2015)

Life span of female and male B4 Drosophila in single sex vials with or without SC treatment: AL site.Flies were separated by sex 1 d after eclosion, housed in groups of 25/vial at 25°C on a 12 h light: 12 h dark cycle, and supplemented with SC100 at doses of 0× (solid green triangles), 1/3× (solid blue squares), 1× (open black squares), and 3× (solid red squares) beginning 2 d after collection. Results are presented separately for females (A,B) and males (C,D) on banana medium (BM; A,C) and cornmeal (CM; B,D). For each medium and dosage, n = 3–4 vials (72–98 surviving flies) for females and 4 vials (89–100 survivors) for males. Results presented in Figs 4–6 were obtained concurrently.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400117&req=5

pone.0119068.g004: Life span of female and male B4 Drosophila in single sex vials with or without SC treatment: AL site.Flies were separated by sex 1 d after eclosion, housed in groups of 25/vial at 25°C on a 12 h light: 12 h dark cycle, and supplemented with SC100 at doses of 0× (solid green triangles), 1/3× (solid blue squares), 1× (open black squares), and 3× (solid red squares) beginning 2 d after collection. Results are presented separately for females (A,B) and males (C,D) on banana medium (BM; A,C) and cornmeal (CM; B,D). For each medium and dosage, n = 3–4 vials (72–98 surviving flies) for females and 4 vials (89–100 survivors) for males. Results presented in Figs 4–6 were obtained concurrently.
Mentions: To establish the consistency of effects of SC100 under different strain, food, and housing conditions, the supplement was tested by a separate group of investigators at an independent test site in Alabama (AL site) using low stress (single sex and 12 h on/12 h off lighting) vial conditions. SC100 was administered simultaneously to both female and male flies of three different genotypes provided with two different media, at three doses spanning a 9-fold range (1/3×, 1× and 3×). Flies housed in vials at the AL site exhibited highly rectangular survival curves, with no hint of a mortality plateau at advanced ages (Figs 4–6). Despite the 6 fold higher population density of flies in AL, the mean life spans of untreated (0×) flies were substantially longer for all groups in AL than CA, by factors of 2.7 for B females (68.7 vs. 25.2 d) and 2.6 for B males (68.1 vs. 25.9 d) on the same BM medium used in vials in CA. On the other hand, the maximum life span (90–100% mortality) was substantially shorter for supplemented flies in vials in AL than in cages in CA.

Bottom Line: In contrast, at an independent test site where stress was minimized, the flies exhibited much longer mean life spans, but the survival curves became highly rectangular and the effects of SC100 on both mean and maximum life spans declined greatly or were abolished.The data indicate that SC100 is a novel herbal mix with striking effects on enhancing Drosophila stress resistance and life span in some environments, while minimizing mid to late life mortality rates.They also show that the environment and other factors can have transformative effects on both the length and distribution of survivorship, and on the ability of SC100 to extend the life span.

View Article: PubMed Central - PubMed

Affiliation: Genescient Inc., Fountain Valley, California, United States of America.

ABSTRACT
Genetic studies indicate that aging is modulated by a great number of genetic pathways. We have used Drosophila longevity and stress assays to test a multipath intervention strategy. To carry out this strategy, we supplemented the flies with herbal extracts (SC100) that are predicted to modulate the expression of many genes involved in aging and stress resistance, such as mTOR, NOS, NF-KappaB, and VEGF. When flies were housed in large cages with SC100 added, daily mortality rates of both male and female flies were greatly diminished in mid to late life. Surprisingly, SC100 also stabilized midlife mortality rate increases so as to extend the maximum life span substantially beyond the limits previously reported for D. melanogaster. Under these conditions, SC100 also promoted robust resistance to partial starvation stress and to heat stress. Fertility was the same initially in both treated and control flies, but it became significantly higher in treated flies at older ages as the fertility of control flies declined. Mean and maximum life spans of flies in vials at the same test site were also extended by SC100, but the life spans were short in absolute terms. In contrast, at an independent test site where stress was minimized, the flies exhibited much longer mean life spans, but the survival curves became highly rectangular and the effects of SC100 on both mean and maximum life spans declined greatly or were abolished. The data indicate that SC100 is a novel herbal mix with striking effects on enhancing Drosophila stress resistance and life span in some environments, while minimizing mid to late life mortality rates. They also show that the environment and other factors can have transformative effects on both the length and distribution of survivorship, and on the ability of SC100 to extend the life span.

Show MeSH
Related in: MedlinePlus