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Herbal supplement extends life span under some environmental conditions and boosts stress resistance.

Villeponteau B, Matsagas K, Nobles AC, Rizza C, Horwitz M, Benford G, Mockett RJ - PLoS ONE (2015)

Bottom Line: In contrast, at an independent test site where stress was minimized, the flies exhibited much longer mean life spans, but the survival curves became highly rectangular and the effects of SC100 on both mean and maximum life spans declined greatly or were abolished.The data indicate that SC100 is a novel herbal mix with striking effects on enhancing Drosophila stress resistance and life span in some environments, while minimizing mid to late life mortality rates.They also show that the environment and other factors can have transformative effects on both the length and distribution of survivorship, and on the ability of SC100 to extend the life span.

View Article: PubMed Central - PubMed

Affiliation: Genescient Inc., Fountain Valley, California, United States of America.

ABSTRACT
Genetic studies indicate that aging is modulated by a great number of genetic pathways. We have used Drosophila longevity and stress assays to test a multipath intervention strategy. To carry out this strategy, we supplemented the flies with herbal extracts (SC100) that are predicted to modulate the expression of many genes involved in aging and stress resistance, such as mTOR, NOS, NF-KappaB, and VEGF. When flies were housed in large cages with SC100 added, daily mortality rates of both male and female flies were greatly diminished in mid to late life. Surprisingly, SC100 also stabilized midlife mortality rate increases so as to extend the maximum life span substantially beyond the limits previously reported for D. melanogaster. Under these conditions, SC100 also promoted robust resistance to partial starvation stress and to heat stress. Fertility was the same initially in both treated and control flies, but it became significantly higher in treated flies at older ages as the fertility of control flies declined. Mean and maximum life spans of flies in vials at the same test site were also extended by SC100, but the life spans were short in absolute terms. In contrast, at an independent test site where stress was minimized, the flies exhibited much longer mean life spans, but the survival curves became highly rectangular and the effects of SC100 on both mean and maximum life spans declined greatly or were abolished. The data indicate that SC100 is a novel herbal mix with striking effects on enhancing Drosophila stress resistance and life span in some environments, while minimizing mid to late life mortality rates. They also show that the environment and other factors can have transformative effects on both the length and distribution of survivorship, and on the ability of SC100 to extend the life span.

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Related in: MedlinePlus

Life span of male and female Drosophila in single sex or combined sex vials with or without SC100 treatment: CA site.3A (Females) and 3B (Males) show the survivorship curves for female and male B4 flies, respectively, where 4 flies of the same sex or 2 flies of each sex were housed at 25°C in each test vial, with 100 flies for each sex in the control or SC100-treated samples. In (A), the data for females are shown: Control females were housed in separate vials from males (solid green circles) versus housing of females with males in combined vials (solid green triangles); SC100 treated female flies are housed separate from males (open blue circles) or combined with males (open blue triangles). In (B), the data for males are shown: Control males were housed in separate vials from females (solid green circles) versus housing of males with females in combined vials (solid green triangles); SC100 treated male flies were housed separate from females (open blue circles) or combined with females (open blue triangles).
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pone.0119068.g003: Life span of male and female Drosophila in single sex or combined sex vials with or without SC100 treatment: CA site.3A (Females) and 3B (Males) show the survivorship curves for female and male B4 flies, respectively, where 4 flies of the same sex or 2 flies of each sex were housed at 25°C in each test vial, with 100 flies for each sex in the control or SC100-treated samples. In (A), the data for females are shown: Control females were housed in separate vials from males (solid green circles) versus housing of females with males in combined vials (solid green triangles); SC100 treated female flies are housed separate from males (open blue circles) or combined with males (open blue triangles). In (B), the data for males are shown: Control males were housed in separate vials from females (solid green circles) versus housing of males with females in combined vials (solid green triangles); SC100 treated male flies were housed separate from females (open blue circles) or combined with females (open blue triangles).

Mentions: The 500 fly cage longevity screening assay described above is not used as commonly as small test vial experiments where the Drosophila are tightly confined as to space and unable to fly. In most test vial fly assays, male and female flies are separated into different vials, as it is well known that sexual activity shortens fly life span. Since the test tube-like vial environment is very different from the cage environment, we tested SC100 on flies in vials combined with or separated from the opposite sex. Fig 3A and 3B show the survivorship curves for females and males respectively, where 4 flies of the same sex or combined sexes were housed in each test vial with 100 flies for each sex in each control and 100 flies for each sex in each of the SC100-treated samples.


Herbal supplement extends life span under some environmental conditions and boosts stress resistance.

Villeponteau B, Matsagas K, Nobles AC, Rizza C, Horwitz M, Benford G, Mockett RJ - PLoS ONE (2015)

Life span of male and female Drosophila in single sex or combined sex vials with or without SC100 treatment: CA site.3A (Females) and 3B (Males) show the survivorship curves for female and male B4 flies, respectively, where 4 flies of the same sex or 2 flies of each sex were housed at 25°C in each test vial, with 100 flies for each sex in the control or SC100-treated samples. In (A), the data for females are shown: Control females were housed in separate vials from males (solid green circles) versus housing of females with males in combined vials (solid green triangles); SC100 treated female flies are housed separate from males (open blue circles) or combined with males (open blue triangles). In (B), the data for males are shown: Control males were housed in separate vials from females (solid green circles) versus housing of males with females in combined vials (solid green triangles); SC100 treated male flies were housed separate from females (open blue circles) or combined with females (open blue triangles).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400117&req=5

pone.0119068.g003: Life span of male and female Drosophila in single sex or combined sex vials with or without SC100 treatment: CA site.3A (Females) and 3B (Males) show the survivorship curves for female and male B4 flies, respectively, where 4 flies of the same sex or 2 flies of each sex were housed at 25°C in each test vial, with 100 flies for each sex in the control or SC100-treated samples. In (A), the data for females are shown: Control females were housed in separate vials from males (solid green circles) versus housing of females with males in combined vials (solid green triangles); SC100 treated female flies are housed separate from males (open blue circles) or combined with males (open blue triangles). In (B), the data for males are shown: Control males were housed in separate vials from females (solid green circles) versus housing of males with females in combined vials (solid green triangles); SC100 treated male flies were housed separate from females (open blue circles) or combined with females (open blue triangles).
Mentions: The 500 fly cage longevity screening assay described above is not used as commonly as small test vial experiments where the Drosophila are tightly confined as to space and unable to fly. In most test vial fly assays, male and female flies are separated into different vials, as it is well known that sexual activity shortens fly life span. Since the test tube-like vial environment is very different from the cage environment, we tested SC100 on flies in vials combined with or separated from the opposite sex. Fig 3A and 3B show the survivorship curves for females and males respectively, where 4 flies of the same sex or combined sexes were housed in each test vial with 100 flies for each sex in each control and 100 flies for each sex in each of the SC100-treated samples.

Bottom Line: In contrast, at an independent test site where stress was minimized, the flies exhibited much longer mean life spans, but the survival curves became highly rectangular and the effects of SC100 on both mean and maximum life spans declined greatly or were abolished.The data indicate that SC100 is a novel herbal mix with striking effects on enhancing Drosophila stress resistance and life span in some environments, while minimizing mid to late life mortality rates.They also show that the environment and other factors can have transformative effects on both the length and distribution of survivorship, and on the ability of SC100 to extend the life span.

View Article: PubMed Central - PubMed

Affiliation: Genescient Inc., Fountain Valley, California, United States of America.

ABSTRACT
Genetic studies indicate that aging is modulated by a great number of genetic pathways. We have used Drosophila longevity and stress assays to test a multipath intervention strategy. To carry out this strategy, we supplemented the flies with herbal extracts (SC100) that are predicted to modulate the expression of many genes involved in aging and stress resistance, such as mTOR, NOS, NF-KappaB, and VEGF. When flies were housed in large cages with SC100 added, daily mortality rates of both male and female flies were greatly diminished in mid to late life. Surprisingly, SC100 also stabilized midlife mortality rate increases so as to extend the maximum life span substantially beyond the limits previously reported for D. melanogaster. Under these conditions, SC100 also promoted robust resistance to partial starvation stress and to heat stress. Fertility was the same initially in both treated and control flies, but it became significantly higher in treated flies at older ages as the fertility of control flies declined. Mean and maximum life spans of flies in vials at the same test site were also extended by SC100, but the life spans were short in absolute terms. In contrast, at an independent test site where stress was minimized, the flies exhibited much longer mean life spans, but the survival curves became highly rectangular and the effects of SC100 on both mean and maximum life spans declined greatly or were abolished. The data indicate that SC100 is a novel herbal mix with striking effects on enhancing Drosophila stress resistance and life span in some environments, while minimizing mid to late life mortality rates. They also show that the environment and other factors can have transformative effects on both the length and distribution of survivorship, and on the ability of SC100 to extend the life span.

Show MeSH
Related in: MedlinePlus