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Herbal supplement extends life span under some environmental conditions and boosts stress resistance.

Villeponteau B, Matsagas K, Nobles AC, Rizza C, Horwitz M, Benford G, Mockett RJ - PLoS ONE (2015)

Bottom Line: In contrast, at an independent test site where stress was minimized, the flies exhibited much longer mean life spans, but the survival curves became highly rectangular and the effects of SC100 on both mean and maximum life spans declined greatly or were abolished.The data indicate that SC100 is a novel herbal mix with striking effects on enhancing Drosophila stress resistance and life span in some environments, while minimizing mid to late life mortality rates.They also show that the environment and other factors can have transformative effects on both the length and distribution of survivorship, and on the ability of SC100 to extend the life span.

View Article: PubMed Central - PubMed

Affiliation: Genescient Inc., Fountain Valley, California, United States of America.

ABSTRACT
Genetic studies indicate that aging is modulated by a great number of genetic pathways. We have used Drosophila longevity and stress assays to test a multipath intervention strategy. To carry out this strategy, we supplemented the flies with herbal extracts (SC100) that are predicted to modulate the expression of many genes involved in aging and stress resistance, such as mTOR, NOS, NF-KappaB, and VEGF. When flies were housed in large cages with SC100 added, daily mortality rates of both male and female flies were greatly diminished in mid to late life. Surprisingly, SC100 also stabilized midlife mortality rate increases so as to extend the maximum life span substantially beyond the limits previously reported for D. melanogaster. Under these conditions, SC100 also promoted robust resistance to partial starvation stress and to heat stress. Fertility was the same initially in both treated and control flies, but it became significantly higher in treated flies at older ages as the fertility of control flies declined. Mean and maximum life spans of flies in vials at the same test site were also extended by SC100, but the life spans were short in absolute terms. In contrast, at an independent test site where stress was minimized, the flies exhibited much longer mean life spans, but the survival curves became highly rectangular and the effects of SC100 on both mean and maximum life spans declined greatly or were abolished. The data indicate that SC100 is a novel herbal mix with striking effects on enhancing Drosophila stress resistance and life span in some environments, while minimizing mid to late life mortality rates. They also show that the environment and other factors can have transformative effects on both the length and distribution of survivorship, and on the ability of SC100 to extend the life span.

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Related in: MedlinePlus

Male and female mortality rate changes in cages with or without SC100 treatment.(A) These data are from the same longevity assays shown in Fig 1, wherein nine independent cages were used, with each cage having 250 males and 250 female B4 flies. To correlate mortality rates with the longevity assays, the male longevity data in the 3 sets of cages of Fig 1D, 1E, and 1F were pooled and composite average values were taken on each day for: C) Control untreated male flies (green triangles), T) SC100 treated male flies (blue squares), and CT) Control/Treated flies that were switched to SC100 treatment on day 36 of the assay (red diamonds). (B) and (C) show composite daily mortality rates for male flies (B) and female flies (C), which were determined by averaging the daily mortality rates from the three sets of cages for each treatment or control.
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pone.0119068.g002: Male and female mortality rate changes in cages with or without SC100 treatment.(A) These data are from the same longevity assays shown in Fig 1, wherein nine independent cages were used, with each cage having 250 males and 250 female B4 flies. To correlate mortality rates with the longevity assays, the male longevity data in the 3 sets of cages of Fig 1D, 1E, and 1F were pooled and composite average values were taken on each day for: C) Control untreated male flies (green triangles), T) SC100 treated male flies (blue squares), and CT) Control/Treated flies that were switched to SC100 treatment on day 36 of the assay (red diamonds). (B) and (C) show composite daily mortality rates for male flies (B) and female flies (C), which were determined by averaging the daily mortality rates from the three sets of cages for each treatment or control.

Mentions: Another way to look at these longevity data for flies in cages is to look at the daily mortality rate (Fig 2). The control flies (green triangles) had an increase in their daily mortality rate at around 28 d and their mortality rate gradually increased until 54–58 d, when the daily mortality rate spiked as the last flies died. In both the lifetime SC100 treated flies (T, open blue squares) and the mid-life SC100 treated flies (CT, red diamonds), the mortality rate did not increase substantially until around 120 d, ending in a spike at 120–134 d. The midlife SC100 treated flies actually decreased their mortality rate several days after SC100 treatment commenced at 36 d and continued with a low stable mortality rate until the mortality spike at 120–134 d (Fig 2B and 2C). Thus, SC100 stabilized the midlife mortality rate in the flies and that may play a role in how SC100 extended maximum life span of a major fraction of the fly population.


Herbal supplement extends life span under some environmental conditions and boosts stress resistance.

Villeponteau B, Matsagas K, Nobles AC, Rizza C, Horwitz M, Benford G, Mockett RJ - PLoS ONE (2015)

Male and female mortality rate changes in cages with or without SC100 treatment.(A) These data are from the same longevity assays shown in Fig 1, wherein nine independent cages were used, with each cage having 250 males and 250 female B4 flies. To correlate mortality rates with the longevity assays, the male longevity data in the 3 sets of cages of Fig 1D, 1E, and 1F were pooled and composite average values were taken on each day for: C) Control untreated male flies (green triangles), T) SC100 treated male flies (blue squares), and CT) Control/Treated flies that were switched to SC100 treatment on day 36 of the assay (red diamonds). (B) and (C) show composite daily mortality rates for male flies (B) and female flies (C), which were determined by averaging the daily mortality rates from the three sets of cages for each treatment or control.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400117&req=5

pone.0119068.g002: Male and female mortality rate changes in cages with or without SC100 treatment.(A) These data are from the same longevity assays shown in Fig 1, wherein nine independent cages were used, with each cage having 250 males and 250 female B4 flies. To correlate mortality rates with the longevity assays, the male longevity data in the 3 sets of cages of Fig 1D, 1E, and 1F were pooled and composite average values were taken on each day for: C) Control untreated male flies (green triangles), T) SC100 treated male flies (blue squares), and CT) Control/Treated flies that were switched to SC100 treatment on day 36 of the assay (red diamonds). (B) and (C) show composite daily mortality rates for male flies (B) and female flies (C), which were determined by averaging the daily mortality rates from the three sets of cages for each treatment or control.
Mentions: Another way to look at these longevity data for flies in cages is to look at the daily mortality rate (Fig 2). The control flies (green triangles) had an increase in their daily mortality rate at around 28 d and their mortality rate gradually increased until 54–58 d, when the daily mortality rate spiked as the last flies died. In both the lifetime SC100 treated flies (T, open blue squares) and the mid-life SC100 treated flies (CT, red diamonds), the mortality rate did not increase substantially until around 120 d, ending in a spike at 120–134 d. The midlife SC100 treated flies actually decreased their mortality rate several days after SC100 treatment commenced at 36 d and continued with a low stable mortality rate until the mortality spike at 120–134 d (Fig 2B and 2C). Thus, SC100 stabilized the midlife mortality rate in the flies and that may play a role in how SC100 extended maximum life span of a major fraction of the fly population.

Bottom Line: In contrast, at an independent test site where stress was minimized, the flies exhibited much longer mean life spans, but the survival curves became highly rectangular and the effects of SC100 on both mean and maximum life spans declined greatly or were abolished.The data indicate that SC100 is a novel herbal mix with striking effects on enhancing Drosophila stress resistance and life span in some environments, while minimizing mid to late life mortality rates.They also show that the environment and other factors can have transformative effects on both the length and distribution of survivorship, and on the ability of SC100 to extend the life span.

View Article: PubMed Central - PubMed

Affiliation: Genescient Inc., Fountain Valley, California, United States of America.

ABSTRACT
Genetic studies indicate that aging is modulated by a great number of genetic pathways. We have used Drosophila longevity and stress assays to test a multipath intervention strategy. To carry out this strategy, we supplemented the flies with herbal extracts (SC100) that are predicted to modulate the expression of many genes involved in aging and stress resistance, such as mTOR, NOS, NF-KappaB, and VEGF. When flies were housed in large cages with SC100 added, daily mortality rates of both male and female flies were greatly diminished in mid to late life. Surprisingly, SC100 also stabilized midlife mortality rate increases so as to extend the maximum life span substantially beyond the limits previously reported for D. melanogaster. Under these conditions, SC100 also promoted robust resistance to partial starvation stress and to heat stress. Fertility was the same initially in both treated and control flies, but it became significantly higher in treated flies at older ages as the fertility of control flies declined. Mean and maximum life spans of flies in vials at the same test site were also extended by SC100, but the life spans were short in absolute terms. In contrast, at an independent test site where stress was minimized, the flies exhibited much longer mean life spans, but the survival curves became highly rectangular and the effects of SC100 on both mean and maximum life spans declined greatly or were abolished. The data indicate that SC100 is a novel herbal mix with striking effects on enhancing Drosophila stress resistance and life span in some environments, while minimizing mid to late life mortality rates. They also show that the environment and other factors can have transformative effects on both the length and distribution of survivorship, and on the ability of SC100 to extend the life span.

Show MeSH
Related in: MedlinePlus