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Herbal supplement extends life span under some environmental conditions and boosts stress resistance.

Villeponteau B, Matsagas K, Nobles AC, Rizza C, Horwitz M, Benford G, Mockett RJ - PLoS ONE (2015)

Bottom Line: In contrast, at an independent test site where stress was minimized, the flies exhibited much longer mean life spans, but the survival curves became highly rectangular and the effects of SC100 on both mean and maximum life spans declined greatly or were abolished.The data indicate that SC100 is a novel herbal mix with striking effects on enhancing Drosophila stress resistance and life span in some environments, while minimizing mid to late life mortality rates.They also show that the environment and other factors can have transformative effects on both the length and distribution of survivorship, and on the ability of SC100 to extend the life span.

View Article: PubMed Central - PubMed

Affiliation: Genescient Inc., Fountain Valley, California, United States of America.

ABSTRACT
Genetic studies indicate that aging is modulated by a great number of genetic pathways. We have used Drosophila longevity and stress assays to test a multipath intervention strategy. To carry out this strategy, we supplemented the flies with herbal extracts (SC100) that are predicted to modulate the expression of many genes involved in aging and stress resistance, such as mTOR, NOS, NF-KappaB, and VEGF. When flies were housed in large cages with SC100 added, daily mortality rates of both male and female flies were greatly diminished in mid to late life. Surprisingly, SC100 also stabilized midlife mortality rate increases so as to extend the maximum life span substantially beyond the limits previously reported for D. melanogaster. Under these conditions, SC100 also promoted robust resistance to partial starvation stress and to heat stress. Fertility was the same initially in both treated and control flies, but it became significantly higher in treated flies at older ages as the fertility of control flies declined. Mean and maximum life spans of flies in vials at the same test site were also extended by SC100, but the life spans were short in absolute terms. In contrast, at an independent test site where stress was minimized, the flies exhibited much longer mean life spans, but the survival curves became highly rectangular and the effects of SC100 on both mean and maximum life spans declined greatly or were abolished. The data indicate that SC100 is a novel herbal mix with striking effects on enhancing Drosophila stress resistance and life span in some environments, while minimizing mid to late life mortality rates. They also show that the environment and other factors can have transformative effects on both the length and distribution of survivorship, and on the ability of SC100 to extend the life span.

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Related in: MedlinePlus

Life span of Drosophila in cages with or without SC100 treatment.Nine independent cages were used in 9 simultaneous longevity assays, with each cage having 250 male and 250 female B4 flies at 25°C. Female or male sets of 3 cages each (Cages A, B, or C) are shown with the Survivorship (percent survival) on the Y axis and the Adult Age in days on the X axis. These data show Survivorship for: C) Control Untreated flies (green triangles), T) SC100 Treated flies (blue open squares), and CT) Control/Treated flies that were switched to SC100 treatment on day 36 of the assay (red diamonds). For each treatment, females in Fig 1A, 1B, and 1C were housed together with males in Fig 1D, 1E, and 1F, respectively.
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pone.0119068.g001: Life span of Drosophila in cages with or without SC100 treatment.Nine independent cages were used in 9 simultaneous longevity assays, with each cage having 250 male and 250 female B4 flies at 25°C. Female or male sets of 3 cages each (Cages A, B, or C) are shown with the Survivorship (percent survival) on the Y axis and the Adult Age in days on the X axis. These data show Survivorship for: C) Control Untreated flies (green triangles), T) SC100 Treated flies (blue open squares), and CT) Control/Treated flies that were switched to SC100 treatment on day 36 of the assay (red diamonds). For each treatment, females in Fig 1A, 1B, and 1C were housed together with males in Fig 1D, 1E, and 1F, respectively.

Mentions: In our large cage experiments, we used three cages with 250 males and 250 females per cage for each longevity screen, so that the longevity experiments were all done in triplicate cages. The three treatment conditions were: C) Control untreated flies; T) SC100 treated flies; and CT) Control/Treated flies that were switched from control conditions to SC100 treatment on day 36 of the longevity assay. Fig 1 shows female (Fig 1A–1C) and male (Fig 1D–1F) fly survivorship for each of the three replicate treatment conditions (Cages A, B, and C).


Herbal supplement extends life span under some environmental conditions and boosts stress resistance.

Villeponteau B, Matsagas K, Nobles AC, Rizza C, Horwitz M, Benford G, Mockett RJ - PLoS ONE (2015)

Life span of Drosophila in cages with or without SC100 treatment.Nine independent cages were used in 9 simultaneous longevity assays, with each cage having 250 male and 250 female B4 flies at 25°C. Female or male sets of 3 cages each (Cages A, B, or C) are shown with the Survivorship (percent survival) on the Y axis and the Adult Age in days on the X axis. These data show Survivorship for: C) Control Untreated flies (green triangles), T) SC100 Treated flies (blue open squares), and CT) Control/Treated flies that were switched to SC100 treatment on day 36 of the assay (red diamonds). For each treatment, females in Fig 1A, 1B, and 1C were housed together with males in Fig 1D, 1E, and 1F, respectively.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400117&req=5

pone.0119068.g001: Life span of Drosophila in cages with or without SC100 treatment.Nine independent cages were used in 9 simultaneous longevity assays, with each cage having 250 male and 250 female B4 flies at 25°C. Female or male sets of 3 cages each (Cages A, B, or C) are shown with the Survivorship (percent survival) on the Y axis and the Adult Age in days on the X axis. These data show Survivorship for: C) Control Untreated flies (green triangles), T) SC100 Treated flies (blue open squares), and CT) Control/Treated flies that were switched to SC100 treatment on day 36 of the assay (red diamonds). For each treatment, females in Fig 1A, 1B, and 1C were housed together with males in Fig 1D, 1E, and 1F, respectively.
Mentions: In our large cage experiments, we used three cages with 250 males and 250 females per cage for each longevity screen, so that the longevity experiments were all done in triplicate cages. The three treatment conditions were: C) Control untreated flies; T) SC100 treated flies; and CT) Control/Treated flies that were switched from control conditions to SC100 treatment on day 36 of the longevity assay. Fig 1 shows female (Fig 1A–1C) and male (Fig 1D–1F) fly survivorship for each of the three replicate treatment conditions (Cages A, B, and C).

Bottom Line: In contrast, at an independent test site where stress was minimized, the flies exhibited much longer mean life spans, but the survival curves became highly rectangular and the effects of SC100 on both mean and maximum life spans declined greatly or were abolished.The data indicate that SC100 is a novel herbal mix with striking effects on enhancing Drosophila stress resistance and life span in some environments, while minimizing mid to late life mortality rates.They also show that the environment and other factors can have transformative effects on both the length and distribution of survivorship, and on the ability of SC100 to extend the life span.

View Article: PubMed Central - PubMed

Affiliation: Genescient Inc., Fountain Valley, California, United States of America.

ABSTRACT
Genetic studies indicate that aging is modulated by a great number of genetic pathways. We have used Drosophila longevity and stress assays to test a multipath intervention strategy. To carry out this strategy, we supplemented the flies with herbal extracts (SC100) that are predicted to modulate the expression of many genes involved in aging and stress resistance, such as mTOR, NOS, NF-KappaB, and VEGF. When flies were housed in large cages with SC100 added, daily mortality rates of both male and female flies were greatly diminished in mid to late life. Surprisingly, SC100 also stabilized midlife mortality rate increases so as to extend the maximum life span substantially beyond the limits previously reported for D. melanogaster. Under these conditions, SC100 also promoted robust resistance to partial starvation stress and to heat stress. Fertility was the same initially in both treated and control flies, but it became significantly higher in treated flies at older ages as the fertility of control flies declined. Mean and maximum life spans of flies in vials at the same test site were also extended by SC100, but the life spans were short in absolute terms. In contrast, at an independent test site where stress was minimized, the flies exhibited much longer mean life spans, but the survival curves became highly rectangular and the effects of SC100 on both mean and maximum life spans declined greatly or were abolished. The data indicate that SC100 is a novel herbal mix with striking effects on enhancing Drosophila stress resistance and life span in some environments, while minimizing mid to late life mortality rates. They also show that the environment and other factors can have transformative effects on both the length and distribution of survivorship, and on the ability of SC100 to extend the life span.

Show MeSH
Related in: MedlinePlus