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Aluminium uptake and translocation in Al hyperaccumulator Rumex obtusifolius is affected by low-molecular-weight organic acids content and soil pH.

Vondráčková S, Száková J, Drábek O, Tejnecký V, Hejcman M, Müllerová V, Tlustoš P - PLoS ONE (2015)

Bottom Line: Elevated transport of total Al from belowground organs into leaves was recorded in both lime-treated soils and in superphosphate-treated alkaline soil as a result of sufficient amount of Ca available from soil solution as well as from superphosphate that can probably modify distribution of total Al in R. obtusifolius as a representative of "oxalate plants." The highest concentrations of Al and organic acids were recorded in the leaves, followed by the stem and belowground organ infusions.In alkaline soil, R. obtusifolius is an Al-hyperaccumulator with the highest concentrations of oxalate in leaves, of malate in stems, and of citrate in belowground organs.These organic acids form strong complexes with Al that can play a key role in internal Al tolerance but the used methods did not allow us to distinguish the proportion of total Al-organic complexes to the free organic acids.

View Article: PubMed Central - PubMed

Affiliation: Department of Agroenvironmental Chemistry and Plant Nutrition, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Kamýcká 129, 165 21 Prague 6, Suchdol, Czech Republic.

ABSTRACT

Background and aims: High Al resistance of Rumex obtusifolius together with its ability to accumulate Al has never been studied in weakly acidic conditions (pH > 5.8) and is not sufficiently described in real soil conditions. The potential elucidation of the role of organic acids in plant can explain the Al tolerance mechanism.

Methods: We established a pot experiment with R. obtusifolius planted in slightly acidic and alkaline soils. For the manipulation of Al availability, both soils were untreated and treated by lime and superphosphate. We determined mobile Al concentrations in soils and concentrations of Al and organic acids in organs.

Results: Al availability correlated positively to the extraction of organic acids (citric acid < oxalic acid) in soils. Monovalent Al cations were the most abundant mobile Al forms with positive charge in soils. Liming and superphosphate application were ambiguous measures for changing Al mobility in soils. Elevated transport of total Al from belowground organs into leaves was recorded in both lime-treated soils and in superphosphate-treated alkaline soil as a result of sufficient amount of Ca available from soil solution as well as from superphosphate that can probably modify distribution of total Al in R. obtusifolius as a representative of "oxalate plants." The highest concentrations of Al and organic acids were recorded in the leaves, followed by the stem and belowground organ infusions.

Conclusions: In alkaline soil, R. obtusifolius is an Al-hyperaccumulator with the highest concentrations of oxalate in leaves, of malate in stems, and of citrate in belowground organs. These organic acids form strong complexes with Al that can play a key role in internal Al tolerance but the used methods did not allow us to distinguish the proportion of total Al-organic complexes to the free organic acids.

No MeSH data available.


Related in: MedlinePlus

Ordination diagram showing the results of PCA analysis with total concentration of Al in soil extracts in contaminated slightly acidic Litavka and alkaline Malín soils.Treatment abbreviations: C—control, Ca—lime application, P—superphosphate application. Soil extractant abbreviations: KCl—exchangeable concentration of Al in soil (extracted by 0.5 mol/L KCl), CaCl2—exchangeable concentration of Al in soil (extracted by 0.01 mol/L CaCl2), H2O —water-soluble concentration of Al in soil (extracted by deionised water), AA—exchangeable and carbonate concentration of Al in soil (extracted by 0.11 mol/L acetic acid), CA—exchangeable, carbonate, and reducible concentration of Al in soil (extracted by 0.11 mol/L citric acid), and OA—exchangeable, carbonate, and reducible concentration of Al in soil (extracted by 0.11 mol/L oxalic acid).
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pone.0123351.g001: Ordination diagram showing the results of PCA analysis with total concentration of Al in soil extracts in contaminated slightly acidic Litavka and alkaline Malín soils.Treatment abbreviations: C—control, Ca—lime application, P—superphosphate application. Soil extractant abbreviations: KCl—exchangeable concentration of Al in soil (extracted by 0.5 mol/L KCl), CaCl2—exchangeable concentration of Al in soil (extracted by 0.01 mol/L CaCl2), H2O —water-soluble concentration of Al in soil (extracted by deionised water), AA—exchangeable and carbonate concentration of Al in soil (extracted by 0.11 mol/L acetic acid), CA—exchangeable, carbonate, and reducible concentration of Al in soil (extracted by 0.11 mol/L citric acid), and OA—exchangeable, carbonate, and reducible concentration of Al in soil (extracted by 0.11 mol/L oxalic acid).

Mentions: The efficiency of KCl and CaCl2 for extractability of Al was comparable in both soils (LC, LCa, LP, MC, MCa, and MP treatments). Higher efficiency of H2O and AA for Al extractability was recorded in Litavka soil (LC, LCa, and LP treatments) and of CA and OA was recorded in Malín soil (MC, MCa, and MP treatments; see Fig 1). A significant negative relationship was recorded between the concentration of Al extracted by H2O and CA (r = –0.947; p<0.01), by H2O and OA (r = –0.944; p<0.01), by AA and CA (r = –0.549; p<0.01), and by AA and OA (r = –0.562; p<0.01). A significant positive relationship was recorded between concentrations of Al extracted by H2O and AA (r = 0.408; p = 0.043) and by CA and OA (r = 0.997; p<0.01).


Aluminium uptake and translocation in Al hyperaccumulator Rumex obtusifolius is affected by low-molecular-weight organic acids content and soil pH.

Vondráčková S, Száková J, Drábek O, Tejnecký V, Hejcman M, Müllerová V, Tlustoš P - PLoS ONE (2015)

Ordination diagram showing the results of PCA analysis with total concentration of Al in soil extracts in contaminated slightly acidic Litavka and alkaline Malín soils.Treatment abbreviations: C—control, Ca—lime application, P—superphosphate application. Soil extractant abbreviations: KCl—exchangeable concentration of Al in soil (extracted by 0.5 mol/L KCl), CaCl2—exchangeable concentration of Al in soil (extracted by 0.01 mol/L CaCl2), H2O —water-soluble concentration of Al in soil (extracted by deionised water), AA—exchangeable and carbonate concentration of Al in soil (extracted by 0.11 mol/L acetic acid), CA—exchangeable, carbonate, and reducible concentration of Al in soil (extracted by 0.11 mol/L citric acid), and OA—exchangeable, carbonate, and reducible concentration of Al in soil (extracted by 0.11 mol/L oxalic acid).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400109&req=5

pone.0123351.g001: Ordination diagram showing the results of PCA analysis with total concentration of Al in soil extracts in contaminated slightly acidic Litavka and alkaline Malín soils.Treatment abbreviations: C—control, Ca—lime application, P—superphosphate application. Soil extractant abbreviations: KCl—exchangeable concentration of Al in soil (extracted by 0.5 mol/L KCl), CaCl2—exchangeable concentration of Al in soil (extracted by 0.01 mol/L CaCl2), H2O —water-soluble concentration of Al in soil (extracted by deionised water), AA—exchangeable and carbonate concentration of Al in soil (extracted by 0.11 mol/L acetic acid), CA—exchangeable, carbonate, and reducible concentration of Al in soil (extracted by 0.11 mol/L citric acid), and OA—exchangeable, carbonate, and reducible concentration of Al in soil (extracted by 0.11 mol/L oxalic acid).
Mentions: The efficiency of KCl and CaCl2 for extractability of Al was comparable in both soils (LC, LCa, LP, MC, MCa, and MP treatments). Higher efficiency of H2O and AA for Al extractability was recorded in Litavka soil (LC, LCa, and LP treatments) and of CA and OA was recorded in Malín soil (MC, MCa, and MP treatments; see Fig 1). A significant negative relationship was recorded between the concentration of Al extracted by H2O and CA (r = –0.947; p<0.01), by H2O and OA (r = –0.944; p<0.01), by AA and CA (r = –0.549; p<0.01), and by AA and OA (r = –0.562; p<0.01). A significant positive relationship was recorded between concentrations of Al extracted by H2O and AA (r = 0.408; p = 0.043) and by CA and OA (r = 0.997; p<0.01).

Bottom Line: Elevated transport of total Al from belowground organs into leaves was recorded in both lime-treated soils and in superphosphate-treated alkaline soil as a result of sufficient amount of Ca available from soil solution as well as from superphosphate that can probably modify distribution of total Al in R. obtusifolius as a representative of "oxalate plants." The highest concentrations of Al and organic acids were recorded in the leaves, followed by the stem and belowground organ infusions.In alkaline soil, R. obtusifolius is an Al-hyperaccumulator with the highest concentrations of oxalate in leaves, of malate in stems, and of citrate in belowground organs.These organic acids form strong complexes with Al that can play a key role in internal Al tolerance but the used methods did not allow us to distinguish the proportion of total Al-organic complexes to the free organic acids.

View Article: PubMed Central - PubMed

Affiliation: Department of Agroenvironmental Chemistry and Plant Nutrition, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Kamýcká 129, 165 21 Prague 6, Suchdol, Czech Republic.

ABSTRACT

Background and aims: High Al resistance of Rumex obtusifolius together with its ability to accumulate Al has never been studied in weakly acidic conditions (pH > 5.8) and is not sufficiently described in real soil conditions. The potential elucidation of the role of organic acids in plant can explain the Al tolerance mechanism.

Methods: We established a pot experiment with R. obtusifolius planted in slightly acidic and alkaline soils. For the manipulation of Al availability, both soils were untreated and treated by lime and superphosphate. We determined mobile Al concentrations in soils and concentrations of Al and organic acids in organs.

Results: Al availability correlated positively to the extraction of organic acids (citric acid < oxalic acid) in soils. Monovalent Al cations were the most abundant mobile Al forms with positive charge in soils. Liming and superphosphate application were ambiguous measures for changing Al mobility in soils. Elevated transport of total Al from belowground organs into leaves was recorded in both lime-treated soils and in superphosphate-treated alkaline soil as a result of sufficient amount of Ca available from soil solution as well as from superphosphate that can probably modify distribution of total Al in R. obtusifolius as a representative of "oxalate plants." The highest concentrations of Al and organic acids were recorded in the leaves, followed by the stem and belowground organ infusions.

Conclusions: In alkaline soil, R. obtusifolius is an Al-hyperaccumulator with the highest concentrations of oxalate in leaves, of malate in stems, and of citrate in belowground organs. These organic acids form strong complexes with Al that can play a key role in internal Al tolerance but the used methods did not allow us to distinguish the proportion of total Al-organic complexes to the free organic acids.

No MeSH data available.


Related in: MedlinePlus