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Plasma microRNA levels differ between endurance and strength athletes.

Wardle SL, Bailey ME, Kilikevicius A, Malkova D, Wilson RH, Venckunas T, Moran CN - PLoS ONE (2015)

Bottom Line: Moreover, we investigated whether c-miRNA levels were associated with quantitative performance-related phenotypes within and between groups. miR-222 was present at different levels in the three participant groups (p = 0.028) with the highest levels being observed in END and the lowest in STR.Certain c-miRNAs (miR-222, miR-21, miR-146a and miR-221) differ between endurance- and resistance-trained athletes and thus have potential as useful biomarkers of exercise training and / or play a role in exercise mode-specific training adaptations.However, levels of these c-miRNAs are probably unrelated to muscle bulk or fat reserves.

View Article: PubMed Central - PubMed

Affiliation: Health and Exercise Sciences Research Group, University of Stirling, Stirling, Scotland.

ABSTRACT

Aim: MicroRNAs (miRNAs) are stable in the circulation and are likely to function in inter-organ communication during a variety of metabolic responses that involve changes in gene expression, including exercise training. However, it is unknown whether differences in circulating-miRNA (c-miRNA) levels are characteristic of training modality.

Methods: We investigated whether levels of candidate c-miRNAs differ between elite male athletes of two different training modalities (n = 10 per group)--endurance (END) and strength (STR)--and between these groups and untrained controls (CON; n = 10). Fasted, non-exercised, morning plasma samples were analysed for 14 c-miRNAs (miR-1, miR-16-2, miR-20a-1, miR-21, miR-93, miR-103a, miR-133a, miR-146a, miR-192, miR-206, miR-221, miR-222, miR-451, miR-499). Moreover, we investigated whether c-miRNA levels were associated with quantitative performance-related phenotypes within and between groups.

Results: miR-222 was present at different levels in the three participant groups (p = 0.028) with the highest levels being observed in END and the lowest in STR. A number of other c-miRNAs were present at higher levels in END than in STR (relative to STR, ± 1 SEM; miR-222: 1.94 fold (1.73-2.18), p = 0.011; miR-21: 1.56 fold (1.39-1.74), p = 0.013; miR-146a: 1.50 fold (1.38-1.64), p = 0.019; miR-221: 1.51 fold (1.34-1.70), p = 0.026). Regression analyses revealed several associations between candidate c-miRNA levels and strength-related performance measures before and after adjustment for muscle or fat mass, but not following adjustment for group.

Conclusion: Certain c-miRNAs (miR-222, miR-21, miR-146a and miR-221) differ between endurance- and resistance-trained athletes and thus have potential as useful biomarkers of exercise training and / or play a role in exercise mode-specific training adaptations. However, levels of these c-miRNAs are probably unrelated to muscle bulk or fat reserves.

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Related in: MedlinePlus

Relative expression levels of miR-222 (A), -21 (B), -146a (C), and -221 (D) in STR, CON and END.Box plots depict the range (upper and lower whiskers), median (centre line) and interquartile range (edge of boxes). * significantly different between all groups (One way ANOVA; p < 0.05); † significantly different from STR (t-test; p < 0.05).
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pone.0122107.g001: Relative expression levels of miR-222 (A), -21 (B), -146a (C), and -221 (D) in STR, CON and END.Box plots depict the range (upper and lower whiskers), median (centre line) and interquartile range (edge of boxes). * significantly different between all groups (One way ANOVA; p < 0.05); † significantly different from STR (t-test; p < 0.05).

Mentions: Circulating levels of 14 miRNAs were measured in the athlete and control groups. Table 2 shows mean relative levels for each group for each of the test miRNAs, standardised to the reference gene (miR-93) and to levels in the control (CON) group. One-way ANOVA revealed a statistically significant difference (p = 0.028; Fig 1A) in miR-222 levels between the three groups, with levels nominally being higher than CON in the endurance (END) group and lower than CON in the strength (STR) group. The post-hoc Tukey’s test revealed a significant difference between STR and END (p = 0.011).


Plasma microRNA levels differ between endurance and strength athletes.

Wardle SL, Bailey ME, Kilikevicius A, Malkova D, Wilson RH, Venckunas T, Moran CN - PLoS ONE (2015)

Relative expression levels of miR-222 (A), -21 (B), -146a (C), and -221 (D) in STR, CON and END.Box plots depict the range (upper and lower whiskers), median (centre line) and interquartile range (edge of boxes). * significantly different between all groups (One way ANOVA; p < 0.05); † significantly different from STR (t-test; p < 0.05).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400105&req=5

pone.0122107.g001: Relative expression levels of miR-222 (A), -21 (B), -146a (C), and -221 (D) in STR, CON and END.Box plots depict the range (upper and lower whiskers), median (centre line) and interquartile range (edge of boxes). * significantly different between all groups (One way ANOVA; p < 0.05); † significantly different from STR (t-test; p < 0.05).
Mentions: Circulating levels of 14 miRNAs were measured in the athlete and control groups. Table 2 shows mean relative levels for each group for each of the test miRNAs, standardised to the reference gene (miR-93) and to levels in the control (CON) group. One-way ANOVA revealed a statistically significant difference (p = 0.028; Fig 1A) in miR-222 levels between the three groups, with levels nominally being higher than CON in the endurance (END) group and lower than CON in the strength (STR) group. The post-hoc Tukey’s test revealed a significant difference between STR and END (p = 0.011).

Bottom Line: Moreover, we investigated whether c-miRNA levels were associated with quantitative performance-related phenotypes within and between groups. miR-222 was present at different levels in the three participant groups (p = 0.028) with the highest levels being observed in END and the lowest in STR.Certain c-miRNAs (miR-222, miR-21, miR-146a and miR-221) differ between endurance- and resistance-trained athletes and thus have potential as useful biomarkers of exercise training and / or play a role in exercise mode-specific training adaptations.However, levels of these c-miRNAs are probably unrelated to muscle bulk or fat reserves.

View Article: PubMed Central - PubMed

Affiliation: Health and Exercise Sciences Research Group, University of Stirling, Stirling, Scotland.

ABSTRACT

Aim: MicroRNAs (miRNAs) are stable in the circulation and are likely to function in inter-organ communication during a variety of metabolic responses that involve changes in gene expression, including exercise training. However, it is unknown whether differences in circulating-miRNA (c-miRNA) levels are characteristic of training modality.

Methods: We investigated whether levels of candidate c-miRNAs differ between elite male athletes of two different training modalities (n = 10 per group)--endurance (END) and strength (STR)--and between these groups and untrained controls (CON; n = 10). Fasted, non-exercised, morning plasma samples were analysed for 14 c-miRNAs (miR-1, miR-16-2, miR-20a-1, miR-21, miR-93, miR-103a, miR-133a, miR-146a, miR-192, miR-206, miR-221, miR-222, miR-451, miR-499). Moreover, we investigated whether c-miRNA levels were associated with quantitative performance-related phenotypes within and between groups.

Results: miR-222 was present at different levels in the three participant groups (p = 0.028) with the highest levels being observed in END and the lowest in STR. A number of other c-miRNAs were present at higher levels in END than in STR (relative to STR, ± 1 SEM; miR-222: 1.94 fold (1.73-2.18), p = 0.011; miR-21: 1.56 fold (1.39-1.74), p = 0.013; miR-146a: 1.50 fold (1.38-1.64), p = 0.019; miR-221: 1.51 fold (1.34-1.70), p = 0.026). Regression analyses revealed several associations between candidate c-miRNA levels and strength-related performance measures before and after adjustment for muscle or fat mass, but not following adjustment for group.

Conclusion: Certain c-miRNAs (miR-222, miR-21, miR-146a and miR-221) differ between endurance- and resistance-trained athletes and thus have potential as useful biomarkers of exercise training and / or play a role in exercise mode-specific training adaptations. However, levels of these c-miRNAs are probably unrelated to muscle bulk or fat reserves.

Show MeSH
Related in: MedlinePlus