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Characteristics of human amniotic fluid mesenchymal stem cells and their tropism to human ovarian cancer.

Li L, Wang D, Zhou J, Cheng Y, Liang T, Zhang G - PLoS ONE (2015)

Bottom Line: We successfully isolated the AFMSCs from thirteen of twenty amniotic fluid samples.RT-PCR results showed that the AFMSCs expressed stem cell marker OCT4.Meanwhile, the research emphasis in the future can also put in targeting therapy of ovarian cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Genecology and Obstetrics, Harbin Medical University, Harbin, Heilongjiang, China.

ABSTRACT
The mesenchymal stem cells (MSCs) derived from amniotic fluid (AF) have become an attractive stem cells source for cell-based therapy because they can be harvested at low cost and avoid ethical disputes. In human research, stem cells derived from AF gradually became a hot research direction for disease treatment, specifically for their plasticity, their reduced immunogenicity and their tumor tropism regardless of the tumor size, location and source. Our work aimed to obtain and characterize human amniotic fluid mesenchymal stem cells (AFMSCs) and detect their ovarian cancer tropsim in nude mice model. Ten milliliters of twenty independent amniotic fluid samples were collected from 16-20 week pregnant women who underwent amniocentesis for fetal genetic determination in routine prenatal diagnosis in the first affiliated hospital of Harbin medical university. We successfully isolated the AFMSCs from thirteen of twenty amniotic fluid samples. AFMSCs presented a fibroblastic-like morphology during the culture. Flow cytometry analyses showed that the cells were positive for specific stem cell markers CD73,CD90, CD105, CD166 and HLA-ABC (MHC class I), but negative for CD 45,CD40, CD34, CD14 and HLA-DR (MHC class II). RT-PCR results showed that the AFMSCs expressed stem cell marker OCT4. AFMSCs could differentiate into bone cells, fat cells and chondrocytes under certain conditions. AFMSCs had the high motility to migrate to ovarian cancer site but didn't have the tumorigenicity. This study enhances the possibility of AFMSCs as drug carrier in human cell-based therapy. Meanwhile, the research emphasis in the future can also put in targeting therapy of ovarian cancer.

No MeSH data available.


Related in: MedlinePlus

Immunophenotypes of AFMSCs.Flow cytometry analysis showed that the AFMSCs positively expressed CD73 CD90, CD105 and CD166, but negatively expressed CD34, CD45 and CD14. The results also showed that the AFMSCs expressed HLA-ABC(MHC class I), but not HLA-DR(MHC class II) nor CD40.
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pone.0123350.g004: Immunophenotypes of AFMSCs.Flow cytometry analysis showed that the AFMSCs positively expressed CD73 CD90, CD105 and CD166, but negatively expressed CD34, CD45 and CD14. The results also showed that the AFMSCs expressed HLA-ABC(MHC class I), but not HLA-DR(MHC class II) nor CD40.

Mentions: Based on Flow cytometry analysis, we found that the AFMSCs were positive for CD166, CD90,CD73 and CD105, but negative for CD34, CD45 and CD14, representing characteristic phenotypes of mesenchymal stem cells. The results also showed that the AFMSCs expressed HLA-ABC(MHC class I), but not HLA-DR(MHC class II), nor CD40, manifesting the low immunity of AFMSCs (Fig 4).


Characteristics of human amniotic fluid mesenchymal stem cells and their tropism to human ovarian cancer.

Li L, Wang D, Zhou J, Cheng Y, Liang T, Zhang G - PLoS ONE (2015)

Immunophenotypes of AFMSCs.Flow cytometry analysis showed that the AFMSCs positively expressed CD73 CD90, CD105 and CD166, but negatively expressed CD34, CD45 and CD14. The results also showed that the AFMSCs expressed HLA-ABC(MHC class I), but not HLA-DR(MHC class II) nor CD40.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400015&req=5

pone.0123350.g004: Immunophenotypes of AFMSCs.Flow cytometry analysis showed that the AFMSCs positively expressed CD73 CD90, CD105 and CD166, but negatively expressed CD34, CD45 and CD14. The results also showed that the AFMSCs expressed HLA-ABC(MHC class I), but not HLA-DR(MHC class II) nor CD40.
Mentions: Based on Flow cytometry analysis, we found that the AFMSCs were positive for CD166, CD90,CD73 and CD105, but negative for CD34, CD45 and CD14, representing characteristic phenotypes of mesenchymal stem cells. The results also showed that the AFMSCs expressed HLA-ABC(MHC class I), but not HLA-DR(MHC class II), nor CD40, manifesting the low immunity of AFMSCs (Fig 4).

Bottom Line: We successfully isolated the AFMSCs from thirteen of twenty amniotic fluid samples.RT-PCR results showed that the AFMSCs expressed stem cell marker OCT4.Meanwhile, the research emphasis in the future can also put in targeting therapy of ovarian cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Genecology and Obstetrics, Harbin Medical University, Harbin, Heilongjiang, China.

ABSTRACT
The mesenchymal stem cells (MSCs) derived from amniotic fluid (AF) have become an attractive stem cells source for cell-based therapy because they can be harvested at low cost and avoid ethical disputes. In human research, stem cells derived from AF gradually became a hot research direction for disease treatment, specifically for their plasticity, their reduced immunogenicity and their tumor tropism regardless of the tumor size, location and source. Our work aimed to obtain and characterize human amniotic fluid mesenchymal stem cells (AFMSCs) and detect their ovarian cancer tropsim in nude mice model. Ten milliliters of twenty independent amniotic fluid samples were collected from 16-20 week pregnant women who underwent amniocentesis for fetal genetic determination in routine prenatal diagnosis in the first affiliated hospital of Harbin medical university. We successfully isolated the AFMSCs from thirteen of twenty amniotic fluid samples. AFMSCs presented a fibroblastic-like morphology during the culture. Flow cytometry analyses showed that the cells were positive for specific stem cell markers CD73,CD90, CD105, CD166 and HLA-ABC (MHC class I), but negative for CD 45,CD40, CD34, CD14 and HLA-DR (MHC class II). RT-PCR results showed that the AFMSCs expressed stem cell marker OCT4. AFMSCs could differentiate into bone cells, fat cells and chondrocytes under certain conditions. AFMSCs had the high motility to migrate to ovarian cancer site but didn't have the tumorigenicity. This study enhances the possibility of AFMSCs as drug carrier in human cell-based therapy. Meanwhile, the research emphasis in the future can also put in targeting therapy of ovarian cancer.

No MeSH data available.


Related in: MedlinePlus