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Characteristics of human amniotic fluid mesenchymal stem cells and their tropism to human ovarian cancer.

Li L, Wang D, Zhou J, Cheng Y, Liang T, Zhang G - PLoS ONE (2015)

Bottom Line: We successfully isolated the AFMSCs from thirteen of twenty amniotic fluid samples.RT-PCR results showed that the AFMSCs expressed stem cell marker OCT4.Meanwhile, the research emphasis in the future can also put in targeting therapy of ovarian cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Genecology and Obstetrics, Harbin Medical University, Harbin, Heilongjiang, China.

ABSTRACT
The mesenchymal stem cells (MSCs) derived from amniotic fluid (AF) have become an attractive stem cells source for cell-based therapy because they can be harvested at low cost and avoid ethical disputes. In human research, stem cells derived from AF gradually became a hot research direction for disease treatment, specifically for their plasticity, their reduced immunogenicity and their tumor tropism regardless of the tumor size, location and source. Our work aimed to obtain and characterize human amniotic fluid mesenchymal stem cells (AFMSCs) and detect their ovarian cancer tropsim in nude mice model. Ten milliliters of twenty independent amniotic fluid samples were collected from 16-20 week pregnant women who underwent amniocentesis for fetal genetic determination in routine prenatal diagnosis in the first affiliated hospital of Harbin medical university. We successfully isolated the AFMSCs from thirteen of twenty amniotic fluid samples. AFMSCs presented a fibroblastic-like morphology during the culture. Flow cytometry analyses showed that the cells were positive for specific stem cell markers CD73,CD90, CD105, CD166 and HLA-ABC (MHC class I), but negative for CD 45,CD40, CD34, CD14 and HLA-DR (MHC class II). RT-PCR results showed that the AFMSCs expressed stem cell marker OCT4. AFMSCs could differentiate into bone cells, fat cells and chondrocytes under certain conditions. AFMSCs had the high motility to migrate to ovarian cancer site but didn't have the tumorigenicity. This study enhances the possibility of AFMSCs as drug carrier in human cell-based therapy. Meanwhile, the research emphasis in the future can also put in targeting therapy of ovarian cancer.

No MeSH data available.


Related in: MedlinePlus

Morphology of different passages of AFMSCs (Scale bar = 1000um).(A)The non adherent cells; (B) The primary passage; (C) The fifth passage;(D) The tenth passage;(E)The fifteenth passage; (F) The twentieth passage.
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pone.0123350.g001: Morphology of different passages of AFMSCs (Scale bar = 1000um).(A)The non adherent cells; (B) The primary passage; (C) The fifth passage;(D) The tenth passage;(E)The fifteenth passage; (F) The twentieth passage.

Mentions: AFMSCs were successfully isolated through the centrifugal adherent method from thirteen of twenty amniotic fluid samples. Primary stem cells collected from the second trimester of pregnancy amniotic fluid adhered to plates at 5–7 days later and presented short polygon or fusiform. About 10 days later, part of the cells turned globular or strip. The cell body assumed spindle. With the rapid proliferation of cells, we observed more cell clones appeared. Three weeks later the cells reached 80%-90% confluency. At this time these cells presented relatively uniform long spindle which were similar to bone marrow mesenchymal stem cells. With the increase of cell passage, the shape of cells became slender. Cells could extend continuously to 20 generations and after the 20th generation cell morphological began to change, cell body increased, arranged loosely and cell edge turned burr shaped.(Fig 1)


Characteristics of human amniotic fluid mesenchymal stem cells and their tropism to human ovarian cancer.

Li L, Wang D, Zhou J, Cheng Y, Liang T, Zhang G - PLoS ONE (2015)

Morphology of different passages of AFMSCs (Scale bar = 1000um).(A)The non adherent cells; (B) The primary passage; (C) The fifth passage;(D) The tenth passage;(E)The fifteenth passage; (F) The twentieth passage.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400015&req=5

pone.0123350.g001: Morphology of different passages of AFMSCs (Scale bar = 1000um).(A)The non adherent cells; (B) The primary passage; (C) The fifth passage;(D) The tenth passage;(E)The fifteenth passage; (F) The twentieth passage.
Mentions: AFMSCs were successfully isolated through the centrifugal adherent method from thirteen of twenty amniotic fluid samples. Primary stem cells collected from the second trimester of pregnancy amniotic fluid adhered to plates at 5–7 days later and presented short polygon or fusiform. About 10 days later, part of the cells turned globular or strip. The cell body assumed spindle. With the rapid proliferation of cells, we observed more cell clones appeared. Three weeks later the cells reached 80%-90% confluency. At this time these cells presented relatively uniform long spindle which were similar to bone marrow mesenchymal stem cells. With the increase of cell passage, the shape of cells became slender. Cells could extend continuously to 20 generations and after the 20th generation cell morphological began to change, cell body increased, arranged loosely and cell edge turned burr shaped.(Fig 1)

Bottom Line: We successfully isolated the AFMSCs from thirteen of twenty amniotic fluid samples.RT-PCR results showed that the AFMSCs expressed stem cell marker OCT4.Meanwhile, the research emphasis in the future can also put in targeting therapy of ovarian cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Genecology and Obstetrics, Harbin Medical University, Harbin, Heilongjiang, China.

ABSTRACT
The mesenchymal stem cells (MSCs) derived from amniotic fluid (AF) have become an attractive stem cells source for cell-based therapy because they can be harvested at low cost and avoid ethical disputes. In human research, stem cells derived from AF gradually became a hot research direction for disease treatment, specifically for their plasticity, their reduced immunogenicity and their tumor tropism regardless of the tumor size, location and source. Our work aimed to obtain and characterize human amniotic fluid mesenchymal stem cells (AFMSCs) and detect their ovarian cancer tropsim in nude mice model. Ten milliliters of twenty independent amniotic fluid samples were collected from 16-20 week pregnant women who underwent amniocentesis for fetal genetic determination in routine prenatal diagnosis in the first affiliated hospital of Harbin medical university. We successfully isolated the AFMSCs from thirteen of twenty amniotic fluid samples. AFMSCs presented a fibroblastic-like morphology during the culture. Flow cytometry analyses showed that the cells were positive for specific stem cell markers CD73,CD90, CD105, CD166 and HLA-ABC (MHC class I), but negative for CD 45,CD40, CD34, CD14 and HLA-DR (MHC class II). RT-PCR results showed that the AFMSCs expressed stem cell marker OCT4. AFMSCs could differentiate into bone cells, fat cells and chondrocytes under certain conditions. AFMSCs had the high motility to migrate to ovarian cancer site but didn't have the tumorigenicity. This study enhances the possibility of AFMSCs as drug carrier in human cell-based therapy. Meanwhile, the research emphasis in the future can also put in targeting therapy of ovarian cancer.

No MeSH data available.


Related in: MedlinePlus