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Performance of Hitchens-Pike-Todd-Hewitt medium for group B streptococcus screening in pregnant women.

de Melo SC, Gavena AA, Silva FT, Moreira RC, de Lima Scodro RB, Cardoso RF, Siqueira VL, de Pádua RA, Carvalho MD, Pelloso SM - PLoS ONE (2015)

Bottom Line: Vaginal and anorectal clinical specimens from each pregnant woman were plated on sheep blood agar (SBA) and seeded on HPTH medium and Todd-Hewitt enrichment broth.The GBS colonization rates that were detected by each medium were 22.2% for HPTH medium, 21.2% for SBA, and 13.1% for Todd-Hewitt enrichment broth.Of the 60 pregnant women at ≥ 38 weeks of gestation, seven (11.7%) were positive for GBS.

View Article: PubMed Central - PubMed

Affiliation: Postgraduate Program in Health Sciences, State University of Maringá, Maringá, Paraná, Brazil; Nursing Sector, State University of Northern Paraná, Bandeirantes, Paraná, Brazil.

ABSTRACT
Group B streptococcus (GBS), which commonly colonizes the female genital tract and rectum, can cause infections in newborns with varying severity, possibly leading to death. The aim of the present study was to evaluate Hitchens-Pike-Todd-Hewitt (HPTH) medium performance for GBS screening in pregnant women. A descriptive analytical cross-sectional study was performed with 556 pregnant women, of which 496 were at 35-37 weeks of gestation and 60 were at ≥ 38 weeks of gestation. The study was conducted from September 2011 to March 2014 in northern Paraná, Brazil. Vaginal and anorectal clinical specimens from each pregnant woman were plated on sheep blood agar (SBA) and seeded on HPTH medium and Todd-Hewitt enrichment broth. Of the 496 pregnant women at 35-37 weeks of gestation, 141 (28.4%) were positive for GBS, based on the combination of the three culture media and clinical specimens. The GBS colonization rates that were detected by each medium were 22.2% for HPTH medium, 21.2% for SBA, and 13.1% for Todd-Hewitt enrichment broth. Of the 60 pregnant women at ≥ 38 weeks of gestation, seven (11.7%) were positive for GBS. These results demonstrate that HPTH medium and SBA were more sensitive than Todd-Hewitt enrichment broth for GBS screening in pregnant women and good GBS recovery in culture, indicating that the two media should be used together for vaginal and anorectal specimens.

No MeSH data available.


Related in: MedlinePlus

Number of Streptococcus agalactiae positive pregnant women (35–37 weeks of gestation) detected by HPTH medium, Todd-Hewitt enrichment broth (TODD), and sheep blood agar (SBA) plating in vaginal samples.
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pone.0123988.g001: Number of Streptococcus agalactiae positive pregnant women (35–37 weeks of gestation) detected by HPTH medium, Todd-Hewitt enrichment broth (TODD), and sheep blood agar (SBA) plating in vaginal samples.

Mentions: Of the 496 pregnant women at 35–37 weeks of gestation who participated in the study, 141 (28.4%) were positive for GBS based on the combination of the three culture media for the two clinical specimens (S1 Table). The GBS colonization rate detected was 22.2% for HPTH medium, 21.2% for SBA, and 13.1% for Todd-Hewitt enrichment broth (Table 1). Of the pregnant women who were positive for GBS, based on HPTH medium, 8.1% were positive exclusively in vaginal specimens, 3.8% were positive exclusively in anorectal specimens, and 10.3% were positive in both specimens. SBA plating revealed GBS-positive cultures in 5.2% of the vaginal specimens and 6.0% of the anorectal specimens, and 9.9% were positive in both specimens. Plating with Todd-Hewitt enrichment broth revealed GBS-positive cultures in 10.1% of the vaginal specimens and 1.4% of the anorectal specimens, and 1.6% were positive in both specimens (Figs 1 and 2, Tables 1 and 2).


Performance of Hitchens-Pike-Todd-Hewitt medium for group B streptococcus screening in pregnant women.

de Melo SC, Gavena AA, Silva FT, Moreira RC, de Lima Scodro RB, Cardoso RF, Siqueira VL, de Pádua RA, Carvalho MD, Pelloso SM - PLoS ONE (2015)

Number of Streptococcus agalactiae positive pregnant women (35–37 weeks of gestation) detected by HPTH medium, Todd-Hewitt enrichment broth (TODD), and sheep blood agar (SBA) plating in vaginal samples.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400012&req=5

pone.0123988.g001: Number of Streptococcus agalactiae positive pregnant women (35–37 weeks of gestation) detected by HPTH medium, Todd-Hewitt enrichment broth (TODD), and sheep blood agar (SBA) plating in vaginal samples.
Mentions: Of the 496 pregnant women at 35–37 weeks of gestation who participated in the study, 141 (28.4%) were positive for GBS based on the combination of the three culture media for the two clinical specimens (S1 Table). The GBS colonization rate detected was 22.2% for HPTH medium, 21.2% for SBA, and 13.1% for Todd-Hewitt enrichment broth (Table 1). Of the pregnant women who were positive for GBS, based on HPTH medium, 8.1% were positive exclusively in vaginal specimens, 3.8% were positive exclusively in anorectal specimens, and 10.3% were positive in both specimens. SBA plating revealed GBS-positive cultures in 5.2% of the vaginal specimens and 6.0% of the anorectal specimens, and 9.9% were positive in both specimens. Plating with Todd-Hewitt enrichment broth revealed GBS-positive cultures in 10.1% of the vaginal specimens and 1.4% of the anorectal specimens, and 1.6% were positive in both specimens (Figs 1 and 2, Tables 1 and 2).

Bottom Line: Vaginal and anorectal clinical specimens from each pregnant woman were plated on sheep blood agar (SBA) and seeded on HPTH medium and Todd-Hewitt enrichment broth.The GBS colonization rates that were detected by each medium were 22.2% for HPTH medium, 21.2% for SBA, and 13.1% for Todd-Hewitt enrichment broth.Of the 60 pregnant women at ≥ 38 weeks of gestation, seven (11.7%) were positive for GBS.

View Article: PubMed Central - PubMed

Affiliation: Postgraduate Program in Health Sciences, State University of Maringá, Maringá, Paraná, Brazil; Nursing Sector, State University of Northern Paraná, Bandeirantes, Paraná, Brazil.

ABSTRACT
Group B streptococcus (GBS), which commonly colonizes the female genital tract and rectum, can cause infections in newborns with varying severity, possibly leading to death. The aim of the present study was to evaluate Hitchens-Pike-Todd-Hewitt (HPTH) medium performance for GBS screening in pregnant women. A descriptive analytical cross-sectional study was performed with 556 pregnant women, of which 496 were at 35-37 weeks of gestation and 60 were at ≥ 38 weeks of gestation. The study was conducted from September 2011 to March 2014 in northern Paraná, Brazil. Vaginal and anorectal clinical specimens from each pregnant woman were plated on sheep blood agar (SBA) and seeded on HPTH medium and Todd-Hewitt enrichment broth. Of the 496 pregnant women at 35-37 weeks of gestation, 141 (28.4%) were positive for GBS, based on the combination of the three culture media and clinical specimens. The GBS colonization rates that were detected by each medium were 22.2% for HPTH medium, 21.2% for SBA, and 13.1% for Todd-Hewitt enrichment broth. Of the 60 pregnant women at ≥ 38 weeks of gestation, seven (11.7%) were positive for GBS. These results demonstrate that HPTH medium and SBA were more sensitive than Todd-Hewitt enrichment broth for GBS screening in pregnant women and good GBS recovery in culture, indicating that the two media should be used together for vaginal and anorectal specimens.

No MeSH data available.


Related in: MedlinePlus