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Proteomics perspectives in rotator cuff research: a systematic review of gene expression and protein composition in human tendinopathy.

Sejersen MH, Frost P, Hansen TB, Deutch SR, Svendsen SW - PLoS ONE (2015)

Bottom Line: There was a tendency towards an increase of collagen I (11 of 15 studies) and III (13 of 14), metalloproteinase (MMP)-1 (6 of 12), -9 (7 of 7), -13 (4 of 7), tissue inhibitor of metalloproteinase (TIMP)-1 (4 of 7), and vascular endothelial growth factor (4 of 7), and a decrease in MMP-3 (10 of 12).Fourteen proteomics studies of tendon tissues/cells failed inclusion, mostly because they were conducted in animals or in vitro.Thus, our results suggested an untapped potential for proteomics in tendon research.

View Article: PubMed Central - PubMed

Affiliation: Danish Ramazzini Centre, Department of Occupational Medicine, Regional Hospital West Jutland-University Research Clinic, Herning, Denmark.

ABSTRACT

Background: Rotator cuff tendinopathy including tears is a cause of significant morbidity. The molecular pathogenesis of the disorder is largely unknown. This review aimed to present an overview of the literature on gene expression and protein composition in human rotator cuff tendinopathy and other tendinopathies, and to evaluate perspectives of proteomics--the comprehensive study of protein composition--in tendon research.

Materials and methods: We conducted a systematic search of the literature published between 1 January 1990 and 18 December 2012 in PubMed, Embase, and Web of Science. We included studies on objectively quantified differential gene expression and/or protein composition in human rotator cuff tendinopathy and other tendinopathies as compared to control tissue.

Results: We identified 2199 studies, of which 54 were included; 25 studies focussed on rotator cuff or biceps tendinopathy. Most of the included studies quantified prespecified mRNA molecules and proteins using polymerase chain reactions and immunoassays, respectively. There was a tendency towards an increase of collagen I (11 of 15 studies) and III (13 of 14), metalloproteinase (MMP)-1 (6 of 12), -9 (7 of 7), -13 (4 of 7), tissue inhibitor of metalloproteinase (TIMP)-1 (4 of 7), and vascular endothelial growth factor (4 of 7), and a decrease in MMP-3 (10 of 12). Fourteen proteomics studies of tendon tissues/cells failed inclusion, mostly because they were conducted in animals or in vitro.

Conclusions: Based on methods, which only allowed simultaneous quantification of a limited number of prespecified mRNA molecules or proteins, several proteins appeared to be differentially expressed/represented in rotator cuff tendinopathy and other tendinopathies. No proteomics studies fulfilled our inclusion criteria, although proteomics technologies may be a way to identify protein profiles (including non-prespecified proteins) that characterise specific tendon disorders or stages of tendinopathy. Thus, our results suggested an untapped potential for proteomics in tendon research.

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Flow diagram showing the inclusion process.
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pone.0119974.g001: Flow diagram showing the inclusion process.

Mentions: The primary searches yielded 2199 articles, of which we excluded 2097 based on title or abstract and 48 after full-text reading (a list of these 48 articles and reasons for their exclusion can be found in S5 Appendix). Of the 54 included articles, 25 dealt with rotator cuff or biceps tendinopathy[17, 18, 36–58], 14 with Achilles tendinopathy[33, 59–71], 3 with posterior tibial tendinopathy[72–74], 9 with patellar tendinopathy[75–83], 1 with both Achilles and patellar tendinopathy[84], 1 with both Achilles tendinopathy and posterior tibial tendinopathy[85], and 1 with pooled tendinopathic tissue from various anatomical locations[86]. Fig 1 displays the flow diagram of the inclusion process. Table 2 summarises characteristics and findings of rotator cuff and biceps studies. S1–S3 Tables show corresponding information regarding other tendons. In total, the 54 included articles comprised 975 specimens representing tendinopathy with or without tears and 508 control samples, and they explicitly evaluated the expression/representation of more than 140 prespecified transcripts and proteins. Table 3 shows experimental techniques used for the analysis of gene expression and protein composition. The predominant laboratory methods were real-time quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) to detect and quantify mRNA, and Western blots and immunohistochemistry to detect and quantify proteins; only two studies used micro arrays.


Proteomics perspectives in rotator cuff research: a systematic review of gene expression and protein composition in human tendinopathy.

Sejersen MH, Frost P, Hansen TB, Deutch SR, Svendsen SW - PLoS ONE (2015)

Flow diagram showing the inclusion process.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400011&req=5

pone.0119974.g001: Flow diagram showing the inclusion process.
Mentions: The primary searches yielded 2199 articles, of which we excluded 2097 based on title or abstract and 48 after full-text reading (a list of these 48 articles and reasons for their exclusion can be found in S5 Appendix). Of the 54 included articles, 25 dealt with rotator cuff or biceps tendinopathy[17, 18, 36–58], 14 with Achilles tendinopathy[33, 59–71], 3 with posterior tibial tendinopathy[72–74], 9 with patellar tendinopathy[75–83], 1 with both Achilles and patellar tendinopathy[84], 1 with both Achilles tendinopathy and posterior tibial tendinopathy[85], and 1 with pooled tendinopathic tissue from various anatomical locations[86]. Fig 1 displays the flow diagram of the inclusion process. Table 2 summarises characteristics and findings of rotator cuff and biceps studies. S1–S3 Tables show corresponding information regarding other tendons. In total, the 54 included articles comprised 975 specimens representing tendinopathy with or without tears and 508 control samples, and they explicitly evaluated the expression/representation of more than 140 prespecified transcripts and proteins. Table 3 shows experimental techniques used for the analysis of gene expression and protein composition. The predominant laboratory methods were real-time quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) to detect and quantify mRNA, and Western blots and immunohistochemistry to detect and quantify proteins; only two studies used micro arrays.

Bottom Line: There was a tendency towards an increase of collagen I (11 of 15 studies) and III (13 of 14), metalloproteinase (MMP)-1 (6 of 12), -9 (7 of 7), -13 (4 of 7), tissue inhibitor of metalloproteinase (TIMP)-1 (4 of 7), and vascular endothelial growth factor (4 of 7), and a decrease in MMP-3 (10 of 12).Fourteen proteomics studies of tendon tissues/cells failed inclusion, mostly because they were conducted in animals or in vitro.Thus, our results suggested an untapped potential for proteomics in tendon research.

View Article: PubMed Central - PubMed

Affiliation: Danish Ramazzini Centre, Department of Occupational Medicine, Regional Hospital West Jutland-University Research Clinic, Herning, Denmark.

ABSTRACT

Background: Rotator cuff tendinopathy including tears is a cause of significant morbidity. The molecular pathogenesis of the disorder is largely unknown. This review aimed to present an overview of the literature on gene expression and protein composition in human rotator cuff tendinopathy and other tendinopathies, and to evaluate perspectives of proteomics--the comprehensive study of protein composition--in tendon research.

Materials and methods: We conducted a systematic search of the literature published between 1 January 1990 and 18 December 2012 in PubMed, Embase, and Web of Science. We included studies on objectively quantified differential gene expression and/or protein composition in human rotator cuff tendinopathy and other tendinopathies as compared to control tissue.

Results: We identified 2199 studies, of which 54 were included; 25 studies focussed on rotator cuff or biceps tendinopathy. Most of the included studies quantified prespecified mRNA molecules and proteins using polymerase chain reactions and immunoassays, respectively. There was a tendency towards an increase of collagen I (11 of 15 studies) and III (13 of 14), metalloproteinase (MMP)-1 (6 of 12), -9 (7 of 7), -13 (4 of 7), tissue inhibitor of metalloproteinase (TIMP)-1 (4 of 7), and vascular endothelial growth factor (4 of 7), and a decrease in MMP-3 (10 of 12). Fourteen proteomics studies of tendon tissues/cells failed inclusion, mostly because they were conducted in animals or in vitro.

Conclusions: Based on methods, which only allowed simultaneous quantification of a limited number of prespecified mRNA molecules or proteins, several proteins appeared to be differentially expressed/represented in rotator cuff tendinopathy and other tendinopathies. No proteomics studies fulfilled our inclusion criteria, although proteomics technologies may be a way to identify protein profiles (including non-prespecified proteins) that characterise specific tendon disorders or stages of tendinopathy. Thus, our results suggested an untapped potential for proteomics in tendon research.

Show MeSH
Related in: MedlinePlus