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A Comparative Study of Iron Uptake Rates and Mechanisms amongst Marine and Fresh Water Cyanobacteria: Prevalence of Reductive Iron Uptake.

Lis H, Kranzler C, Keren N, Shaked Y - Life (Basel) (2015)

Bottom Line: Uniformity in kin/SA suggests similarity in the mechanism of uptake and indeed, all strains were found to employ a reductive step in the uptake of Fe' and FOB.In contrast, different uptake pathways were found for FeAB along with variations in kin/SA.Cyanobacteria combining both uptake strategies benefit from increased flexibility in accessing different Fe-substrates.

View Article: PubMed Central - PubMed

Affiliation: Interuniversity Institute for Marine Sciences in Eilat, Israel. hagarlis@gmail.com.

ABSTRACT
In this contribution, we address the question of iron bioavailability to cyanobacteria by measuring Fe uptake rates and probing for a reductive uptake pathway in diverse cyanobacterial species. We examined three Fe-substrates: dissolved inorganic iron (Fe') and the Fe-siderophores Ferrioxamine B (FOB) and FeAerobactin (FeAB). In order to compare across substrates and strains, we extracted uptake rate constants (kin = uptake rate/[Fe-substrate]). Fe' was the most bioavailable Fe form to cyanobacteria, with kin values higher than those of other substrates. When accounting for surface area (SA), all strains acquired Fe' at similar rates, as their kin/SA were similar. We also observed homogeneity in the uptake of FOB among strains, but with 10,000 times lower kin/SA values than Fe'. Uniformity in kin/SA suggests similarity in the mechanism of uptake and indeed, all strains were found to employ a reductive step in the uptake of Fe' and FOB. In contrast, different uptake pathways were found for FeAB along with variations in kin/SA. Our data supports the existence of a common reductive Fe uptake pathway amongst cyanobacteria, functioning alone or in addition to siderophore-mediated uptake. Cyanobacteria combining both uptake strategies benefit from increased flexibility in accessing different Fe-substrates.

No MeSH data available.


55FeAerobactin (FeAB) uptake as compared to Fe' and FOB uptake by five representative strains of iron limited cyanobacteria. In order to compare between species, uptake rate constants (kin) were normalized to cell surface area (i.e., kin/S.A in units of L·μm−2·h−1).
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life-05-00841-f006: 55FeAerobactin (FeAB) uptake as compared to Fe' and FOB uptake by five representative strains of iron limited cyanobacteria. In order to compare between species, uptake rate constants (kin) were normalized to cell surface area (i.e., kin/S.A in units of L·μm−2·h−1).

Mentions: Comparison of FeAB uptake across organisms can be made after normalization of the uptake rate constants to cell surface area (kin/S.A)—see Figure 6. Relative to Fe' and FOB uptake, three distinct behaviors can be seen in FeAB uptake: (1) No uptake amongst marine strains; (2) more efficient uptake than FOB in Synechocystis PCC6803, and (3) FeAB which is similar to or lower than FOB uptake in Synechococcus PCC7002 and Anabaena UTEX2576.


A Comparative Study of Iron Uptake Rates and Mechanisms amongst Marine and Fresh Water Cyanobacteria: Prevalence of Reductive Iron Uptake.

Lis H, Kranzler C, Keren N, Shaked Y - Life (Basel) (2015)

55FeAerobactin (FeAB) uptake as compared to Fe' and FOB uptake by five representative strains of iron limited cyanobacteria. In order to compare between species, uptake rate constants (kin) were normalized to cell surface area (i.e., kin/S.A in units of L·μm−2·h−1).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390881&req=5

life-05-00841-f006: 55FeAerobactin (FeAB) uptake as compared to Fe' and FOB uptake by five representative strains of iron limited cyanobacteria. In order to compare between species, uptake rate constants (kin) were normalized to cell surface area (i.e., kin/S.A in units of L·μm−2·h−1).
Mentions: Comparison of FeAB uptake across organisms can be made after normalization of the uptake rate constants to cell surface area (kin/S.A)—see Figure 6. Relative to Fe' and FOB uptake, three distinct behaviors can be seen in FeAB uptake: (1) No uptake amongst marine strains; (2) more efficient uptake than FOB in Synechocystis PCC6803, and (3) FeAB which is similar to or lower than FOB uptake in Synechococcus PCC7002 and Anabaena UTEX2576.

Bottom Line: Uniformity in kin/SA suggests similarity in the mechanism of uptake and indeed, all strains were found to employ a reductive step in the uptake of Fe' and FOB.In contrast, different uptake pathways were found for FeAB along with variations in kin/SA.Cyanobacteria combining both uptake strategies benefit from increased flexibility in accessing different Fe-substrates.

View Article: PubMed Central - PubMed

Affiliation: Interuniversity Institute for Marine Sciences in Eilat, Israel. hagarlis@gmail.com.

ABSTRACT
In this contribution, we address the question of iron bioavailability to cyanobacteria by measuring Fe uptake rates and probing for a reductive uptake pathway in diverse cyanobacterial species. We examined three Fe-substrates: dissolved inorganic iron (Fe') and the Fe-siderophores Ferrioxamine B (FOB) and FeAerobactin (FeAB). In order to compare across substrates and strains, we extracted uptake rate constants (kin = uptake rate/[Fe-substrate]). Fe' was the most bioavailable Fe form to cyanobacteria, with kin values higher than those of other substrates. When accounting for surface area (SA), all strains acquired Fe' at similar rates, as their kin/SA were similar. We also observed homogeneity in the uptake of FOB among strains, but with 10,000 times lower kin/SA values than Fe'. Uniformity in kin/SA suggests similarity in the mechanism of uptake and indeed, all strains were found to employ a reductive step in the uptake of Fe' and FOB. In contrast, different uptake pathways were found for FeAB along with variations in kin/SA. Our data supports the existence of a common reductive Fe uptake pathway amongst cyanobacteria, functioning alone or in addition to siderophore-mediated uptake. Cyanobacteria combining both uptake strategies benefit from increased flexibility in accessing different Fe-substrates.

No MeSH data available.