Limits...
From vesicles to protocells: the roles of amphiphilic molecules.

Sakuma Y, Imai M - Life (Basel) (2015)

Bottom Line: It is very challenging to construct protocells from molecular assemblies.Here, we show that simple binary phospholipid vesicles have the potential to reproduce the relevant functions of adhesion, pore formation and self-reproduction of vesicles, by coupling the lipid geometries (spontaneous curvatures) and the phase separation.This achievement will elucidate the pathway from molecular assembly to cellular life.

View Article: PubMed Central - PubMed

Affiliation: Department of Physics, Tohoku University, Aoba, Sendai 980-8578, Japan. sakuma@bio.phys.tohoku.ac.jp.

ABSTRACT
It is very challenging to construct protocells from molecular assemblies. An important step in this challenge is the achievement of vesicle dynamics that are relevant to cellular functions, such as membrane trafficking and self-reproduction, using amphiphilic molecules. Soft matter physics will play an important role in the development of vesicles that have these functions. Here, we show that simple binary phospholipid vesicles have the potential to reproduce the relevant functions of adhesion, pore formation and self-reproduction of vesicles, by coupling the lipid geometries (spontaneous curvatures) and the phase separation. This achievement will elucidate the pathway from molecular assembly to cellular life.

No MeSH data available.


Related in: MedlinePlus

Fluorescence images of (a) adhering DPPE/DOPC binary GUVs and (b) 1,2-diphytanoyl-sn-glycero-3-phosphocholine (DPhPC)/DPPC binary GUVs. Scale bars indicate 10 μm (taken from [48]).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4390873&req=5

life-05-00651-f014: Fluorescence images of (a) adhering DPPE/DOPC binary GUVs and (b) 1,2-diphytanoyl-sn-glycero-3-phosphocholine (DPhPC)/DPPC binary GUVs. Scale bars indicate 10 μm (taken from [48]).

Mentions: Figure 14 shows images of adhering binary GUV: (a) DPPE/DOPC and (b) DPhPC/DPPC. The GUVs were dyed with Texas red-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (TR-DHPE), and TR-DHPE is localized in the liquid phases. For DPPE/DOPC binary GUVs, the GUVs adhere to one another through the dark region, namely the DPPE-rich domains. Similarly, the adhering region of DPhPC/DPPC binary GUVs shows an intense line, indicating that the adhesion takes place through the DPhPC-rich region. These images clearly show that the GUVs adhere to one another through the domains that are rich in the inverse-cone-shaped lipids.


From vesicles to protocells: the roles of amphiphilic molecules.

Sakuma Y, Imai M - Life (Basel) (2015)

Fluorescence images of (a) adhering DPPE/DOPC binary GUVs and (b) 1,2-diphytanoyl-sn-glycero-3-phosphocholine (DPhPC)/DPPC binary GUVs. Scale bars indicate 10 μm (taken from [48]).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390873&req=5

life-05-00651-f014: Fluorescence images of (a) adhering DPPE/DOPC binary GUVs and (b) 1,2-diphytanoyl-sn-glycero-3-phosphocholine (DPhPC)/DPPC binary GUVs. Scale bars indicate 10 μm (taken from [48]).
Mentions: Figure 14 shows images of adhering binary GUV: (a) DPPE/DOPC and (b) DPhPC/DPPC. The GUVs were dyed with Texas red-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (TR-DHPE), and TR-DHPE is localized in the liquid phases. For DPPE/DOPC binary GUVs, the GUVs adhere to one another through the dark region, namely the DPPE-rich domains. Similarly, the adhering region of DPhPC/DPPC binary GUVs shows an intense line, indicating that the adhesion takes place through the DPhPC-rich region. These images clearly show that the GUVs adhere to one another through the domains that are rich in the inverse-cone-shaped lipids.

Bottom Line: It is very challenging to construct protocells from molecular assemblies.Here, we show that simple binary phospholipid vesicles have the potential to reproduce the relevant functions of adhesion, pore formation and self-reproduction of vesicles, by coupling the lipid geometries (spontaneous curvatures) and the phase separation.This achievement will elucidate the pathway from molecular assembly to cellular life.

View Article: PubMed Central - PubMed

Affiliation: Department of Physics, Tohoku University, Aoba, Sendai 980-8578, Japan. sakuma@bio.phys.tohoku.ac.jp.

ABSTRACT
It is very challenging to construct protocells from molecular assemblies. An important step in this challenge is the achievement of vesicle dynamics that are relevant to cellular functions, such as membrane trafficking and self-reproduction, using amphiphilic molecules. Soft matter physics will play an important role in the development of vesicles that have these functions. Here, we show that simple binary phospholipid vesicles have the potential to reproduce the relevant functions of adhesion, pore formation and self-reproduction of vesicles, by coupling the lipid geometries (spontaneous curvatures) and the phase separation. This achievement will elucidate the pathway from molecular assembly to cellular life.

No MeSH data available.


Related in: MedlinePlus