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Regulation of Genes Involved in Heterocyst Differentiation in the Cyanobacterium Anabaena sp. Strain PCC 7120 by a Group 2 Sigma Factor SigC.

Ehira S, Miyazaki S - Life (Basel) (2015)

Bottom Line: We carried out DNA microarray analysis to identify genes regulated by SigC, SigE, and SigG.It was indicated that SigC regulated the expression of genes involved in heterocyst differentiation and functions.Moreover, genes regulated by SigC partially overlapped with those regulated by SigE, and deficiency of SigC was likely to be compensated by SigE.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Graduate School of Science and Engineering, Tokyo Metropolitan University, Hachioji, Tokyo 192-0397, Japan. ehira@tmu.ac.jp.

ABSTRACT
The filamentous cyanobacterium Anabaena sp. strain PCC 7120 differentiates specialized cells for nitrogen fixation called heterocysts upon limitation of combined nitrogen in the medium. During heterocyst differentiation, expression of approximately 500 genes is upregulated with spatiotemporal regulation. In the present study, we investigated the functions of sigma factors of RNA polymerase in the regulation of heterocyst differentiation. The transcript levels of sigC, sigE, and sigG were increased during heterocyst differentiation, while expression of sigJ was downregulated. We carried out DNA microarray analysis to identify genes regulated by SigC, SigE, and SigG. It was indicated that SigC regulated the expression of genes involved in heterocyst differentiation and functions. Moreover, genes regulated by SigC partially overlapped with those regulated by SigE, and deficiency of SigC was likely to be compensated by SigE.

No MeSH data available.


Related in: MedlinePlus

Changes in the transcript levels of genes downregulated by sigC disruption during heterocyst differentiation. The transcript levels of fbp, talB, coxB3, coxA2, hepA, hepB, all4160, and ntcA were determined in the wild-type (WT) strain (white bars) and the sigC disruptant (gray bars). The transcript levels at 0 h in the WT strain were set to 1. The t-test was used to compare the WT strain and the sigC disruptant, and data that represent a significant difference (p < 0.01 or 0.05) are marked with black or white circles.
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life-05-00587-f003: Changes in the transcript levels of genes downregulated by sigC disruption during heterocyst differentiation. The transcript levels of fbp, talB, coxB3, coxA2, hepA, hepB, all4160, and ntcA were determined in the wild-type (WT) strain (white bars) and the sigC disruptant (gray bars). The transcript levels at 0 h in the WT strain were set to 1. The t-test was used to compare the WT strain and the sigC disruptant, and data that represent a significant difference (p < 0.01 or 0.05) are marked with black or white circles.

Mentions: We further analyzed the effects of sigC inactivation on gene expression during a time series of 24 h of heterocyst differentiation by qRT-PCR. The transcript levels of fbp, talB, coxB3, coxA2, hepA, hepB, all4160, and ntcA in DRsigCS after 8 h of nitrogen deprivation were lower than those in the WT strain (Figure 3). In the WT strain, expression of all these genes, except for that of coxB3, was constant or decreased between 8 h and 24 h, while their expression in DRsigCS was increased, which could be due to transcription by RNA polymerase with a sigma factor other than SigC. It is indicated that SigC is required for upregulation of genes expressed in heterocysts, although another sigma factor(s) can compensate for the deficiency of SigC.


Regulation of Genes Involved in Heterocyst Differentiation in the Cyanobacterium Anabaena sp. Strain PCC 7120 by a Group 2 Sigma Factor SigC.

Ehira S, Miyazaki S - Life (Basel) (2015)

Changes in the transcript levels of genes downregulated by sigC disruption during heterocyst differentiation. The transcript levels of fbp, talB, coxB3, coxA2, hepA, hepB, all4160, and ntcA were determined in the wild-type (WT) strain (white bars) and the sigC disruptant (gray bars). The transcript levels at 0 h in the WT strain were set to 1. The t-test was used to compare the WT strain and the sigC disruptant, and data that represent a significant difference (p < 0.01 or 0.05) are marked with black or white circles.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390870&req=5

life-05-00587-f003: Changes in the transcript levels of genes downregulated by sigC disruption during heterocyst differentiation. The transcript levels of fbp, talB, coxB3, coxA2, hepA, hepB, all4160, and ntcA were determined in the wild-type (WT) strain (white bars) and the sigC disruptant (gray bars). The transcript levels at 0 h in the WT strain were set to 1. The t-test was used to compare the WT strain and the sigC disruptant, and data that represent a significant difference (p < 0.01 or 0.05) are marked with black or white circles.
Mentions: We further analyzed the effects of sigC inactivation on gene expression during a time series of 24 h of heterocyst differentiation by qRT-PCR. The transcript levels of fbp, talB, coxB3, coxA2, hepA, hepB, all4160, and ntcA in DRsigCS after 8 h of nitrogen deprivation were lower than those in the WT strain (Figure 3). In the WT strain, expression of all these genes, except for that of coxB3, was constant or decreased between 8 h and 24 h, while their expression in DRsigCS was increased, which could be due to transcription by RNA polymerase with a sigma factor other than SigC. It is indicated that SigC is required for upregulation of genes expressed in heterocysts, although another sigma factor(s) can compensate for the deficiency of SigC.

Bottom Line: We carried out DNA microarray analysis to identify genes regulated by SigC, SigE, and SigG.It was indicated that SigC regulated the expression of genes involved in heterocyst differentiation and functions.Moreover, genes regulated by SigC partially overlapped with those regulated by SigE, and deficiency of SigC was likely to be compensated by SigE.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Graduate School of Science and Engineering, Tokyo Metropolitan University, Hachioji, Tokyo 192-0397, Japan. ehira@tmu.ac.jp.

ABSTRACT
The filamentous cyanobacterium Anabaena sp. strain PCC 7120 differentiates specialized cells for nitrogen fixation called heterocysts upon limitation of combined nitrogen in the medium. During heterocyst differentiation, expression of approximately 500 genes is upregulated with spatiotemporal regulation. In the present study, we investigated the functions of sigma factors of RNA polymerase in the regulation of heterocyst differentiation. The transcript levels of sigC, sigE, and sigG were increased during heterocyst differentiation, while expression of sigJ was downregulated. We carried out DNA microarray analysis to identify genes regulated by SigC, SigE, and SigG. It was indicated that SigC regulated the expression of genes involved in heterocyst differentiation and functions. Moreover, genes regulated by SigC partially overlapped with those regulated by SigE, and deficiency of SigC was likely to be compensated by SigE.

No MeSH data available.


Related in: MedlinePlus