Limits...
Modes of Fatty Acid desaturation in cyanobacteria: an update.

Los DA, Mironov KS - Life (Basel) (2015)

Bottom Line: Today, new genomic data are available, which support a need to update a previously postulated FA-based classification of cyanobacteria.These changes are necessary in order to adjust and synchronize biochemical, physiological and genomic data, which may help to establish an adequate comprehensive taxonomic system for cyanobacteria in the future.Here, we propose an update to the classification system of cyanobacteria based on their fatty acid composition.

View Article: PubMed Central - PubMed

Affiliation: Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street, Moscow 127276, Russia. losda@ippras.ru.

ABSTRACT
Fatty acid composition of individual species of cyanobacteria is conserved and it may be used as a phylogenetic marker. The previously proposed classification system was based solely on biochemical data. Today, new genomic data are available, which support a need to update a previously postulated FA-based classification of cyanobacteria. These changes are necessary in order to adjust and synchronize biochemical, physiological and genomic data, which may help to establish an adequate comprehensive taxonomic system for cyanobacteria in the future. Here, we propose an update to the classification system of cyanobacteria based on their fatty acid composition.

No MeSH data available.


Alignment of partial amino acid sequences of the acyl-lipid fatty acid Δ9-desaturases from different cyanobacteria. The desaturases are clustered into three types of enzymes, DesC1, DesC2, and DesC3, according to their amino acid and functional features. Four conservative histidine-containing domains are marked. Amino acids identical or similar in all three groups of the Δ9-desaturases are shown in green; amino acids identical in two groups of desaturases are shown in blue; amino acids, which are unique for one of the desaturase groups, are shown in orange.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4390868&req=5

life-05-00554-f001: Alignment of partial amino acid sequences of the acyl-lipid fatty acid Δ9-desaturases from different cyanobacteria. The desaturases are clustered into three types of enzymes, DesC1, DesC2, and DesC3, according to their amino acid and functional features. Four conservative histidine-containing domains are marked. Amino acids identical or similar in all three groups of the Δ9-desaturases are shown in green; amino acids identical in two groups of desaturases are shown in blue; amino acids, which are unique for one of the desaturase groups, are shown in orange.

Mentions: The alignment of amino acid sequences of Δ9-desaturases from various strains of cyanobacteria revealed that these enzymes can be classified into three groups (Figure 1). The first group, DesC1, is represented by the enzymes that are similar to the Δ9-desaturase, which is specific to sn-1 position of glycerolipids in Synechocystis sp. PCC 6803 and Anabaena variabilis [25]. Second group, DesC2, forms a cluster of enzymes homologous to the Δ9-desaturase, which is specific to sn-2 position in Antarctic Nostoc sp. 36 [21]. Differences in specificity of DesC1 and DesC2 to sn-position were demonstrated in accurate biochemical experiments [21,25]. The third distinct group of Δ9-desaturases, DesC3, is clustered by four amino acid sequences that were deduced from the genomic data of Gloeobacter violaceus [24] and two sequences of other cyanobacterial species.


Modes of Fatty Acid desaturation in cyanobacteria: an update.

Los DA, Mironov KS - Life (Basel) (2015)

Alignment of partial amino acid sequences of the acyl-lipid fatty acid Δ9-desaturases from different cyanobacteria. The desaturases are clustered into three types of enzymes, DesC1, DesC2, and DesC3, according to their amino acid and functional features. Four conservative histidine-containing domains are marked. Amino acids identical or similar in all three groups of the Δ9-desaturases are shown in green; amino acids identical in two groups of desaturases are shown in blue; amino acids, which are unique for one of the desaturase groups, are shown in orange.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390868&req=5

life-05-00554-f001: Alignment of partial amino acid sequences of the acyl-lipid fatty acid Δ9-desaturases from different cyanobacteria. The desaturases are clustered into three types of enzymes, DesC1, DesC2, and DesC3, according to their amino acid and functional features. Four conservative histidine-containing domains are marked. Amino acids identical or similar in all three groups of the Δ9-desaturases are shown in green; amino acids identical in two groups of desaturases are shown in blue; amino acids, which are unique for one of the desaturase groups, are shown in orange.
Mentions: The alignment of amino acid sequences of Δ9-desaturases from various strains of cyanobacteria revealed that these enzymes can be classified into three groups (Figure 1). The first group, DesC1, is represented by the enzymes that are similar to the Δ9-desaturase, which is specific to sn-1 position of glycerolipids in Synechocystis sp. PCC 6803 and Anabaena variabilis [25]. Second group, DesC2, forms a cluster of enzymes homologous to the Δ9-desaturase, which is specific to sn-2 position in Antarctic Nostoc sp. 36 [21]. Differences in specificity of DesC1 and DesC2 to sn-position were demonstrated in accurate biochemical experiments [21,25]. The third distinct group of Δ9-desaturases, DesC3, is clustered by four amino acid sequences that were deduced from the genomic data of Gloeobacter violaceus [24] and two sequences of other cyanobacterial species.

Bottom Line: Today, new genomic data are available, which support a need to update a previously postulated FA-based classification of cyanobacteria.These changes are necessary in order to adjust and synchronize biochemical, physiological and genomic data, which may help to establish an adequate comprehensive taxonomic system for cyanobacteria in the future.Here, we propose an update to the classification system of cyanobacteria based on their fatty acid composition.

View Article: PubMed Central - PubMed

Affiliation: Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street, Moscow 127276, Russia. losda@ippras.ru.

ABSTRACT
Fatty acid composition of individual species of cyanobacteria is conserved and it may be used as a phylogenetic marker. The previously proposed classification system was based solely on biochemical data. Today, new genomic data are available, which support a need to update a previously postulated FA-based classification of cyanobacteria. These changes are necessary in order to adjust and synchronize biochemical, physiological and genomic data, which may help to establish an adequate comprehensive taxonomic system for cyanobacteria in the future. Here, we propose an update to the classification system of cyanobacteria based on their fatty acid composition.

No MeSH data available.