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pTC Plasmids from Sulfolobus Species in the Geothermal Area of Tengchong, China: Genomic Conservation and Naturally-Occurring Variations as a Result of Transposition by Mobile Genetic Elements.

Xiang X, Huang X, Wang H, Huang L - Life (Basel) (2015)

Bottom Line: However, attempts to demonstrate experimentally the capacity of the plasmid for conjugational transfer were unsuccessful.The IS was efficiently inserted into the pTC1 genome, and the inserted sequence was inactivated and degraded more frequently in an imprecise manner than in a precise manner.These results suggest that the host organism has evolved a strategy to maintain a balance between the insertion and elimination of mobile genetic elements to permit genomic plasticity while inhibiting their fast spreading.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, No. 1 West Beichen Road, Chaoyang District, Beijing 100101, China. xiangxiaoyu@yahoo.com.

ABSTRACT
Plasmids occur frequently in Archaea. A novel plasmid (denoted pTC1) containing typical conjugation functions has been isolated from Sulfolobus tengchongensis RT8-4, a strain obtained from a hot spring in Tengchong, China, and characterized. The plasmid is a circular double-stranded DNA molecule of 20,417 bp. Among a total of 26 predicted pTC1 ORFs, 23 have homologues in other known Sulfolobus conjugative plasmids (CPs). pTC1 resembles other Sulfolobus CPs in genome architecture, and is most highly conserved in the genomic region encoding conjugation functions. However, attempts to demonstrate experimentally the capacity of the plasmid for conjugational transfer were unsuccessful. A survey revealed that pTC1 and its closely related plasmid variants were widespread in the geothermal area of Tengchong. Variations of the plasmids at the target sites for transposition by an insertion sequence (IS) and a miniature inverted-repeat transposable element (MITE) were readily detected. The IS was efficiently inserted into the pTC1 genome, and the inserted sequence was inactivated and degraded more frequently in an imprecise manner than in a precise manner. These results suggest that the host organism has evolved a strategy to maintain a balance between the insertion and elimination of mobile genetic elements to permit genomic plasticity while inhibiting their fast spreading.

No MeSH data available.


Related in: MedlinePlus

A map of pTC1. ORFs are shown by arrows. ORFs with no homologues in the other known Sulfolobus CPs are indicated with blank arrows, whereas those with homologues in other CPs are shown with black arrows. Functional sections A, B and C are indicated.
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life-05-00506-f002: A map of pTC1. ORFs are shown by arrows. ORFs with no homologues in the other known Sulfolobus CPs are indicated with blank arrows, whereas those with homologues in other CPs are shown with black arrows. Functional sections A, B and C are indicated.

Mentions: Both strands of plasmid pTC1 were sequenced. The restriction patterns of the plasmid, predicted from the genomic sequence, agreed well with the results of restriction digestion. As revealed by sequencing, pTC1 is a circular double-stranded DNA molecule of 20,417 bp in length. The G+C content of pTC1 was 41.4%, which was higher than that of its host (34.4%) [13] as well as those of other known Sulfolobus CPs. The plasmid contains 26 open reading frames (ORFs) (Figure 2; Table 1). In most of the plasmid, genes are tightly packed or even overlapping. Among all known Sulfolobus CPs, pTC1 is the smallest and carries fewest ORFs.


pTC Plasmids from Sulfolobus Species in the Geothermal Area of Tengchong, China: Genomic Conservation and Naturally-Occurring Variations as a Result of Transposition by Mobile Genetic Elements.

Xiang X, Huang X, Wang H, Huang L - Life (Basel) (2015)

A map of pTC1. ORFs are shown by arrows. ORFs with no homologues in the other known Sulfolobus CPs are indicated with blank arrows, whereas those with homologues in other CPs are shown with black arrows. Functional sections A, B and C are indicated.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390865&req=5

life-05-00506-f002: A map of pTC1. ORFs are shown by arrows. ORFs with no homologues in the other known Sulfolobus CPs are indicated with blank arrows, whereas those with homologues in other CPs are shown with black arrows. Functional sections A, B and C are indicated.
Mentions: Both strands of plasmid pTC1 were sequenced. The restriction patterns of the plasmid, predicted from the genomic sequence, agreed well with the results of restriction digestion. As revealed by sequencing, pTC1 is a circular double-stranded DNA molecule of 20,417 bp in length. The G+C content of pTC1 was 41.4%, which was higher than that of its host (34.4%) [13] as well as those of other known Sulfolobus CPs. The plasmid contains 26 open reading frames (ORFs) (Figure 2; Table 1). In most of the plasmid, genes are tightly packed or even overlapping. Among all known Sulfolobus CPs, pTC1 is the smallest and carries fewest ORFs.

Bottom Line: However, attempts to demonstrate experimentally the capacity of the plasmid for conjugational transfer were unsuccessful.The IS was efficiently inserted into the pTC1 genome, and the inserted sequence was inactivated and degraded more frequently in an imprecise manner than in a precise manner.These results suggest that the host organism has evolved a strategy to maintain a balance between the insertion and elimination of mobile genetic elements to permit genomic plasticity while inhibiting their fast spreading.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, No. 1 West Beichen Road, Chaoyang District, Beijing 100101, China. xiangxiaoyu@yahoo.com.

ABSTRACT
Plasmids occur frequently in Archaea. A novel plasmid (denoted pTC1) containing typical conjugation functions has been isolated from Sulfolobus tengchongensis RT8-4, a strain obtained from a hot spring in Tengchong, China, and characterized. The plasmid is a circular double-stranded DNA molecule of 20,417 bp. Among a total of 26 predicted pTC1 ORFs, 23 have homologues in other known Sulfolobus conjugative plasmids (CPs). pTC1 resembles other Sulfolobus CPs in genome architecture, and is most highly conserved in the genomic region encoding conjugation functions. However, attempts to demonstrate experimentally the capacity of the plasmid for conjugational transfer were unsuccessful. A survey revealed that pTC1 and its closely related plasmid variants were widespread in the geothermal area of Tengchong. Variations of the plasmids at the target sites for transposition by an insertion sequence (IS) and a miniature inverted-repeat transposable element (MITE) were readily detected. The IS was efficiently inserted into the pTC1 genome, and the inserted sequence was inactivated and degraded more frequently in an imprecise manner than in a precise manner. These results suggest that the host organism has evolved a strategy to maintain a balance between the insertion and elimination of mobile genetic elements to permit genomic plasticity while inhibiting their fast spreading.

No MeSH data available.


Related in: MedlinePlus