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Does DNA exert an active role in generating cell-sized spheres in an aqueous solution with a crowding binary polymer?

Tsumoto K, Arai M, Nakatani N, Watanabe SN, Yoshikawa K - Life (Basel) (2015)

Bottom Line: DNA molecules were selectively located in the interior of dextran-rich micro-droplets, when the composition of an aqueous two-phase system (ATPS) was near the critical condition of phase-segregation.The resulting micro-droplets could be controlled by the use of optical tweezers.A hypothetical scenario for the emergence of a primitive cell with DNA is briefly discussed.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Engineering, Mie University, Mie, 514-8507, Japan. tsumoto@chem.mie-u.ac.jp.

ABSTRACT
We report the spontaneous generation of a cell-like morphology in an environment crowded with the polymers dextran and polyethylene glycol (PEG) in the presence of DNA. DNA molecules were selectively located in the interior of dextran-rich micro-droplets, when the composition of an aqueous two-phase system (ATPS) was near the critical condition of phase-segregation. The resulting micro-droplets could be controlled by the use of optical tweezers. As an example of laser manipulation, the dynamic fusion of two droplets is reported, which resembles the process of cell division in time-reverse. A hypothetical scenario for the emergence of a primitive cell with DNA is briefly discussed.

No MeSH data available.


Trapping, transport and fusion of microspheres in an ATPS consisting of dextran and PEG. (a) The upper phase contained dextran-rich microspheres in a PEG-rich exterior solution. (b) The lower phase contained PEG-rich microspheres in a dextran-rich exterior solution. In both cases, microspheres were successfully fused. Times after trapping are indicated. The focus point of the laser is marked by crosses as a guide. Dextran and PEG are 1.5% and 7% PEG, respectively. Bar: 10 µm.
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life-05-00459-f003: Trapping, transport and fusion of microspheres in an ATPS consisting of dextran and PEG. (a) The upper phase contained dextran-rich microspheres in a PEG-rich exterior solution. (b) The lower phase contained PEG-rich microspheres in a dextran-rich exterior solution. In both cases, microspheres were successfully fused. Times after trapping are indicated. The focus point of the laser is marked by crosses as a guide. Dextran and PEG are 1.5% and 7% PEG, respectively. Bar: 10 µm.

Mentions: As has been reported for some decades, macromolecular assemblies and aggregates such as giant DNA condensates [14], giant unilamellar liposomes [15], etc., can be directly trapped and manipulated using optical tweezers. In the present study, we verified whether microspheres could be trapped in the ATPS using a laser. Figure 3 shows that dextran- or PEG-rich microspheres with diameters larger than 10 µm were transferred, attached to other spheres, and then fused to become larger spheres.


Does DNA exert an active role in generating cell-sized spheres in an aqueous solution with a crowding binary polymer?

Tsumoto K, Arai M, Nakatani N, Watanabe SN, Yoshikawa K - Life (Basel) (2015)

Trapping, transport and fusion of microspheres in an ATPS consisting of dextran and PEG. (a) The upper phase contained dextran-rich microspheres in a PEG-rich exterior solution. (b) The lower phase contained PEG-rich microspheres in a dextran-rich exterior solution. In both cases, microspheres were successfully fused. Times after trapping are indicated. The focus point of the laser is marked by crosses as a guide. Dextran and PEG are 1.5% and 7% PEG, respectively. Bar: 10 µm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390863&req=5

life-05-00459-f003: Trapping, transport and fusion of microspheres in an ATPS consisting of dextran and PEG. (a) The upper phase contained dextran-rich microspheres in a PEG-rich exterior solution. (b) The lower phase contained PEG-rich microspheres in a dextran-rich exterior solution. In both cases, microspheres were successfully fused. Times after trapping are indicated. The focus point of the laser is marked by crosses as a guide. Dextran and PEG are 1.5% and 7% PEG, respectively. Bar: 10 µm.
Mentions: As has been reported for some decades, macromolecular assemblies and aggregates such as giant DNA condensates [14], giant unilamellar liposomes [15], etc., can be directly trapped and manipulated using optical tweezers. In the present study, we verified whether microspheres could be trapped in the ATPS using a laser. Figure 3 shows that dextran- or PEG-rich microspheres with diameters larger than 10 µm were transferred, attached to other spheres, and then fused to become larger spheres.

Bottom Line: DNA molecules were selectively located in the interior of dextran-rich micro-droplets, when the composition of an aqueous two-phase system (ATPS) was near the critical condition of phase-segregation.The resulting micro-droplets could be controlled by the use of optical tweezers.A hypothetical scenario for the emergence of a primitive cell with DNA is briefly discussed.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Engineering, Mie University, Mie, 514-8507, Japan. tsumoto@chem.mie-u.ac.jp.

ABSTRACT
We report the spontaneous generation of a cell-like morphology in an environment crowded with the polymers dextran and polyethylene glycol (PEG) in the presence of DNA. DNA molecules were selectively located in the interior of dextran-rich micro-droplets, when the composition of an aqueous two-phase system (ATPS) was near the critical condition of phase-segregation. The resulting micro-droplets could be controlled by the use of optical tweezers. As an example of laser manipulation, the dynamic fusion of two droplets is reported, which resembles the process of cell division in time-reverse. A hypothetical scenario for the emergence of a primitive cell with DNA is briefly discussed.

No MeSH data available.