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The Anabaena sp. PCC 7120 Exoproteome: Taking a Peek outside the Box.

Oliveira P, Martins NM, Santos M, Couto NA, Wright PC, Tamagnini P - Life (Basel) (2015)

Bottom Line: The evidence presented here shows that Anabaena sp.Furthermore, the activity of selected exoproteins associated with oxidative stress has been assessed, suggesting their involvement in redox homeostasis mechanisms in the extracellular space.Finally, we discuss our results in light of other cyanobacterial exoproteome studies and focus on the potential of exploring cyanobacteria as cell factories to produce and secrete selected proteins.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto 4150-180, Portugal. paulo.oliveira@ibmc.up.pt.

ABSTRACT
The interest in examining the subset of proteins present in the extracellular milieu, the exoproteome, has been growing due to novel insights highlighting their role on extracellular matrix organization and biofilm formation, but also on homeostasis and development. The cyanobacterial exoproteome is poorly studied, and the role of cyanobacterial exoproteins on cell wall biogenesis, morphology and even physiology is largely unknown. Here, we present a comprehensive examination of the Anabaena sp. PCC 7120 exoproteome under various growth conditions. Altogether, 139 proteins belonging to 16 different functional categories have been identified. A large fraction (48%) of the identified proteins is classified as "hypothetical", falls into the "other categories" set or presents no similarity to other proteins. The evidence presented here shows that Anabaena sp. PCC 7120 is capable of outer membrane vesicle formation and that these vesicles are likely to contribute to the exoproteome profile. Furthermore, the activity of selected exoproteins associated with oxidative stress has been assessed, suggesting their involvement in redox homeostasis mechanisms in the extracellular space. Finally, we discuss our results in light of other cyanobacterial exoproteome studies and focus on the potential of exploring cyanobacteria as cell factories to produce and secrete selected proteins.

No MeSH data available.


Related in: MedlinePlus

Absolute frequency of the various functional categories (as ascribed by Cyanobase, http://genome.microbedb.jp/cyanobase) of the proteins identified in the exoproteome of Anabaena sp. PCC 7120.
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life-05-00130-f002: Absolute frequency of the various functional categories (as ascribed by Cyanobase, http://genome.microbedb.jp/cyanobase) of the proteins identified in the exoproteome of Anabaena sp. PCC 7120.

Mentions: A distribution analysis of the proteins listed in Table 1 based on their respective functional categories (Figure 2) reveals that approximately 48% of the identified exoproteome is classified as “conserved hypothetical” (20%), presents no similarity to other proteins (10%) or falls into the “other categories” set (18%). The latter group of proteins comprises members associated with several unrelated processes, such as “transposon-related functions” and “adaptation and atypical conditions”, or are simply categorized as “other”. Thus, this group includes proteins with completely different functions and that share no obvious metabolic connection. Overall, the high frequency (48%) of identified proteins belonging to categories of unknown function further exposes our lack of knowledge regarding the impact of exoproteins and respective extracellular processes on the cell structure and physiology.


The Anabaena sp. PCC 7120 Exoproteome: Taking a Peek outside the Box.

Oliveira P, Martins NM, Santos M, Couto NA, Wright PC, Tamagnini P - Life (Basel) (2015)

Absolute frequency of the various functional categories (as ascribed by Cyanobase, http://genome.microbedb.jp/cyanobase) of the proteins identified in the exoproteome of Anabaena sp. PCC 7120.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390845&req=5

life-05-00130-f002: Absolute frequency of the various functional categories (as ascribed by Cyanobase, http://genome.microbedb.jp/cyanobase) of the proteins identified in the exoproteome of Anabaena sp. PCC 7120.
Mentions: A distribution analysis of the proteins listed in Table 1 based on their respective functional categories (Figure 2) reveals that approximately 48% of the identified exoproteome is classified as “conserved hypothetical” (20%), presents no similarity to other proteins (10%) or falls into the “other categories” set (18%). The latter group of proteins comprises members associated with several unrelated processes, such as “transposon-related functions” and “adaptation and atypical conditions”, or are simply categorized as “other”. Thus, this group includes proteins with completely different functions and that share no obvious metabolic connection. Overall, the high frequency (48%) of identified proteins belonging to categories of unknown function further exposes our lack of knowledge regarding the impact of exoproteins and respective extracellular processes on the cell structure and physiology.

Bottom Line: The evidence presented here shows that Anabaena sp.Furthermore, the activity of selected exoproteins associated with oxidative stress has been assessed, suggesting their involvement in redox homeostasis mechanisms in the extracellular space.Finally, we discuss our results in light of other cyanobacterial exoproteome studies and focus on the potential of exploring cyanobacteria as cell factories to produce and secrete selected proteins.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto 4150-180, Portugal. paulo.oliveira@ibmc.up.pt.

ABSTRACT
The interest in examining the subset of proteins present in the extracellular milieu, the exoproteome, has been growing due to novel insights highlighting their role on extracellular matrix organization and biofilm formation, but also on homeostasis and development. The cyanobacterial exoproteome is poorly studied, and the role of cyanobacterial exoproteins on cell wall biogenesis, morphology and even physiology is largely unknown. Here, we present a comprehensive examination of the Anabaena sp. PCC 7120 exoproteome under various growth conditions. Altogether, 139 proteins belonging to 16 different functional categories have been identified. A large fraction (48%) of the identified proteins is classified as "hypothetical", falls into the "other categories" set or presents no similarity to other proteins. The evidence presented here shows that Anabaena sp. PCC 7120 is capable of outer membrane vesicle formation and that these vesicles are likely to contribute to the exoproteome profile. Furthermore, the activity of selected exoproteins associated with oxidative stress has been assessed, suggesting their involvement in redox homeostasis mechanisms in the extracellular space. Finally, we discuss our results in light of other cyanobacterial exoproteome studies and focus on the potential of exploring cyanobacteria as cell factories to produce and secrete selected proteins.

No MeSH data available.


Related in: MedlinePlus