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Occurrence of Far-Red Light Photoacclimation (FaRLiP) in Diverse Cyanobacteria.

Gan F, Shen G, Bryant DA - Life (Basel) (2014)

Bottom Line: Leptolyngbya sp.JSC-1.We conclude that these photosynthetic gene clusters are diagnostic of the capacity to photoacclimate to and grow in far-red light.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA. fxg142@psu.edu.

ABSTRACT
Cyanobacteria have evolved a number of acclimation strategies to sense and respond to changing nutrient and light conditions. Leptolyngbya sp. JSC-1 was recently shown to photoacclimate to far-red light by extensively remodeling its photosystem (PS) I, PS II and phycobilisome complexes, thereby gaining the ability to grow in far-red light. A 21-gene photosynthetic gene cluster (rfpA/B/C, apcA2/B2/D2/E2/D3, psbA3/D3/C2/B2/ H2/A4, psaA2/B2/L2/I2/F2/J2) that is specifically expressed in far-red light encodes the core subunits of the three major photosynthetic complexes. The growth responses to far-red light were studied here for five additional cyanobacterial strains, each of which has a gene cluster similar to that in Leptolyngbya sp. JSC-1. After acclimation all five strains could grow continuously in far-red light. Under these growth conditions each strain synthesizes chlorophylls d, f and a after photoacclimation, and each strain produces modified forms of PS I, PS II (and phycobiliproteins) that absorb light between 700 and 800 nm. We conclude that these photosynthetic gene clusters are diagnostic of the capacity to photoacclimate to and grow in far-red light. Given the diversity of terrestrial environments from which these cyanobacteria were isolated, it is likely that FaRLiP plays an important role in optimizing photosynthesis in terrestrial environments.

No MeSH data available.


Related in: MedlinePlus

Comparison of whole-cell absorption spectra of cells grown in white light (WL; black lines) or far-red light (FRL; red lines). Each spectrum is the average of three measurements. To facilitate comparisons, the spectra were normalized at 680 nm. (A) Chr. thermalis PCC 7203; (B) Calothrix sp. PCC 7507; (C) F. thermalis PCC 7521; (D) Chlorogloeopsis sp. PCC 9212.
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life-05-00004-f003: Comparison of whole-cell absorption spectra of cells grown in white light (WL; black lines) or far-red light (FRL; red lines). Each spectrum is the average of three measurements. To facilitate comparisons, the spectra were normalized at 680 nm. (A) Chr. thermalis PCC 7203; (B) Calothrix sp. PCC 7507; (C) F. thermalis PCC 7521; (D) Chlorogloeopsis sp. PCC 9212.

Mentions: Four additional strains (Chr. thermalis PCC 7203, Calothrix sp. PCC 7507, F. thermalis PCC 7521, and Chlorogloeopsis sp. PCC 9212), all of which have photosynthetic gene clusters similar to that in Leptolyngbya JSC-1 (Figure 1), were also grown in FRL to determine if they too could perform FaRLiP. None of these four strains can synthesize phycoerythrin, and thus none of these strains is capable of CCA. However, biochemical studies [32] and genome analyses indicate that all four of these strains can synthesize phycoerythrocyanin. All four strains could be grown photoautotrophically in FRL. Figure 3 shows absorption spectra of whole cells of these strains after growth in WL or 720-nm FRL. In each case, the spectra show enhanced absorption beyond 700 nm in cells that had been grown in FRL, which indicates that new pigments and/or photosynthetic complexes had been synthesized.


Occurrence of Far-Red Light Photoacclimation (FaRLiP) in Diverse Cyanobacteria.

Gan F, Shen G, Bryant DA - Life (Basel) (2014)

Comparison of whole-cell absorption spectra of cells grown in white light (WL; black lines) or far-red light (FRL; red lines). Each spectrum is the average of three measurements. To facilitate comparisons, the spectra were normalized at 680 nm. (A) Chr. thermalis PCC 7203; (B) Calothrix sp. PCC 7507; (C) F. thermalis PCC 7521; (D) Chlorogloeopsis sp. PCC 9212.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390838&req=5

life-05-00004-f003: Comparison of whole-cell absorption spectra of cells grown in white light (WL; black lines) or far-red light (FRL; red lines). Each spectrum is the average of three measurements. To facilitate comparisons, the spectra were normalized at 680 nm. (A) Chr. thermalis PCC 7203; (B) Calothrix sp. PCC 7507; (C) F. thermalis PCC 7521; (D) Chlorogloeopsis sp. PCC 9212.
Mentions: Four additional strains (Chr. thermalis PCC 7203, Calothrix sp. PCC 7507, F. thermalis PCC 7521, and Chlorogloeopsis sp. PCC 9212), all of which have photosynthetic gene clusters similar to that in Leptolyngbya JSC-1 (Figure 1), were also grown in FRL to determine if they too could perform FaRLiP. None of these four strains can synthesize phycoerythrin, and thus none of these strains is capable of CCA. However, biochemical studies [32] and genome analyses indicate that all four of these strains can synthesize phycoerythrocyanin. All four strains could be grown photoautotrophically in FRL. Figure 3 shows absorption spectra of whole cells of these strains after growth in WL or 720-nm FRL. In each case, the spectra show enhanced absorption beyond 700 nm in cells that had been grown in FRL, which indicates that new pigments and/or photosynthetic complexes had been synthesized.

Bottom Line: Leptolyngbya sp.JSC-1.We conclude that these photosynthetic gene clusters are diagnostic of the capacity to photoacclimate to and grow in far-red light.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA. fxg142@psu.edu.

ABSTRACT
Cyanobacteria have evolved a number of acclimation strategies to sense and respond to changing nutrient and light conditions. Leptolyngbya sp. JSC-1 was recently shown to photoacclimate to far-red light by extensively remodeling its photosystem (PS) I, PS II and phycobilisome complexes, thereby gaining the ability to grow in far-red light. A 21-gene photosynthetic gene cluster (rfpA/B/C, apcA2/B2/D2/E2/D3, psbA3/D3/C2/B2/ H2/A4, psaA2/B2/L2/I2/F2/J2) that is specifically expressed in far-red light encodes the core subunits of the three major photosynthetic complexes. The growth responses to far-red light were studied here for five additional cyanobacterial strains, each of which has a gene cluster similar to that in Leptolyngbya sp. JSC-1. After acclimation all five strains could grow continuously in far-red light. Under these growth conditions each strain synthesizes chlorophylls d, f and a after photoacclimation, and each strain produces modified forms of PS I, PS II (and phycobiliproteins) that absorb light between 700 and 800 nm. We conclude that these photosynthetic gene clusters are diagnostic of the capacity to photoacclimate to and grow in far-red light. Given the diversity of terrestrial environments from which these cyanobacteria were isolated, it is likely that FaRLiP plays an important role in optimizing photosynthesis in terrestrial environments.

No MeSH data available.


Related in: MedlinePlus