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VEGFR-1 Expression Relates to Fuhrman Nuclear Grade of Clear Cell Renal Cell Carcinoma.

Lkhagvadorj S, Oh SS, Lee MR, Jung JH, Chung HC, Cha SK, Eom M - J Lifestyle Med (2014)

Bottom Line: Therapeutic targeting directed against VEGF and VEGFR-2 has been proven to be successful for metastatic clear cell RCC (CCRCC).The results were compared with various clinicopathologic parameters of CCRCC and patients' survival.VEGFR-1 expression may be associated with favorable prognostic factors, particularly a lower Fuhrman nuclear grade in CCRCC.

View Article: PubMed Central - PubMed

Affiliation: Departments of Pathology, Yonsei University Wonju College of Medicine, Wonju, Korea.

ABSTRACT

Background: Increasing evidence suggests that vascular endothelial growth factor (VEGF) and VEGF receptor (VEGFR) 1 signaling may play an important role in the progression of pathological angiogenesis that occurs in many tumors, including renal cell carcinoma (RCC). Therapeutic targeting directed against VEGF and VEGFR-2 has been proven to be successful for metastatic clear cell RCC (CCRCC). However, the expression of VEGFR-1 and its association with prognostic parameters of CCRCC in the tumorigenesis of renal cancer remains unclear. Therefore, we examined the expression of VEGFR-1 and its prognostic significance in CCRCC.

Methods: Immunohistochemical staining for VEGFR-1 was performed on 126 formalin-fixed paraffin-embedded CCRCC tissue samples. Six of these cases were available for Western blot analyses. The results were compared with various clinicopathologic parameters of CCRCC and patients' survival.

Results: VEGFR-1 expression was detected in 59 cases (46.8%) of CCRCC. Higher VEGFR-1 expression was significantly correlated with a lower Fuhrman nuclear grade and the absence of renal pelvis invasion, although it was not related to patients' survival. Western blot analyses showed higher VEGFR-1 expression in low grade tumors.

Conclusion: VEGFR-1 expression may be associated with favorable prognostic factors, particularly a lower Fuhrman nuclear grade in CCRCC.

No MeSH data available.


Related in: MedlinePlus

Expression level of VEGFR-1 in CCRCC associated with tumor grade. (A) Representative immunoblotting of VEGFR-1. Expression level of VEGFR-1 in paired tissues of normal tissue (N) and tumor tissue (T), including low and high grades, were analyzed with immunoblotting. β-actin served as a protein loading control. (B) Relative expression of VEGFR-1 in non-tumor and tumor tissues. (mean ± SEM, n = 6). (C) Relative expression of VEGFR-1 in low and high grade tumor tissues. (mean ± SEM, n = 6).
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f2-jlm-04-64: Expression level of VEGFR-1 in CCRCC associated with tumor grade. (A) Representative immunoblotting of VEGFR-1. Expression level of VEGFR-1 in paired tissues of normal tissue (N) and tumor tissue (T), including low and high grades, were analyzed with immunoblotting. β-actin served as a protein loading control. (B) Relative expression of VEGFR-1 in non-tumor and tumor tissues. (mean ± SEM, n = 6). (C) Relative expression of VEGFR-1 in low and high grade tumor tissues. (mean ± SEM, n = 6).

Mentions: The IHC staining showed that VEGFR-1 was observed in the membrane and/or cytoplasm of the tumor cells (Fig. 1). In paired fresh tissues, VEGFR-1 was expressed in both CCRCC and normal renal parenchymal tissue (Fig. 2A). We found a significant reduction of VEGFR-1 in tumor tissue compared to non-tumor tissue (Fig. 2B). In addition, the expression level of VEGR-1 was significantly elevated in low grade tumors compared to high grade tumors (Fig. 2C).


VEGFR-1 Expression Relates to Fuhrman Nuclear Grade of Clear Cell Renal Cell Carcinoma.

Lkhagvadorj S, Oh SS, Lee MR, Jung JH, Chung HC, Cha SK, Eom M - J Lifestyle Med (2014)

Expression level of VEGFR-1 in CCRCC associated with tumor grade. (A) Representative immunoblotting of VEGFR-1. Expression level of VEGFR-1 in paired tissues of normal tissue (N) and tumor tissue (T), including low and high grades, were analyzed with immunoblotting. β-actin served as a protein loading control. (B) Relative expression of VEGFR-1 in non-tumor and tumor tissues. (mean ± SEM, n = 6). (C) Relative expression of VEGFR-1 in low and high grade tumor tissues. (mean ± SEM, n = 6).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390762&req=5

f2-jlm-04-64: Expression level of VEGFR-1 in CCRCC associated with tumor grade. (A) Representative immunoblotting of VEGFR-1. Expression level of VEGFR-1 in paired tissues of normal tissue (N) and tumor tissue (T), including low and high grades, were analyzed with immunoblotting. β-actin served as a protein loading control. (B) Relative expression of VEGFR-1 in non-tumor and tumor tissues. (mean ± SEM, n = 6). (C) Relative expression of VEGFR-1 in low and high grade tumor tissues. (mean ± SEM, n = 6).
Mentions: The IHC staining showed that VEGFR-1 was observed in the membrane and/or cytoplasm of the tumor cells (Fig. 1). In paired fresh tissues, VEGFR-1 was expressed in both CCRCC and normal renal parenchymal tissue (Fig. 2A). We found a significant reduction of VEGFR-1 in tumor tissue compared to non-tumor tissue (Fig. 2B). In addition, the expression level of VEGR-1 was significantly elevated in low grade tumors compared to high grade tumors (Fig. 2C).

Bottom Line: Therapeutic targeting directed against VEGF and VEGFR-2 has been proven to be successful for metastatic clear cell RCC (CCRCC).The results were compared with various clinicopathologic parameters of CCRCC and patients' survival.VEGFR-1 expression may be associated with favorable prognostic factors, particularly a lower Fuhrman nuclear grade in CCRCC.

View Article: PubMed Central - PubMed

Affiliation: Departments of Pathology, Yonsei University Wonju College of Medicine, Wonju, Korea.

ABSTRACT

Background: Increasing evidence suggests that vascular endothelial growth factor (VEGF) and VEGF receptor (VEGFR) 1 signaling may play an important role in the progression of pathological angiogenesis that occurs in many tumors, including renal cell carcinoma (RCC). Therapeutic targeting directed against VEGF and VEGFR-2 has been proven to be successful for metastatic clear cell RCC (CCRCC). However, the expression of VEGFR-1 and its association with prognostic parameters of CCRCC in the tumorigenesis of renal cancer remains unclear. Therefore, we examined the expression of VEGFR-1 and its prognostic significance in CCRCC.

Methods: Immunohistochemical staining for VEGFR-1 was performed on 126 formalin-fixed paraffin-embedded CCRCC tissue samples. Six of these cases were available for Western blot analyses. The results were compared with various clinicopathologic parameters of CCRCC and patients' survival.

Results: VEGFR-1 expression was detected in 59 cases (46.8%) of CCRCC. Higher VEGFR-1 expression was significantly correlated with a lower Fuhrman nuclear grade and the absence of renal pelvis invasion, although it was not related to patients' survival. Western blot analyses showed higher VEGFR-1 expression in low grade tumors.

Conclusion: VEGFR-1 expression may be associated with favorable prognostic factors, particularly a lower Fuhrman nuclear grade in CCRCC.

No MeSH data available.


Related in: MedlinePlus