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Omentum Cells Promote Healing of Colonic Tissues in Dextran Sulfate Sodium (DSS) Induced Model of Colitis in Mice.

Jung BC, Lee MH, Sethupathi P, Lee IS, Lee D, Rhee KJ - J Lifestyle Med (2013)

Bottom Line: Inflammatory bowel disease is characterized by persistent inflammation of the intestinal tissues.Although the usage of biologics has greatly enhanced the management of this disorder, a permanent treatment does not exist.Thereafter, body weight change, serum KC levels, and histological analysis of the colon were conducted.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Korea.

ABSTRACT

Background: Inflammatory bowel disease is characterized by persistent inflammation of the intestinal tissues. Although the usage of biologics has greatly enhanced the management of this disorder, a permanent treatment does not exist. In this study, we investigated whether the cells with anti-inflammatory and healing properties from the omentum could be harnessed to treat colitis in dextran sulfate sodium (DSS)-induced mouse colitis model.

Methods: C57BL/6 mice were administered 2% DSS for 10 days and then injected in the peritoneum with cells isolated from the murine omentum. Thereafter, body weight change, serum KC levels, and histological analysis of the colon were conducted. We also examined if omentum infused mice were resistant to a lethal challenge of 4% DSS.

Results: 2% DSS-mice injected with omentum cells exhibited a decrease in body weight loss, decreased inflammation in the colon and decreased levels of the inflammatory cytokine KC in the serum compared to mice given 2% DSS alone. In addition, mice administered a lethal dose of 4% DSS exhibited a 50% decrease in mortality when injected with omentum cells.

Conclusion: Cells from the omentum exert anti-inflammatory and/or healing properties in the acute DSS-induced colitis model.

No MeSH data available.


Related in: MedlinePlus

Omentum cells decreases DSS-induced colitis. Mice were administered 2% DSS for 10 days and then injected intraperitoneally with omentum cells (2% DSS + omentum) or PBS (2% DSS). Representative H&E stained tissue sections are shown compared to mock treated mice (no DSS). The histologic inflammation scores of the colon were examined at day 21. *p-value <0.05 using the Mann-Whitney U test. Data are from 8 mice per group. Bars, 100 μm.
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f2-jlm-03-48: Omentum cells decreases DSS-induced colitis. Mice were administered 2% DSS for 10 days and then injected intraperitoneally with omentum cells (2% DSS + omentum) or PBS (2% DSS). Representative H&E stained tissue sections are shown compared to mock treated mice (no DSS). The histologic inflammation scores of the colon were examined at day 21. *p-value <0.05 using the Mann-Whitney U test. Data are from 8 mice per group. Bars, 100 μm.

Mentions: To determine if omentum protects against histological damage induced by DSS treatment, mice were administered 2% DSS for 10 days, injected with omentum cells and then euthanized 14 days later (day 24). Mice given 2% DSS alone for 10 days plus 14 days of water or untreated mice (i.e., no DSS) served as controls. We found that 2% DSS treated mice exhibited colonic inflammation throughout the colon with ulcerations observed in the distal region (Fig. 2). In addition, there was an increased frequency of colonic lymphoid follicles and extensive epithelial hyperplasia. In contrast, 2% DSS + omentum treated mice exhibited minimal ulcerations though they also showed extensive mucosal hyperplasia. Average colonic inflammation scores were significantly lower in the 2% DSS + omentum group compared to the 2% DSS group suggesting that omentum cells accelerated mucosa healings (Fig. 2). In addition, we found that KC levels (functional homolog of interleukin-8) in the serum were also decreased in DSS + omentum mice compared to DSS treated mice (Fig. 3).


Omentum Cells Promote Healing of Colonic Tissues in Dextran Sulfate Sodium (DSS) Induced Model of Colitis in Mice.

Jung BC, Lee MH, Sethupathi P, Lee IS, Lee D, Rhee KJ - J Lifestyle Med (2013)

Omentum cells decreases DSS-induced colitis. Mice were administered 2% DSS for 10 days and then injected intraperitoneally with omentum cells (2% DSS + omentum) or PBS (2% DSS). Representative H&E stained tissue sections are shown compared to mock treated mice (no DSS). The histologic inflammation scores of the colon were examined at day 21. *p-value <0.05 using the Mann-Whitney U test. Data are from 8 mice per group. Bars, 100 μm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390752&req=5

f2-jlm-03-48: Omentum cells decreases DSS-induced colitis. Mice were administered 2% DSS for 10 days and then injected intraperitoneally with omentum cells (2% DSS + omentum) or PBS (2% DSS). Representative H&E stained tissue sections are shown compared to mock treated mice (no DSS). The histologic inflammation scores of the colon were examined at day 21. *p-value <0.05 using the Mann-Whitney U test. Data are from 8 mice per group. Bars, 100 μm.
Mentions: To determine if omentum protects against histological damage induced by DSS treatment, mice were administered 2% DSS for 10 days, injected with omentum cells and then euthanized 14 days later (day 24). Mice given 2% DSS alone for 10 days plus 14 days of water or untreated mice (i.e., no DSS) served as controls. We found that 2% DSS treated mice exhibited colonic inflammation throughout the colon with ulcerations observed in the distal region (Fig. 2). In addition, there was an increased frequency of colonic lymphoid follicles and extensive epithelial hyperplasia. In contrast, 2% DSS + omentum treated mice exhibited minimal ulcerations though they also showed extensive mucosal hyperplasia. Average colonic inflammation scores were significantly lower in the 2% DSS + omentum group compared to the 2% DSS group suggesting that omentum cells accelerated mucosa healings (Fig. 2). In addition, we found that KC levels (functional homolog of interleukin-8) in the serum were also decreased in DSS + omentum mice compared to DSS treated mice (Fig. 3).

Bottom Line: Inflammatory bowel disease is characterized by persistent inflammation of the intestinal tissues.Although the usage of biologics has greatly enhanced the management of this disorder, a permanent treatment does not exist.Thereafter, body weight change, serum KC levels, and histological analysis of the colon were conducted.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Korea.

ABSTRACT

Background: Inflammatory bowel disease is characterized by persistent inflammation of the intestinal tissues. Although the usage of biologics has greatly enhanced the management of this disorder, a permanent treatment does not exist. In this study, we investigated whether the cells with anti-inflammatory and healing properties from the omentum could be harnessed to treat colitis in dextran sulfate sodium (DSS)-induced mouse colitis model.

Methods: C57BL/6 mice were administered 2% DSS for 10 days and then injected in the peritoneum with cells isolated from the murine omentum. Thereafter, body weight change, serum KC levels, and histological analysis of the colon were conducted. We also examined if omentum infused mice were resistant to a lethal challenge of 4% DSS.

Results: 2% DSS-mice injected with omentum cells exhibited a decrease in body weight loss, decreased inflammation in the colon and decreased levels of the inflammatory cytokine KC in the serum compared to mice given 2% DSS alone. In addition, mice administered a lethal dose of 4% DSS exhibited a 50% decrease in mortality when injected with omentum cells.

Conclusion: Cells from the omentum exert anti-inflammatory and/or healing properties in the acute DSS-induced colitis model.

No MeSH data available.


Related in: MedlinePlus