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Omentum Cells Promote Healing of Colonic Tissues in Dextran Sulfate Sodium (DSS) Induced Model of Colitis in Mice.

Jung BC, Lee MH, Sethupathi P, Lee IS, Lee D, Rhee KJ - J Lifestyle Med (2013)

Bottom Line: Inflammatory bowel disease is characterized by persistent inflammation of the intestinal tissues.Although the usage of biologics has greatly enhanced the management of this disorder, a permanent treatment does not exist.Thereafter, body weight change, serum KC levels, and histological analysis of the colon were conducted.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Korea.

ABSTRACT

Background: Inflammatory bowel disease is characterized by persistent inflammation of the intestinal tissues. Although the usage of biologics has greatly enhanced the management of this disorder, a permanent treatment does not exist. In this study, we investigated whether the cells with anti-inflammatory and healing properties from the omentum could be harnessed to treat colitis in dextran sulfate sodium (DSS)-induced mouse colitis model.

Methods: C57BL/6 mice were administered 2% DSS for 10 days and then injected in the peritoneum with cells isolated from the murine omentum. Thereafter, body weight change, serum KC levels, and histological analysis of the colon were conducted. We also examined if omentum infused mice were resistant to a lethal challenge of 4% DSS.

Results: 2% DSS-mice injected with omentum cells exhibited a decrease in body weight loss, decreased inflammation in the colon and decreased levels of the inflammatory cytokine KC in the serum compared to mice given 2% DSS alone. In addition, mice administered a lethal dose of 4% DSS exhibited a 50% decrease in mortality when injected with omentum cells.

Conclusion: Cells from the omentum exert anti-inflammatory and/or healing properties in the acute DSS-induced colitis model.

No MeSH data available.


Related in: MedlinePlus

Omentum cells alleviate body weight loss. Mice were administerd 2% DSS for 10 days and then either injected intraperitoneally with omentum cells (5 × 106 cells/mouse)(2% DSS + omentum) or with mock injection of PBS alone (2% DSS). The body weight change was monitored for up to 28 days post DSS treatment. The daily body weight of individual mice was normalized to the starting body weight. Shown are pooled data from two independent experiments with a total of 10 mice per group. *p-value <0.05 using the Mann-Whitney U test.
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f1-jlm-03-48: Omentum cells alleviate body weight loss. Mice were administerd 2% DSS for 10 days and then either injected intraperitoneally with omentum cells (5 × 106 cells/mouse)(2% DSS + omentum) or with mock injection of PBS alone (2% DSS). The body weight change was monitored for up to 28 days post DSS treatment. The daily body weight of individual mice was normalized to the starting body weight. Shown are pooled data from two independent experiments with a total of 10 mice per group. *p-value <0.05 using the Mann-Whitney U test.

Mentions: DSS induces diarrhea, gross rectal bleeding, and weight loss in mice [19]. Histologically, DSS induces mucosal ulcerations, dysplasia and formation of crypt abscesses. We tested the effect of omentum cells in colonic tissue repair by using the DSS-induced colitis model. To prepare sufficient numbers of omentum cells, the omentum was expanded in mice by intraperitoneal injection of polyacrylamide beads as reported for rats [14]. Seven days after injection of polyacrylamide beads, omentum mass was grossly increased in the peritoneum (data not shown). To examine if omentum cells could alleviate DSS-induced colitis, mice were given 2% DSS in the drinking water for 10 days and then injected intraperitoneally with mononuclear cells isolated from the omentum (5 × 106 cells/mouse). Control mice (mock group) were given 2% DSS for 10 days and then injected with PBS alone. Both groups of mice exhibited a 3% decrease in initial body weight at day 10 post-DSS treatment and 5% decrease in initial body weight at day 14 (Fig. 1). Thereafter, the body weights of the two groups diverged: the omentum-injected mice had a gradual increase in body weight whereas the mock-injected mice continued to lose weight up to day 21.


Omentum Cells Promote Healing of Colonic Tissues in Dextran Sulfate Sodium (DSS) Induced Model of Colitis in Mice.

Jung BC, Lee MH, Sethupathi P, Lee IS, Lee D, Rhee KJ - J Lifestyle Med (2013)

Omentum cells alleviate body weight loss. Mice were administerd 2% DSS for 10 days and then either injected intraperitoneally with omentum cells (5 × 106 cells/mouse)(2% DSS + omentum) or with mock injection of PBS alone (2% DSS). The body weight change was monitored for up to 28 days post DSS treatment. The daily body weight of individual mice was normalized to the starting body weight. Shown are pooled data from two independent experiments with a total of 10 mice per group. *p-value <0.05 using the Mann-Whitney U test.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390752&req=5

f1-jlm-03-48: Omentum cells alleviate body weight loss. Mice were administerd 2% DSS for 10 days and then either injected intraperitoneally with omentum cells (5 × 106 cells/mouse)(2% DSS + omentum) or with mock injection of PBS alone (2% DSS). The body weight change was monitored for up to 28 days post DSS treatment. The daily body weight of individual mice was normalized to the starting body weight. Shown are pooled data from two independent experiments with a total of 10 mice per group. *p-value <0.05 using the Mann-Whitney U test.
Mentions: DSS induces diarrhea, gross rectal bleeding, and weight loss in mice [19]. Histologically, DSS induces mucosal ulcerations, dysplasia and formation of crypt abscesses. We tested the effect of omentum cells in colonic tissue repair by using the DSS-induced colitis model. To prepare sufficient numbers of omentum cells, the omentum was expanded in mice by intraperitoneal injection of polyacrylamide beads as reported for rats [14]. Seven days after injection of polyacrylamide beads, omentum mass was grossly increased in the peritoneum (data not shown). To examine if omentum cells could alleviate DSS-induced colitis, mice were given 2% DSS in the drinking water for 10 days and then injected intraperitoneally with mononuclear cells isolated from the omentum (5 × 106 cells/mouse). Control mice (mock group) were given 2% DSS for 10 days and then injected with PBS alone. Both groups of mice exhibited a 3% decrease in initial body weight at day 10 post-DSS treatment and 5% decrease in initial body weight at day 14 (Fig. 1). Thereafter, the body weights of the two groups diverged: the omentum-injected mice had a gradual increase in body weight whereas the mock-injected mice continued to lose weight up to day 21.

Bottom Line: Inflammatory bowel disease is characterized by persistent inflammation of the intestinal tissues.Although the usage of biologics has greatly enhanced the management of this disorder, a permanent treatment does not exist.Thereafter, body weight change, serum KC levels, and histological analysis of the colon were conducted.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Korea.

ABSTRACT

Background: Inflammatory bowel disease is characterized by persistent inflammation of the intestinal tissues. Although the usage of biologics has greatly enhanced the management of this disorder, a permanent treatment does not exist. In this study, we investigated whether the cells with anti-inflammatory and healing properties from the omentum could be harnessed to treat colitis in dextran sulfate sodium (DSS)-induced mouse colitis model.

Methods: C57BL/6 mice were administered 2% DSS for 10 days and then injected in the peritoneum with cells isolated from the murine omentum. Thereafter, body weight change, serum KC levels, and histological analysis of the colon were conducted. We also examined if omentum infused mice were resistant to a lethal challenge of 4% DSS.

Results: 2% DSS-mice injected with omentum cells exhibited a decrease in body weight loss, decreased inflammation in the colon and decreased levels of the inflammatory cytokine KC in the serum compared to mice given 2% DSS alone. In addition, mice administered a lethal dose of 4% DSS exhibited a 50% decrease in mortality when injected with omentum cells.

Conclusion: Cells from the omentum exert anti-inflammatory and/or healing properties in the acute DSS-induced colitis model.

No MeSH data available.


Related in: MedlinePlus