Identification of vitamin C transporters in the human airways: a cross-sectional in vivo study.
Bottom Line: As secondary outcome measures, RTLF vitamin C concentration was measured and related to transporter expression, as well as bronchial epithelial inflammatory and goblet cells numbers.This experimental study is the first to demonstrate protein expression of GLUT2 and SVCT2 in the human bronchial epithelium.A negative correlation between SVCT2-positive goblet cells and bronchial RTLF vitamin C concentrations suggests a possible role for goblet cells in regulating the extracellular vitamin C pool.
Affiliation: Department of Public Health and Clinical Medicine, Division of Medicine/Respiratory Medicine and Allergy, Umeå University, Umeå, Sweden.Show MeSH
Related in: MedlinePlus
Mentions: Positive staining was found in blood vessels for SVCT1 and 2, with localised staining for SVCT2 also observed in the apical epithelium (figure 2). No difference in the epithelial expression of SVCT1 or 2 was observed between the patients with asthma and healthy subjects (table 3). Similarly, no difference in SVCT staining was observed in leucocyte subsets by FACS (see online supplementary table S2). Within the bronchial epithelium, the localisation of SVCT2 staining to goblet-like cells was confirmed using PAS, to identify mucin-containing cells, and SVCT2 in consecutive and double-stained sections. These analyses were performed on 10 biopsies, five from the healthy group and five from the asthmatics (figure 2). The selected samples were chosen to encompass the full range of measured vitamin C concentrations within the bronchial RTLFs. No significant differences in the number of goblet cells or SVCT2-positive goblet cells were observed between the two groups (table 4).
Affiliation: Department of Public Health and Clinical Medicine, Division of Medicine/Respiratory Medicine and Allergy, Umeå University, Umeå, Sweden.