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The rice ALS3 encoding a novel pentatricopeptide repeat protein is required for chloroplast development and seedling growth.

Lin D, Gong X, Jiang Q, Zheng K, Zhou H, Xu J, Teng S, Dong Y - Rice (N Y) (2015)

Bottom Line: Moreover, expression analysis revealed that the asl3 mutation severely affected the transcriptional levels of important genes associated with plastid translation machinery and photosynthesis, which may impair photosynthesis and finally led to the seedling death in asl3 mutant.These results evidenced the important role of ASL3 in the early development of rice, especially chloroplast development.Disruption of the ASL3 would lead to a defective chloroplast and seedling lethality, and affected expression levels of genes associated with chloroplast development and photosynthesis at early leaf stage of rice.

View Article: PubMed Central - PubMed

Affiliation: Development Center of Plant Germplasm Resources, College of Life and Environment Sciences, Shanghai Normal University, Shanghai, 200234 China.

ABSTRACT

Background: Pentatricopeptide repeat (PPR) proteins play essential roles in modulating the expression of organelle genes and have expanded greatly in higher plants. However, molecular mechanisms of most rice PPR genes remain unclear.

Results: In this study, a new rice PPR mutant, asl3 (albino seedling lethality3) exhibits an albino lethal phenotype at the seedling stage. This albino phenotype was associated with altered photosynthetic-pigment and chloroplast development. Map-based cloning showed that ASL3 encodes a novel rice PPR protein with 10 tandem PPR motifs, which localizes to the chloroplast. ASL3 showed tissue-specific expression, as it was highly expressed in the chlorenchyma, but expressed at much lower levels in roots and panicles. RNAi of ASL3 confirmed that ASL3 plays an essential role in the early development and chloroplast development in rice. Moreover, expression analysis revealed that the asl3 mutation severely affected the transcriptional levels of important genes associated with plastid translation machinery and photosynthesis, which may impair photosynthesis and finally led to the seedling death in asl3 mutant. These results evidenced the important role of ASL3 in the early development of rice, especially chloroplast development.

Conclusions: The ASL3 gene encoded a novel chloroplast-targeted PPR protein with 10 tandem PPR motifs in rice. Disruption of the ASL3 would lead to a defective chloroplast and seedling lethality, and affected expression levels of genes associated with chloroplast development and photosynthesis at early leaf stage of rice.

No MeSH data available.


Related in: MedlinePlus

Phylogenic analysis of ASL3 Protein: (A) Amino acid sequence alignment of its homologsArabidopsis thaliana,Brachypodium distachyon,Sorghum bicolorandZea mays. Amino acids fully or semi-conserved are shaded black and gray, respectively; (B) Homologous proteins similar to ASL3 were used to obtain a phylogenetic tree with the program Mega5.1, which was bootstrapped over 1,000 cycles. Significance values above a 50% cutoff threshold are indicated near the relative branches.
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Fig5: Phylogenic analysis of ASL3 Protein: (A) Amino acid sequence alignment of its homologsArabidopsis thaliana,Brachypodium distachyon,Sorghum bicolorandZea mays. Amino acids fully or semi-conserved are shaded black and gray, respectively; (B) Homologous proteins similar to ASL3 were used to obtain a phylogenetic tree with the program Mega5.1, which was bootstrapped over 1,000 cycles. Significance values above a 50% cutoff threshold are indicated near the relative branches.

Mentions: Orthologs of ASL3 from Arabidopsis thaliana, Brachypodium distachyon, Sorghum bicolor and Zea mays were found in the NCBI database. ASL3 has 42–74% amino acid sequence identity to the four characterized orthologs. Among these, ASL3 exhibits maximum sequence similarity with protein in Brachypodium distachyon, with 74% amino acid identity and it shared 42% peptide identity with protein from Arabidopsis (Figure 5A). These data indicated that the ASL3 protein is highly conserved in higher plants. Eight related proteins were used to investigate the relationship between ASL3 homologs in evolutionary history. As shown in Figure 5B, they could be divided into two groups: (1) the orthologs proteins from both monocots and dicots are divided clearly into two subgroups; (2) another two paralogous proteins from rice and Arabidopsis forms another group.Figure 5


The rice ALS3 encoding a novel pentatricopeptide repeat protein is required for chloroplast development and seedling growth.

Lin D, Gong X, Jiang Q, Zheng K, Zhou H, Xu J, Teng S, Dong Y - Rice (N Y) (2015)

Phylogenic analysis of ASL3 Protein: (A) Amino acid sequence alignment of its homologsArabidopsis thaliana,Brachypodium distachyon,Sorghum bicolorandZea mays. Amino acids fully or semi-conserved are shaded black and gray, respectively; (B) Homologous proteins similar to ASL3 were used to obtain a phylogenetic tree with the program Mega5.1, which was bootstrapped over 1,000 cycles. Significance values above a 50% cutoff threshold are indicated near the relative branches.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4390607&req=5

Fig5: Phylogenic analysis of ASL3 Protein: (A) Amino acid sequence alignment of its homologsArabidopsis thaliana,Brachypodium distachyon,Sorghum bicolorandZea mays. Amino acids fully or semi-conserved are shaded black and gray, respectively; (B) Homologous proteins similar to ASL3 were used to obtain a phylogenetic tree with the program Mega5.1, which was bootstrapped over 1,000 cycles. Significance values above a 50% cutoff threshold are indicated near the relative branches.
Mentions: Orthologs of ASL3 from Arabidopsis thaliana, Brachypodium distachyon, Sorghum bicolor and Zea mays were found in the NCBI database. ASL3 has 42–74% amino acid sequence identity to the four characterized orthologs. Among these, ASL3 exhibits maximum sequence similarity with protein in Brachypodium distachyon, with 74% amino acid identity and it shared 42% peptide identity with protein from Arabidopsis (Figure 5A). These data indicated that the ASL3 protein is highly conserved in higher plants. Eight related proteins were used to investigate the relationship between ASL3 homologs in evolutionary history. As shown in Figure 5B, they could be divided into two groups: (1) the orthologs proteins from both monocots and dicots are divided clearly into two subgroups; (2) another two paralogous proteins from rice and Arabidopsis forms another group.Figure 5

Bottom Line: Moreover, expression analysis revealed that the asl3 mutation severely affected the transcriptional levels of important genes associated with plastid translation machinery and photosynthesis, which may impair photosynthesis and finally led to the seedling death in asl3 mutant.These results evidenced the important role of ASL3 in the early development of rice, especially chloroplast development.Disruption of the ASL3 would lead to a defective chloroplast and seedling lethality, and affected expression levels of genes associated with chloroplast development and photosynthesis at early leaf stage of rice.

View Article: PubMed Central - PubMed

Affiliation: Development Center of Plant Germplasm Resources, College of Life and Environment Sciences, Shanghai Normal University, Shanghai, 200234 China.

ABSTRACT

Background: Pentatricopeptide repeat (PPR) proteins play essential roles in modulating the expression of organelle genes and have expanded greatly in higher plants. However, molecular mechanisms of most rice PPR genes remain unclear.

Results: In this study, a new rice PPR mutant, asl3 (albino seedling lethality3) exhibits an albino lethal phenotype at the seedling stage. This albino phenotype was associated with altered photosynthetic-pigment and chloroplast development. Map-based cloning showed that ASL3 encodes a novel rice PPR protein with 10 tandem PPR motifs, which localizes to the chloroplast. ASL3 showed tissue-specific expression, as it was highly expressed in the chlorenchyma, but expressed at much lower levels in roots and panicles. RNAi of ASL3 confirmed that ASL3 plays an essential role in the early development and chloroplast development in rice. Moreover, expression analysis revealed that the asl3 mutation severely affected the transcriptional levels of important genes associated with plastid translation machinery and photosynthesis, which may impair photosynthesis and finally led to the seedling death in asl3 mutant. These results evidenced the important role of ASL3 in the early development of rice, especially chloroplast development.

Conclusions: The ASL3 gene encoded a novel chloroplast-targeted PPR protein with 10 tandem PPR motifs in rice. Disruption of the ASL3 would lead to a defective chloroplast and seedling lethality, and affected expression levels of genes associated with chloroplast development and photosynthesis at early leaf stage of rice.

No MeSH data available.


Related in: MedlinePlus